nitrilotriacetic acid has been researched along with nickel in 124 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 6 (4.84) | 18.7374 |
| 1990's | 23 (18.55) | 18.2507 |
| 2000's | 54 (43.55) | 29.6817 |
| 2010's | 36 (29.03) | 24.3611 |
| 2020's | 5 (4.03) | 2.80 |
| Authors | Studies |
|---|---|
| Nakon, R; Newlin, DE; Pellack, MA | 1 |
| Hochuli, E | 1 |
| Döbeli, H; Hochuli, E; Schacher, A | 1 |
| Dalvit, C; Hegetschweiler, K; Saltman, P; Wright, PE | 1 |
| Dalela, RC; Jain, M; Verma, SR | 1 |
| Partridge, CD; Yates, MG | 1 |
| Botting, CH; Randall, RE | 1 |
| Jørgensen, M; Koch, T; Larsson, C; Nielsen, PE; Orum, H; Stanley, C | 1 |
| Arimilli, S; Deshpande, SV; Kendrick, T; Mukku, PV; Nag, B; Sharma, SD | 1 |
| Inoue, S; Kawanishi, S; Yamamoto, K | 1 |
| Crowe, J; Döbeli, H; Gentz, R; Henco, K; Hochuli, E; Stüber, D | 1 |
| Imperial, J; Palacios, JM; Rey, L; Ruiz-Argüeso, T | 1 |
| Island, MD; Maneval, D; Mobley, HL; Sriwanthana, B | 1 |
| Gu, J; Iadarola, MJ; Stephenson, CG | 1 |
| Hess, H; Schmitt, J; Stunnenberg, HG | 1 |
| Finbow, ME; Findlay, JB; Harrison, MA; Jones, PC; Kim, YI | 1 |
| Burgess, RR; McMahan, SA | 1 |
| Crowe, J; Masone, BS; Ribbe, J | 1 |
| Dougherty, JP; Dunn, KE; Gibbs, CS; Paborsky, LR | 1 |
| Ji, Z; Miller, LJ; Pinon, DI | 1 |
| Barisani, D; Wessling-Resnick, M | 1 |
| Hidaka, Y; Inouye, M; Park, H | 1 |
| Edebratt, F; Hämäläinen, M; Hansson, A; Karlsson, AF; Lidholm, J; Magnusson, K; Nieba, L; Nieba-Axmann, SE; Persson, A; Plückthun, A | 1 |
| Atkins, WM; Chen, JP; Chin, WC; Dabrowski, MJ; Shi, H | 1 |
| Gal-On, A; Huet, H; Kadouri, D; Peng, Y; Raccah, B; Singer, S; Wang, Y | 1 |
| Buus, S; Hansen, NJ; Nielsen, LL; Pedersen, LO; Sylvester-Hvid, C | 1 |
| Freimuth, P; Hainfeld, JF; Halsey, CM; Liu, W; Powell, RD | 1 |
| Beresten, SF; Ellis, NA; Naureckiene, S; Stan, R; van Brabant, AJ; Ye, T | 1 |
| Cocca, BA; Radic, MZ; Seal, SN | 1 |
| Hasegawa, M; Miyake, J; Nakamura, C; Yasuda, Y | 1 |
| Baumann, CG; Ferguson, AL; Hoggett, JG; Hughes, AD; Scott, DJ; Tufail, U | 1 |
| Colpas, GJ; Hausinger, RP; Soriano, A | 1 |
| Bornhorst, JA; Falke, JJ | 1 |
| Andreana, P; Chen, X; Fang, J; Kowal, P; Liu, Z; Shao, J; Wang, PG; Zhang, J | 1 |
| Ebright, RH; Ebright, YW; Kapanidis, AN | 1 |
| Minneman, KP; Robeva, A; Rogge, G; Uberti, M; Vicentic, A | 1 |
| Anthony, LC; Bergendahl, V; Burgess, RR; Heyduk, T | 1 |
| Unrau, PJ; Wang, QS | 1 |
| Ngamkitidechakul, C; Twining, SS | 1 |
| Alloza, I; Hawthorne, S; Martens, E; Vandenbroeck, K | 1 |
| Chen, J; Chen, K; Jiang, H; Luo, H; Peng, S; Qin, J; Shen, J; Shen, X; Sun, H; Sun, T; Wang, Y; Xie, Y; Yang, Y; Yu, C | 1 |
| Javitch, JA | 1 |
| Campbell, RL; Davies, PL; Diaz, BG; Kuiper, MJ; Moldoveanu, T | 1 |
| Lebedev, N; Shashidhar, R; Trammell, SA; Wang, L; Zullo, JM | 1 |
| Allen, TM; Das, D; Suresh, MR | 1 |
| Johnson, DL; Martin, LL | 1 |
| Bernaudat, F; Büllow, L | 1 |
| Blankespoor, R; Limoges, B; Schöllhorn, B; Syssa-Magalé, JL; Yazidi, D | 1 |
| Kang, SM; Kim, JH; Oh, JW; Shin, MJ | 1 |
| Gandolfi, AJ; Kirkpatrick, DS; Liebler, DC; Tsaprailis, G; Weldon, SF | 1 |
| Brock, R; Lata, S; Piehler, J; Reichel, A; Tampé, R | 1 |
| Bumba, L; Dobakova, M; Komenda, J; Tichy, M; Vacha, F | 1 |
| Goldsmith, CR; Jaworski, J; Lippard, SJ; Sheng, M | 1 |
| Klenkar, G; Liedberg, B; Piehler, J; Tampé, R; Tinazli, A; Valiokas, R | 1 |
| Boos, M; Fiedler, M; Mihailova, M; Petrovskis, I; Pumpens, P; Roggendorf, M; Sominskaya, I; Viazov, S | 1 |
| Bumba, L; Komenda, J; Nebesarova, J; Promnares, K; Tichy, M; Vacha, F | 1 |
| Appel, J; Gutekunst, K; Hoffmann, D; Klissenbauer, M; Schulz-Friedrich, R | 1 |
| Franses, EI; Holland, DP; Kang, E; Kim, JM; Lee, GU; McClellan, SJ; Park, JW; Park, K; Thompson, DH | 1 |
| Escalante, M; Huskens, J; Maury, P; Péter, M; Reinhoudt, DN; Subramaniam, V | 1 |
| Walkinshaw, MD; Wear, MA | 1 |
| Baker, GL; Bruening, ML; Dai, J; Jain, P; Sun, L | 1 |
| Dräger, G; Kirschning, A; Kiss, C; Kunz, U | 1 |
| Huskens, J; Ludden, MJ; Mulder, A; Schulze, K; Subramaniam, V; Tampé, R | 1 |
| Ayres, L; Bergia, A; Nolte, RJ; Rowan, AE; Samorì, B; Valle, F; Zuccheri, G | 1 |
| Petty, KJ | 2 |
| Dukovski, D; Kelly, DF; Walz, T | 2 |
| Börsch, M; Duncan, TM; Herrmann, A; Mihov, G; Müllen, K; Peneva, K; Zarrabi, N | 1 |
