nitroarginine and 14-15-epoxy-5-8-11-eicosatrienoic-acid

Acetylcholine-induced endothelium-dependent relaxation in the renal afferent arteriole has been ascribed to nitric oxide, but the role of endothelium-derived hyperpolarizing factors (EDHFs) and 14,15-epoxyeicosatrienoic acid (14,15-EET) are unclear.. Single afferent arterioles were dissected from kidney of normal rabbits and microperfused in vitro at 60 mm Hg. Vessels were preconstricted submaximally with norepinephrine (10(-8) mol/L). Relaxation was assessed following cumulative addition of ACh (10(-9) to 10(-4) mol/L) alone, or in the presence of indomethacin (to inhibit cyclooxygenase), Nw-nitro-L-arginine (L-NNA) (to inhibit nitric oxide synthase), methylene blue (to inhibit soluble guanylate cyclase), or a combination of L-NNA + methylene blue. To assess contributions by EDHF, studies were repeated with either apamin + charybdotoxin [to block Ca2+-activated K+ channels (KCa)] or with 40 mmol/L KCl. To asses the role of 14,15-EET, relaxations were evaluated in the presence of its competitive inhibitor 14,15-epoxyeicosa-5(Z)-enoic acid (14,15-EEZE).. Relaxation by acetylcholine was abolished following endothelial denudation. It was unaffected by indomethacin but was inhibited 54%+/- 5% (P < 0.001) by L-NNA, 57%+/- 5% by methylene blue, and 60%+/- 4% by the combination of L-NNA plus methylene blue. Relaxation was inhibited further by KCl (80%+/- 6%) or by apamin + charybdotoxin (96%+/- 2%). 14,15-EEZE, alone, inhibited acetylcholine-induced relaxation by 29%+/- 3%, and by 80%+/- 5% in the presence of L-NNA.. Acetylcholine-induced afferent arteriolar relaxation depends strongly on both nitric oxide, acting via soluble guanylate cyclase, and on an EDHF, likely 14,15-EET, acting via KCa.

Topics: 8,11,14-Eicosatrienoic Acid; Acetylcholine; Animals; Apamin; Arterioles; Biological Factors; Charybdotoxin; Endothelium, Vascular; Enzyme Inhibitors; Male; Nitric Oxide; Nitroarginine; Nitroprusside; Potassium Channels, Calcium-Activated; Rabbits; Renal Circulation; Vasodilation; Vasodilator Agents