nitrogen-dioxide has been researched along with phenylpyruvic-acid* in 1 studies
1 other study(ies) available for nitrogen-dioxide and phenylpyruvic-acid
| Article | Year |
|---|---|
Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions.
The use of engineered phenylalanine dehydrogenase N145A supported on Celite for the reductive amination of phenylpyruvic acid in homogeneous and biphasic aqueous-organic solvents is reported. The results indicate that the immobilised biocatalyst is remarkably robust, even in the presence of high concentrations of polar or non-polar organic solvents such as acetone, methanol, n-hexane, toluene and methylene chloride. Cofactor regeneration with alcohol dehydrogenase from Saccharomyces cerevisiae and ethanol was successfully explored. Application to the non-natural poorly water-soluble 2-oxo acid p-NO(2)-phenylpyruvic acid was successfully performed, resulting in the biocatalytic synthesis of p-NO(2)-phenylalanine. In all cases 100% stereoselectivity for the production of the amino acid was retained. Topics: Amino Acid Oxidoreductases; Chemistry, Organic; Molecular Structure; Nitrogen Dioxide; Organic Chemistry Phenomena; Phenylalanine; Phenylpyruvic Acids; Protein Engineering; Solvents | 2005 |