| Grailhe, R; Kim, J; Kwon, DY; Park, HY; Ryu, J; Song, R | 1 |
| Han, SJ; Hong, S; Kim, J; Kim, MJ; Park, HY; Ryu, J; Song, R | 1 |
| Jung, H; Jung, SY; Kang, SH; Kim, JD; Kim, KH; Kim, YJ | 1 |
| Campopiano, DJ; Caniard, A; Gupta, M; Mareque-Rivas, JC; Touceda-Varela, A | 1 |
| Chen, JH; Cheng, FY; Hwu, JR; Shieh, DB; Su, CH; Su, WC; Tsai, TL; Tzeng, Y; Weng, JC; Wu, PC; Yeh, CS | 1 |
| Arcari, P; Chambery, A; Di Maro, A; Lamberti, A; Longo, O; Masullo, M; Parente, A; Sanges, C | 1 |
| De, M; Rana, S; Rotello, VM | 1 |
| Casse, O; Meier, W; Nehring, R; Palivan, CG; Tanner, P; Tuxen, J | 1 |
| Clarke, S; Dahan, M; Gouzer, GG; Marchi-Artzner, V; Piehler, J; Pinaud, F; Roullier, V; Schaible, D; You, C | 1 |
| Erben, CM; Goodman, RP; Ho, WM; Kapanidis, AN; Malo, J; McKee, ML; Turberfield, AJ | 1 |
| Augusto, EF; Borsoi-Ribeiro, M; Bresolin, IT; Bueno, SM; Tamashiro, WM; Vijayalakshmi, MA | 1 |
| Hess, H | 1 |
| Bergkvist, M; Mukai, C; Nelson, JL; Travis, AJ | 1 |
| Fujishima, SH; Hamachi, I; Nonaka, H; Ojida, A; Tsukiji, S; Uchinomiya, SH | 1 |
| Baati, R; Barlaam, B; Brellier, M; Mioskowski, C | 1 |
| Bujoli, B; Cinier, M; Fabre, RM; Pecorari, F; Petit, M; Talham, DR; Tellier, C; Williams, MN | 1 |
| Baker, GL; Bruening, ML; Geiger, JH; Jain, P; Vyas, MK | 1 |
| Geil, B; Janshoff, A; Keller, R; Lorenz, B; Sunnick, E | 1 |
| Iversen, L; Liu, YC; Martinez, KL; Nygård, J; Park, J; Rieben, N; Sørensen, BS | 1 |
| Andresen, TL; Daugaard, AE; Hansen, TS; Hvilsted, S; Larsen, NB; Lind, JU | 1 |
| Chen, LQ; Hu, PP; Huang, CZ; Li, YF; Liu, C; Peng, L; Xiao, SJ; Zhen, SJ | 1 |
| Choi, Y; Hong, S; Kim, H; Kim, J; Kwon, H; Song, R | 1 |
| Li, Z; Wang, DI; Wang, W | 1 |
| Baumgaertner, P; Guillaume, P; Irving, M; Luescher, IF; Schmidt, J; Speiser, D | 1 |
| Gulka, CP; Haselton, FR; Swartz, JD; Wright, DW | 1 |
| Benhabbour, SR; DeSimone, JM; Jain, A; Kim, D; Liu, R; Luft, JC; Mumper, RJ; Parrott, MC; Wadhwa, S | 1 |
| Hanaoka, K; Hirabayashi, K; Komatsu, T; Nagano, T; Shimonishi, M; Terai, T; Ueno, T | 1 |
| Chen, G; Cui, F; Lan, W; Liu, R; Tan, F; Yushupujiang, M | 1 |
| Garmo, ØA; Lehto, N; Shafaei Arvajeh, MR; Zhang, H | 1 |
| Fang, TY; Su, LY; Tseng, WC | 1 |
| Ali, M; Andre, R; Ensinger, W; Müller, WE; Schröder, HC; Tahir, MN; Tremel, W | 1 |
| Appelhans, D; Hauptmann, N; Komber, H; Muñoz-Fernández, MÁ; Pion, M; Voit, B; Werner, C | 1 |
| Day, JW; Kim, CH; Schultz, PG; Smider, VV | 1 |
| Hayat, A; Marty, JL; Sassolas, A | 1 |
| Berthing, T; Frederiksen, RS; Holm, J; Liu, YC; Madsen, MH; Martinez, KL; Nygård, J; Rostgaard, KR | 1 |
| Lai, RY; Zaitouna, AJ | 1 |
| Hellmert, M; Korth, C; Müller-Schiffmann, A; Peters, MS; Schrader, T | 1 |
| Dogterom, M; Lamers, D; Lof, A; Roth, S; Taberner, N; Zeijlemaker, H | 1 |
| Argüello, JM; Padilla-Benavides, T | 1 |
| Bhat, A; Bieling, P; Husain, K; Iljazi, E; Köster, DV; Mayor, S; Mullins, RD; Rao, M | 1 |
| Kamiya, N; Kimizuka, N; Matsuura, K; Minamihata, K; Nakamura, T; Noguchi, T; Watanabe, K | 1 |
| Chmurzyński, L; Jacewicz, D; Muñoz, E; Pilarski, B; Pranczk, J; Tesmar, A; Wyrzykowski, D | 1 |
| Ali, M; Duznovic, I; Ensinger, W; Mafe, S; Nasir, S; Ramirez, P | 1 |
| Blum, C; Cabanas-Danés, J; Cazade, PA; Huskens, J; Jonkheijm, P; O'Mahony, S; Prangsma, J; Subramaniam, V; Thompson, D; Tromp, E; Wasserberg, D | 1 |
| Cabanas-Danés, J; Huskens, J; Jonkheijm, P; Subramaniam, V; Wasserberg, D | 1 |
| Chen, J; Chen, X; Hu, X; Liu, X; Yuan, X; Zhang, X | 1 |
| An, SSA; Hwang, A; Jose, L; Lee, C; Paik, HJ; Park, JH; Shim, K; Song, JK | 1 |
| Liu, L; Liu, Q; Tan, X; Wang, X; Zhou, F | 1 |
| Chang, YY; Jiang, N; Lai, YT; Li, H; Sun, H; Yang, X; Yang, Y | 1 |
| Hashimoto, T; Matsui, T; Ogawa, T; Tanaka, Y; Ui, M; Ye, Y | 1 |
| Asano, K; Kubota, R; Minami, T; Minamiki, T; Sasaki, Y | 1 |
| Ani, M; Boonrod, K; Braun, M; Krczal, G; Müller-Renno, C; Rink, V; Ziegler, C | 1 |
| Hamachi, I; Heroven, C; Hoon Oh, J; Radu Aricescu, A; Tamura, T; Thimaradka, V; Yuzaki, M | 1 |
| Gao, X; Han, L; Ji, F; Jia, L; Meng, X; Ren, J; Xiang, X | 1 |
| Hao, Y; Liu, P; Lv, Y; Ma, X; Xia, N | 1 |
2 review(s) available for nitrilotriacetic acid and nickel
| Article | Year |
|---|---|
6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification.
Topics: Chelating Agents; Chromatography, Affinity; Escherichia coli; Genetic Vectors; Histidine; Nickel; Nitrilotriacetic Acid; Operator Regions, Genetic; Promoter Regions, Genetic; Recombinant Fusion Proteins | 1994 |
Protein Assays on Organic Electronics: Rational Device and Material Designs for Organic Transistor-Based Sensors.
Topics: Aptamers, Peptide; Biosensing Techniques; Electrochemical Techniques; Electrodes; Humans; Immobilized Proteins; Nickel; Nitrilotriacetic Acid; Organic Chemicals; Receptors, Artificial; Surface Properties; Transistors, Electronic | 2020 |
122 other study(ies) available for nitrilotriacetic acid and nickel
| Article | Year |
|---|---|
Kinetics and mechanism of tetradentate nickel(II) chelate and metal nitrilotriacetate promoted hydrolysis of methyl glycinate: an isokinetic relationship.
Topics: Acetates; Catalysis; Chelating Agents; Glycine; Hydrolysis; Kinetics; Nickel; Nitrilotriacetic Acid; Sarcosine | 1977 |
Large-scale chromatography of recombinant proteins.
Topics: Chromatography, Affinity; Copper; Endotoxins; Escherichia coli; Humans; Immunoglobulins; Immunosorbent Techniques; Interferon Type I; Nickel; Nitrilotriacetic Acid; Polysorbates; Recombinant Proteins | 1988 |
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
Topics: Adsorption; Animals; Chelating Agents; Histidine; Ligands; Metals; Nickel; Nitrilotriacetic Acid; Peptides; Proteins; Spectrophotometry, Atomic | 1987 |
Kinetics and mechanisms of the oxidation of myoglobin by Fe(III) and Cu(II) complexes.
Topics: Binding Sites; Copper; Electron Transport; Ferric Compounds; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Myoglobin; Nickel; Nitrilotriacetic Acid; Osmolar Concentration; Oxidation-Reduction; Zinc | 1987 |
In vivo removal of a few heavy metals in certain tissues of the fish Notopterus notopterus.
Topics: Animals; Chelating Agents; Chromium; Ditiocarb; Edetic Acid; Fishes; Mercury; Metals; Nickel; Nitrilotriacetic Acid; Pentetic Acid; Water Pollutants; Water Pollutants, Chemical | 1981 |
Effect of chelating agents on hydrogenase in Azotobacter chroococcum. Evidence that nickel is required for hydrogenase synthesis.
Topics: Azotobacter; Chelating Agents; Hydrogenase; Iron; Nickel; Nitrilotriacetic Acid; Oxidoreductases; Trace Elements | 1982 |
Reporter enzyme-nitrilotriacetic acid-nickel conjugates: reagents for detecting histidine-tagged proteins.
Topics: Affinity Labels; Alkaline Phosphatase; Antibodies, Monoclonal; Chromatography, Affinity; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Indicators and Reagents; Nickel; Nitrilotriacetic Acid; Recombinant Proteins | 1995 |
Sequence-specific purification of nucleic acids by PNA-controlled hybrid selection.
Topics: Base Composition; Base Sequence; Binding Sites; Chromatography, Affinity; Histidine; Hot Temperature; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Nucleic Acid Hybridization; Nucleic Acids; Oligonucleotide Probes; Oligonucleotides; Peptides; Recombinant Fusion Proteins; RNA | 1995 |
Separation of complexes of major histocompatibility class II molecules and known antigenic peptide by metal chelate affinity chromatography.
Topics: Amino Acid Sequence; Antigen-Presenting Cells; Antigens; Cell Line; Cell Transformation, Viral; Chelating Agents; Chromatography, Affinity; Chromatography, High Pressure Liquid; Electrophoresis, Gel, Two-Dimensional; HLA-DR Antigens; Humans; Lymphocyte Activation; Molecular Sequence Data; Myelin Basic Protein; Nickel; Nitrilotriacetic Acid; Peptides; T-Lymphocytes | 1994 |
Active oxygen species in DNA damage induced by carcinogenic metal compounds.
Topics: Amino Acid Sequence; Carcinogens; Chromium; Cobalt; DNA Damage; Ferric Compounds; Free Radicals; Humans; Metals; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Reactive Oxygen Species | 1994 |
Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis.
Topics: Aerobiosis; Amino Acid Sequence; Amino Acids; Anaerobiosis; Bacterial Proteins; Carrier Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Escherichia coli Proteins; GTP-Binding Proteins; Hydrogenase; Isoelectric Point; Molecular Sequence Data; Molecular Weight; Nickel; Nitrilotriacetic Acid; Nucleotidases; Nucleotides; Pisum sativum; Recombinant Proteins; Rhizobium leguminosarum; Spectrophotometry, Ultraviolet; Symbiosis | 1994 |
Single-step purification of Proteus mirabilis urease accessory protein UreE, a protein with a naturally occurring histidine tail, by nickel chelate affinity chromatography.
Topics: Amino Acid Sequence; Bacterial Proteins; Carrier Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Genetic Complementation Test; Histidine; Molecular Sequence Data; Molecular Weight; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteus mirabilis; Recombinant Proteins; Sequence Analysis; Urease | 1994 |
Recombinant proteins attached to a nickel-NTA column: use in affinity purification of antibodies.
Topics: Antibodies; Chromatography, Affinity; Nickel; Nitrilotriacetic Acid; Recombinant Proteins | 1994 |
Affinity purification of histidine-tagged proteins.
Topics: Animals; Binding Sites; Chromatography, Affinity; Evaluation Studies as Topic; HeLa Cells; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Recombinant Proteins | 1993 |
Functional properties of a hybrid vacuolar H(+)-ATPase in Saccharomyces cells expressing the Nephrops 16-kDa proteolipid.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Base Sequence; Chromatography, Affinity; Crustacea; Gene Expression; Genes, Fungal; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutagenesis; Nickel; Nitrilotriacetic Acid; Protein Multimerization; Proteolipids; Proton-Translocating ATPases; Protons; Recombinant Proteins; Saccharomyces cerevisiae; Vacuoles; Vanadates | 1994 |
Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose.
Topics: Biotin; Blotting, Western; Collodion; HeLa Cells; Histidine; Humans; Lysine; Nickel; Nitrilotriacetic Acid; Recombinant Proteins; Saccharomyces cerevisiae; Sigma Factor | 1996 |
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Topics: Bacterial Proteins; Chromatography, Affinity; Escherichia coli; Genetic Testing; Genetic Vectors; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Recombinant Proteins; Resins, Plant; Solubility | 1996 |
A nickel chelate microtiter plate assay for six histidine-containing proteins.
Topics: Antibodies, Monoclonal; Chelating Agents; Enzyme-Linked Immunosorbent Assay; Histidine; Indicators and Reagents; Interleukin-8; Ligands; Lysine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Recombinant Proteins; Reproducibility of Results; Sensitivity and Specificity; Sequence Tagged Sites; Transferrin | 1996 |
Development of magnetic beads for rapid and efficient metal-chelate affinity purifications.
Topics: Chromatography, Affinity; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Polystyrenes; Resins, Synthetic; Tosyl Compounds | 1996 |
Fe-nitrilotriacetic acid--binding proteins associated with rat liver plasma membranes.
Topics: Animals; Carrier Proteins; Female; Ferric Compounds; Liver; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Rats; Rats, Sprague-Dawley | 1996 |
Demonstration of dimer formation of the cytoplasmic domain of a transmembrane osmosensor protein, EnvZ, of Escherichia coli using Ni-histidine tag affinity chromatography.
Topics: Bacterial Outer Membrane Proteins; Chromatography, Affinity; Cytoplasm; Escherichia coli; Escherichia coli Proteins; Gene Expression; Histidine; Multienzyme Complexes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Fusion Proteins; Resins, Plant | 1997 |
BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip.
Topics: Adenosine Triphosphate; Biosensing Techniques; Chaperonin 10; Chaperonin 60; Chelating Agents; Detergents; Edetic Acid; Histidine; Hydrogen-Ion Concentration; Kinetics; Ligands; Macromolecular Substances; Nickel; Nitrilotriacetic Acid; Polysorbates; Protein Binding; Protein Folding; Sodium Chloride; Surface Properties | 1997 |
Strategies for protein-based nanofabrication: Ni2+-NTA as a chemical mask to control biologically imposed symmetry.
Topics: Biocompatible Materials; Biosensing Techniques; Chromatography, Affinity; Dimerization; Escherichia coli; Glutamate-Ammonia Ligase; Microscopy, Electron; Models, Molecular; Molecular Probes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Protein Conformation | 1998 |
Affinity purification of HC-Pro of potyviruses with Ni2+-NTA resin.
Topics: Animals; Aphids; Chromatography, Affinity; Cucurbitaceae; Cysteine Endopeptidases; Immunoblotting; Nickel; Nitrilotriacetic Acid; Potyvirus; Resins, Plant; Viral Proteins; Virion | 1998 |
A single-chain fusion molecule consisting of peptide, major histocompatibility gene complex class I heavy chain and beta2-microglobulin can fold partially correctly, but binds peptide inefficiently.
Topics: Antigen Presentation; beta 2-Microglobulin; Hemagglutinin Glycoproteins, Influenza Virus; Histocompatibility Antigens Class I; Humans; Models, Immunological; Nickel; Nitrilotriacetic Acid; Peptide Fragments; Protein Binding; Protein Engineering; Protein Folding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins; Sepharose | 1999 |
Ni-NTA-gold clusters target His-tagged proteins.
Topics: Adenoviridae; Blotting, Western; Chromatography, Affinity; Forecasting; Gold; Histidine; Microscopy, Electron, Scanning Transmission; Molecular Probes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Receptors, Virus; Recombinant Proteins; Viral Structural Proteins | 1999 |
Purification of overexpressed hexahistidine-tagged BLM N431 as oligomeric complexes.
Topics: Adenosine Triphosphatases; Amino Acid Motifs; Animals; Bloom Syndrome; Chelating Agents; Chromatography, Affinity; Chromatography, Gel; DNA Helicases; Edetic Acid; Escherichia coli; Gene Expression; Histidine; Humans; Molecular Probes; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; RecQ Helicases; Solubility | 1999 |
Tandem affinity tags for the purification of bivalent anti-DNA single-chain Fv expressed in Escherichia coli.
Topics: Affinity Labels; Animals; Antibodies, Antinuclear; Antibody Specificity; Antigen-Antibody Reactions; Autoantibodies; Base Sequence; Chromatography, Affinity; Dimerization; DNA; DNA-Binding Proteins; Durapatite; Escherichia coli; Gene Expression; Humans; Immunoglobulin G; Immunoglobulin Variable Region; Leucine Zippers; Mice; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Periplasm; Protein Engineering; Recombinant Proteins; Sepharose | 1999 |
Self-assembling photosynthetic reaction centers on electrodes for current generation.
Topics: Biosensing Techniques; Electrochemistry; Electrodes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Photosynthetic Reaction Center Complex Proteins; Rhodobacter sphaeroides; Surface Plasmon Resonance | 2000 |
Interaction of sigma 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance.
Topics: Bacterial Proteins; Binding, Competitive; DNA-Directed RNA Polymerases; Enzyme Stability; Escherichia coli; Half-Life; Histidine; Holoenzymes; Kinetics; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Sigma Factor; Surface Plasmon Resonance | 2000 |
UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex.
Topics: Apoproteins; Bacterial Proteins; Bicarbonates; Carrier Proteins; Chelating Agents; Copper; Dose-Response Relationship, Drug; Enterobacter aerogenes; Enzyme Activation; Guanosine Triphosphate; Imino Acids; Nickel; Nitrilotriacetic Acid; Operon; Phosphate-Binding Proteins; Urease; Zinc | 2000 |
Purification of proteins using polyhistidine affinity tags.
Topics: Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Histidine; Indicators and Reagents; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Recombinant Proteins | 2000 |
Sugar nucleotide regeneration beads (superbeads): a versatile tool for the practical synthesis of oligosaccharides.
Topics: Animals; Escherichia coli; Galactose; Glycosyltransferases; Graft Rejection; Humans; Immobilization; Nickel; Nitrilotriacetic Acid; Oligosaccharides; Swine; Transplantation, Heterologous; Uridine Diphosphate | 2001 |
Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
Topics: Fluorescent Dyes; Histidine; Nickel; Nitrilotriacetic Acid; Proteins | 2001 |
Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypes.
Topics: Blotting, Western; Calcium; Cells, Cultured; Chromatography, Affinity; Dithiothreitol; Epitopes; Humans; Nickel; Nitrilotriacetic Acid; Photoaffinity Labels; Precipitin Tests; Radioligand Assay; Receptors, Adrenergic, alpha-1; Resins, Plant; Sulfhydryl Reagents; Urea | 2002 |
On-column tris(2-carboxyethyl)phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel-nitrilotriacetic acid Column.
Topics: DNA-Directed RNA Polymerases; Electrophoresis, Polyacrylamide Gel; Electrophoretic Mobility Shift Assay; Fluorescent Dyes; Maleimides; Models, Molecular; Nickel; Nitrilotriacetic Acid; Oxidation-Reduction; Peptide Fragments; Phosphines; Protein Conformation; Protein Folding; Protein Renaturation; Sensitivity and Specificity | 2002 |
Purification of histidine-tagged T4 RNA ligase from E. coli.
Topics: Bacteriophage T4; Base Sequence; Chromatography, Affinity; Escherichia coli; Histidine; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Recombinant Fusion Proteins; RNA Ligase (ATP); Sepharose; Viral Proteins | 2002 |
Buffered non-fermenter system for lab-scale production of secreted recombinant His-tagged proteins in Saccharomyces cerevisiae.
Topics: Buffers; Centrifugation; Chromatography, Affinity; Cloning, Molecular; Culture Media; Genes, Tumor Suppressor; Histidine; Humans; Hydrogen-Ion Concentration; Nickel; Nitrilotriacetic Acid; Oligopeptides; Peptides; Protein Biosynthesis; Proteins; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Serpins; Ultrafiltration | 2002 |
Cross-linking approach to affinity capture of protein complexes from chaotrope-solubilized cell lysates.
Topics: Affinity Labels; Calreticulin; Cell Line; Cross-Linking Reagents; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Histidine; Humans; Interleukin-12; Nickel; Nitrilotriacetic Acid; Peptides; Plasmids; Protein Denaturation; Proteins; Succinimides; Sulfhydryl Compounds; Urea | 2004 |
Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus.
Topics: Amino Acid Sequence; Chromatography, Affinity; Chromatography, Gel; Cloning, Molecular; Coronavirus 3C Proteases; Cysteine Endopeptidases; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Escherichia coli; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization; Viral Proteins | 2003 |
Finding needles in haystacks.
Topics: Binding Sites; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; Histidine; Ligands; Luminescent Proteins; Models, Biological; Mutation; Nickel; Nitrilotriacetic Acid; Oligonucleotides; Protein Binding; Protein Structure, Tertiary; Proteins | 2004 |
Insertion sequence 1 of muscle-specific calpain, p94, acts as an internal propeptide.
Topics: Amino Acid Sequence; Calcium; Calpain; Chelating Agents; Edetic Acid; Humans; Leucine; Leupeptins; Magnesium; Models, Molecular; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Peptide Fragments; Protease Inhibitors; Protein Precursors; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Deletion; Transposases | 2004 |
Orientated binding of photosynthetic reaction centers on gold using Ni-NTA self-assembled monolayers.
Topics: Adsorption; Atrazine; Biosensing Techniques; Coated Materials, Biocompatible; Crystallization; Electrochemistry; Equipment Design; Equipment Failure Analysis; Gold; Herbicides; Light; Macromolecular Substances; Materials Testing; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Photosynthetic Reaction Center Complex Proteins; Protein Binding; Protein Conformation | 2004 |
Comparative evaluation of two purification methods of anti-CD19-c-myc-His6-Cys scFv.
Topics: Antibodies, Monoclonal; Antigens, CD19; Blotting, Western; Chelating Agents; Chromatography, Affinity; Chromatography, Agarose; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Flow Cytometry; Histidine; Hybridomas; Hydrogen-Ion Concentration; Immunoglobulin Variable Region; Ion Exchange Resins; Mass Spectrometry; Molecular Weight; Nickel; Nitrilotriacetic Acid; Plasmids; Proto-Oncogene Proteins c-myc; Solubility; Temperature; Thiogalactosides | 2005 |
Controlling protein orientation at interfaces using histidine tags: an alternative to Ni/NTA.
Topics: Chelating Agents; Electrochemistry; Electrodes; Gold; Histidine; Models, Molecular; Nickel; Nitrilotriacetic Acid; Plastocyanin; Steroid 17-alpha-Hydroxylase; Surface Plasmon Resonance; Thioredoxins | 2005 |
Rapid evaluation of nickel binding properties of His-tagged lactate dehydrogenases using surface plasmon resonance.
Topics: Affinity Labels; Binding Sites; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Histidine; L-Lactate Dehydrogenase; Models, Molecular; Nickel; Nitrilotriacetic Acid; Protein Conformation; Surface Plasmon Resonance | 2005 |
Dense monolayers of metal-chelating ligands covalently attached to carbon electrodes electrochemically and their useful application in affinity binding of histidine-tagged proteins.
Topics: Binding Sites; Biosensing Techniques; Cations, Divalent; Chelating Agents; Copper; Diazonium Compounds; Electrochemistry; Electrodes; Graphite; Histidine; Imino Acids; Kinetics; Ligands; Metals; Microscopy, Fluorescence; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Spectrometry, X-Ray Emission; Surface Properties | 2005 |
Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein.
Topics: Animals; Blotting, Western; Carcinoma, Hepatocellular; Cattle; Cell Culture Techniques; Cell Line, Tumor; Cytoplasm; Electrophoresis, Gel, Two-Dimensional; Hepacivirus; Hepatocytes; Humans; Image Processing, Computer-Assisted; Immunohistochemistry; Keratins; Mass Spectrometry; Nickel; Nitrilotriacetic Acid; Precipitin Tests; Proteins; Proteomics; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin; Vimentin; Viral Core Proteins | 2005 |
Proteomic identification of ubiquitinated proteins from human cells expressing His-tagged ubiquitin.
Topics: Amino Acid Sequence; Blotting, Western; Cell Line; Chromatography, Affinity; Eukaryotic Cells; Green Fluorescent Proteins; Histidine; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Proliferating Cell Nuclear Antigen; Proteins; Proteomics; Substrate Specificity; Transfection; Transgenes; Ubiquitin | 2005 |
High-affinity adaptors for switchable recognition of histidine-tagged proteins.
Topics: Calorimetry; Chelating Agents; Fluorescein; Histidine; Humans; Kinetics; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Protein Binding; Receptor, Interferon alpha-beta; Receptors, Interferon; Thermodynamics | 2005 |
Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged Ni-NTA Nanogold labeling.
Topics: Bacterial Proteins; Immunohistochemistry; Macromolecular Substances; Nickel; Nitrilotriacetic Acid; Phosphoproteins; Photosystem II Protein Complex; Protein Structure, Quaternary; Protein Subunits; Synechocystis; Thylakoids | 2005 |
Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate.
Topics: Fluoresceins; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Photochemistry; Proteins; Transfection | 2006 |
Differential protein assembly on micropatterned surfaces with tailored molecular and surface multivalency.
Topics: Chelating Agents; Histidine; Imidazoles; Interferon-alpha; Ligands; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Protein Array Analysis; Protein Binding; Proteins; Receptor, Interferon alpha-beta; Receptors, Interferon; Recombinant Fusion Proteins; Sulfhydryl Compounds; Surface Properties | 2006 |
Preparation of hepatitis C virus structural and non-structural protein fragments and studies of their immunogenicity.
Topics: Animals; Antigen-Antibody Reactions; Cell Proliferation; Chromatography, Affinity; Cloning, Molecular; Dose-Response Relationship, Immunologic; Female; Hepatitis C Antibodies; Hepatitis C Antigens; Immunization; Mice; Mice, Inbred BALB C; Nickel; Nitrilotriacetic Acid; Peptide Fragments; Peptides; Recombinant Proteins; Sensitivity and Specificity; Spleen; Viral Core Proteins; Viral Nonstructural Proteins; Viral Proteins | 2006 |
Cyanobacterial small chlorophyll-binding protein ScpD (HliB) is located on the periphery of photosystem II in the vicinity of PsbH and CP47 subunits.
Topics: Bacterial Proteins; Crystallography, X-Ray; Cyanobacteria; Dimerization; Epitopes; Histidine; Immunohistochemistry; Light-Harvesting Protein Complexes; Models, Molecular; Mutation; Nickel; Nitrilotriacetic Acid; Photosystem II Protein Complex; Protein Subunits; Synechocystis; Thylakoids | 2006 |
Mutagenesis of hydrogenase accessory genes of Synechocystis sp. PCC 6803. Additional homologues of hypA and hypB are not active in hydrogenase maturation.
Topics: Base Sequence; Hydrogenase; Molecular Sequence Data; Multigene Family; Mutagenesis; Nickel; Nitrilotriacetic Acid; Synechocystis | 2006 |
Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol).
Topics: Adsorption; Biosensing Techniques; Histidine; In Vitro Techniques; Lipid Bilayers; Molecular Structure; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Proteins; Silicon Dioxide; Surface Properties | 2007 |
Creating nanopatterns of His-tagged proteins on surfaces by nanoimprint lithography using specific NiNTA-histidine interactions.
Topics: Coated Materials, Biocompatible; Crystallization; Histidine; Macromolecular Substances; Materials Testing; Molecular Conformation; Nanostructures; Nanotechnology; Nickel; Nitrilotriacetic Acid; Particle Size; Proteins; Staining and Labeling; Surface Properties | 2007 |
Determination of the rate constants for the FK506 binding protein/rapamycin interaction using surface plasmon resonance: an alternative sensor surface for Ni2+-nitrilotriacetic acid immobilization of His-tagged proteins.
Topics: Histidine; Humans; Kinetics; Nickel; Nitrilotriacetic Acid; Recombinant Proteins; Sirolimus; Surface Plasmon Resonance; Tacrolimus Binding Proteins | 2007 |
High-capacity purification of His-tagged proteins by affinity membranes containing functionalized polymer brushes.
Topics: Adsorption; Aluminum; Gold; Histidine; Membranes, Artificial; Methacrylates; Models, Chemical; Nickel; Nitrilotriacetic Acid; Polyhydroxyethyl Methacrylate; Polymers; Protein Engineering; Proteins; Silver Staining | 2007 |
Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix.
Topics: Aldehyde-Lyases; Benzoin; Bioreactors; Catalysis; Enzymes, Immobilized; Escherichia coli; Esters; Hydrolysis; Lysine; Nickel; Nitrilotriacetic Acid; Povidone; Stereoisomerism; Tyrosine | 2007 |
Anchoring of histidine-tagged proteins to molecular printboards: self-assembly, thermodynamic modeling, and patterning.
Topics: Adsorption; beta-Cyclodextrins; Binding Sites; Carrier Proteins; Histidine; Luminescent Proteins; Maltose-Binding Proteins; Models, Chemical; Molecular Conformation; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Proteasome Endopeptidase Complex; Surface Plasmon Resonance; Surface Properties; Thermodynamics; Time Factors | 2008 |
A polymeric molecular "handle" for multiple AFM-based single-molecule force measurements.
Topics: Chelating Agents; Histidine; Microscopy, Atomic Force; Nanotechnology; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds | 2008 |
Metal-chelate affinity chromatography.
Topics: Animals; Base Sequence; Chelating Agents; Chromatography, Affinity; Cloning, Molecular; Dialysis; Genetic Vectors; Histidine; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Protein Renaturation; Recombinant Fusion Proteins | 2001 |
Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy.
Topics: Adsorption; Animals; Cell Extracts; Chemistry Techniques, Analytical; Cryoelectron Microscopy; Histidine; Lipid Metabolism; Multiprotein Complexes; Nickel; Nitrilotriacetic Acid; Oligopeptides; Receptors, Transferrin; Ribosomal Protein L3; Ribosomal Proteins; Ribosomes; Transferrin | 2008 |
Exploiting the nitrilotriacetic acid moiety for biolabeling with ultrastable perylene dyes.
Topics: Adenosine Triphosphatases; Binding Sites; Escherichia coli; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Nickel; Nitrilotriacetic Acid; Perylene; Photons; Solubility; Water | 2008 |
Ni-nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells.
Topics: Cell Line; Cell Survival; Fluorescent Dyes; Histidine; Humans; Nickel; Nitrilotriacetic Acid; Proteins; Quantum Dots; Staining and Labeling | 2008 |
Metal-chelate affinity chromatography.
Topics: Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Genetic Vectors; Histidine; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Protein Denaturation; Protein Renaturation; Recombinant Fusion Proteins; Resins, Synthetic | 2001 |
Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots.
Topics: Blotting, Western; Cadmium Compounds; Nickel; Nitrilotriacetic Acid; Proteins; Quantum Dots; Reproducibility of Results; Selenium Compounds; Sulfides; Zinc Compounds | 2008 |
Protein immobilization without purification via dip-pen nanolithography.
Topics: Adsorption; Escherichia coli Proteins; Green Fluorescent Proteins; Ligands; Microscopy, Atomic Force; Nanotechnology; Nickel; Nitrilotriacetic Acid; Printing; Protein Array Analysis; Proteins; Surface Properties | 2008 |
Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.
Topics: Animals; Cadmium Compounds; Carboxylic Acids; Catalytic Domain; Fluorescent Dyes; Glutathione Transferase; Histidine; Models, Molecular; Molecular Conformation; Nickel; Nitrilotriacetic Acid; Protein Binding; Quantum Dots; Schistosoma; Selenium Compounds; Substrate Specificity; Sulfides; Surface Properties; Zinc Compounds | 2008 |
Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging.
Topics: Absorption; Amino Acid Sequence; Animals; Case-Control Studies; Cell Line, Tumor; Cell Survival; Cricetinae; Ferrosoferric Oxide; Humans; Magnetic Resonance Imaging; Male; Materials Testing; Mice; Mouth Neoplasms; Nanoparticles; Nickel; Nitrilotriacetic Acid; Oligopeptides; Peptides, Cyclic; Tissue Distribution | 2008 |
Analysis of nickel-binding peptides in a human hepidermoid cancer cell line by Ni-NTA affinity chromatography and mass spectrometry.
Topics: Actins; Amino Acid Sequence; Carcinoma, Squamous Cell; Cell Line, Tumor; Chromatography, Affinity; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Tandem Mass Spectrometry; Trypsin | 2008 |
Nickel-ion-mediated control of the stoichiometry of his-tagged protein/nanoparticle interactions.
Topics: Electrophoresis, Agar Gel; Fluorescence; Green Fluorescent Proteins; Histidine; Ions; Kinetics; Ligands; Nanoparticles; Nickel; Nitrilotriacetic Acid; Oligopeptides; Sulfhydryl Compounds; Titrimetry | 2009 |
Amphiphilic diblock copolymers for molecular recognition: metal-nitrilotriacetic acid functionalized vesicles.
Topics: Butadienes; Copper; Molecular Structure; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Polyethylene; Surface Properties | 2009 |
High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates.
Topics: Biocompatible Materials; Cadmium Compounds; Cell Adhesion; Green Fluorescent Proteins; HeLa Cells; Histidine; Humans; Microscopy, Fluorescence; Nanotechnology; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Quantum Dots; Receptors, Interferon; Selenium Compounds; Sulfides; Zinc Compounds | 2009 |
A facile method for reversibly linking a recombinant protein to DNA.
Topics: DNA; Histidine; Nickel; Nitrilotriacetic Acid; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Oligopeptides; Protein Binding; Recombinant Proteins | 2009 |
Evaluation of immobilized metal-ion affinity chromatography (IMAC) as a technique for IgG(1) monoclonal antibodies purification: the effect of chelating ligand and support.
Topics: Adsorption; Antibodies, Monoclonal; Aspartic Acid; Chelating Agents; Chromatography, Affinity; Ethylenediamines; Imino Acids; Immunoglobulin G; Ligands; Membranes, Artificial; Metals; Nickel; Nitrilotriacetic Acid; Surface Properties; Zinc | 2010 |
Reassembling biological machinery in vitro.
Topics: Adenosine Triphosphate; Animals; Enzymes, Immobilized; Glucose; Glucose-6-Phosphate Isomerase; Hexokinase; Mice; Nickel; Nitrilotriacetic Acid | 2009 |
Sequential reactions of surface- tethered glycolytic enzymes.
Topics: Adenosine Triphosphate; Animals; Enzymes, Immobilized; Glucose; Glucose-6-Phosphate Isomerase; Hexokinase; Mice; Nickel; Nitrilotriacetic Acid; Recombinant Proteins | 2009 |
Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(II)-NTA probe.
Topics: Binding Sites; Coumarins; Fluorescent Dyes; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Staining and Labeling | 2009 |
Insight into the complexation mode of bis(nitrilotriacetic acid) (NTA) ligands with Ni(2+) involved in the labeling of histidine-tagged proteins.
Topics: Calorimetry; Fluorescence Polarization; Histidine; Ligands; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Staining and Labeling | 2009 |
Bisphosphonate adaptors for specific protein binding on zirconium phosphonate-based microarrays.
Topics: Archaeal Proteins; Binding Sites; Enzymes, Immobilized; Histidine; Membranes, Artificial; Muramidase; Nickel; Nitrilotriacetic Acid; Organophosphonates; Particle Size; Protein Array Analysis; Recombinant Proteins; Sulfolobus acidocaldarius; Surface Properties; Zirconium | 2009 |
Protein purification with polymeric affinity membranes containing functionalized poly(acid) brushes.
Topics: Binding Sites; Carrier Proteins; Chromatography, Affinity; Egg White; Histidine; Humans; Membranes, Artificial; Muramidase; Nickel; Nitrilotriacetic Acid; Nylons; Polymers; Protein Binding; Spectroscopy, Fourier Transform Infrared; Ubiquitin | 2010 |
Colloidal probe microscopy of membrane-membrane interactions: from ligand-receptor recognition to fusion events.
Topics: Cell Membrane; Ligands; Lipid Bilayers; Lipopeptides; Liposomes; Membrane Fusion; Membrane Lipids; Microscopy, Scanning Probe; Nickel; Nitrilotriacetic Acid; Phospholipids; Phosphorylcholine; Silicon | 2010 |
Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires.
Topics: Biosensing Techniques; Histidine; Immobilized Proteins; Nanowires; Nickel; Nitrilotriacetic Acid; Proteins; Silicon | 2010 |
Complex surface concentration gradients by stenciled "electro click chemistry".
Topics: Alkynes; Bridged Bicyclo Compounds, Heterocyclic; Click Chemistry; Copper; Electric Conductivity; Electrodes; Immobilized Proteins; Nickel; Nitrilotriacetic Acid; Oligopeptides; Photoelectron Spectroscopy; Polymers; Surface Properties | 2010 |
Ultra-sensitive detection of prion protein with a long range resonance energy transfer strategy.
Topics: Cell Line, Tumor; Fluorescence Resonance Energy Transfer; Gold; Humans; Metal Nanoparticles; Nickel; Nitrilotriacetic Acid; Prions; Quantum Dots | 2010 |
A practical guide to the use of monolayer purification and affinity grids.
Topics: Cryoelectron Microscopy; Crystallography; Lipids; Nickel; Nitrilotriacetic Acid; Proteins | 2010 |
Controlled stoichiometric synthesis of DNA-quantum dot conjugates using Ni-mediated coordination chemistry.
Topics: Fluorescence Resonance Energy Transfer; Histidine; Nickel; Nitrilotriacetic Acid; Oligonucleotides; Quantum Dots | 2010 |
Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle.
Topics: Biocatalysis; Enzymes, Immobilized; Epoxide Hydrolases; Magnetics; Models, Molecular; Nanoparticles; Nickel; Nitrilotriacetic Acid; Protein Conformation; Solanum tuberosum | 2011 |
Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells.
Topics: CD8-Positive T-Lymphocytes; Histidine; HLA-A2 Antigen; Humans; MART-1 Antigen; Nickel; Nitrilotriacetic Acid; Peptides; Staining and Labeling | 2011 |
Development of a histidine-targeted spectrophotometric sensor using Ni(II)NTA-functionalized Au and Ag nanoparticles.
Topics: Amino Acid Sequence; Antigens, Protozoan; Colloids; Gold; Histidine; Humans; Hydrogen-Ion Concentration; Malaria, Falciparum; Metal Nanoparticles; Microscopy, Electron, Transmission; Molecular Mimicry; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Peptides; Plasmodium falciparum; Protozoan Proteins; Reagent Kits, Diagnostic; Silver; Spectrophotometry | 2011 |
In vitro and in vivo assessment of targeting lipid-based nanoparticles to the epidermal growth factor-receptor (EGFR) using a novel Heptameric ZEGFR domain.
Topics: Animals; Cell Line, Tumor; Drug Carriers; ErbB Receptors; Humans; Metal Nanoparticles; Mice; Mice, Nude; Neoplasms; Nickel; Nitrilotriacetic Acid; Polyethylene Glycols; Protein Structure, Tertiary; Succinimides; Surface-Active Agents; Tissue Distribution; Triglycerides | 2012 |
Selective two-step labeling of proteins with an off/on fluorescent probe.
Topics: Binding Sites; Fluorescence; Fluorescent Dyes; Green Fluorescent Proteins; Molecular Structure; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Peptides; Proteins | 2011 |
Development of a novel optical biosensor for detection of organophosphorus pesticides based on methyl parathion hydrolase immobilized by metal-chelate affinity.
Topics: Biosensing Techniques; Chelating Agents; Chromatography, Affinity; Enzymes, Immobilized; Histidine; Metals; Methyl Parathion; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organophosphorus Compounds; Pesticides; Phosphoric Monoester Hydrolases; Recombinant Proteins | 2012 |
Kinetic studies of Ni organic complexes using diffusive gradients in thin films (DGT) with double binding layers and a dynamic numerical model.
Topics: Benzopyrans; Chelating Agents; Diffusion; Humic Substances; Kinetics; Models, Theoretical; Nickel; Nitrilotriacetic Acid; Water Pollutants, Chemical | 2013 |
pH responsive PEGylation through metal affinity for gene delivery mediated by histidine-grafted polyethylenimine.
Topics: Animals; Biocompatible Materials; Cattle; Chelating Agents; CHO Cells; Coated Materials, Biocompatible; Cricetinae; Cricetulus; Gene Transfer Techniques; Histidine; Hydrogen-Ion Concentration; Materials Testing; Nickel; Nitrilotriacetic Acid; Plasmids; Polyethylene Glycols; Polyethyleneimine; Transfection; Zinc | 2013 |
Silicatein conjugation inside nanoconfined geometries through immobilized NTA-Ni(II) chelates.
Topics: Cathepsins; Chelating Agents; Molecular Structure; Nanoparticles; Nickel; Nitrilotriacetic Acid; Organometallic Compounds | 2013 |
Ni(II)-NTA modified poly(ethylene imine) glycopolymers: physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides.
Topics: Amino Acid Sequence; Cell Death; Cell Differentiation; Chemical Phenomena; Dendritic Cells; HIV; Humans; Imines; Light; Maltose; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Peptides; Polyethylenes; Scattering, Radiation | 2013 |
Identification of metal ion binding peptides containing unnatural amino acids by phage display.
Topics: 2,2'-Dipyridyl; Amino Acid Sequence; Amino Acids; Hydrogen-Ion Concentration; Ions; Kinetics; Metals; Nickel; Nitrilotriacetic Acid; Peptide Library; Peptides, Cyclic; Protein Binding | 2013 |
Immobilization of enzymes on magnetic beads through affinity interactions.
Topics: Chelating Agents; Enzymes, Immobilized; Histidine; Magnetic Phenomena; Magnetite Nanoparticles; Nickel; Nitrilotriacetic Acid; Oligopeptides; Plant Lectins; Protein Binding; Streptavidin | 2013 |
Vertical nanowire arrays as a versatile platform for protein detection and analysis.
Topics: Animals; Arsenicals; Biotin; Cattle; Fluorescent Dyes; Immobilized Proteins; Immunoassay; Indium; Maleimides; Nanowires; Nickel; Nitrilotriacetic Acid; Protein Array Analysis; Proteins; Serum Albumin; Streptavidin | 2013 |
An electrochemical peptide-based Ara h 2 antibody sensor fabricated on a nickel(II)-nitriloacetic acid self-assembled monolayer using a His-tagged peptide.
Topics: Antibodies; Biosensing Techniques; Electrochemical Techniques; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides | 2014 |
Hybridization of an Aβ-specific antibody fragment with aminopyrazole-based β-sheet ligands displays striking enhancement of target affinity.
Topics: Alzheimer Disease; Amyloid beta-Peptides; Catalysis; Enzyme-Linked Immunosorbent Assay; Epitopes; Histidine; Humans; Hydrogen; Hydrogen-Ion Concentration; Immunoglobulin Fragments; Immunotherapy; Ligands; Magnetic Resonance Spectroscopy; Nickel; Nitrilotriacetic Acid; Peptide Fragments; Protein Structure, Secondary; Protein Structure, Tertiary; Pyrazoles; Recombinant Proteins; Single-Chain Antibodies | 2015 |
In vitro systems for the study of microtubule-based cell polarity in fission yeast.
Topics: Actin Cytoskeleton; Cell Membrane; Cell Polarity; Chromatography, Affinity; Histidine; Microfluidics; Microtubule-Associated Proteins; Microtubules; Nickel; Nitrilotriacetic Acid; Oligopeptides; Phosphoprotein Phosphatases; Phosphorylation; Protein Binding; Protein Kinases; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Spindle Pole Bodies | 2015 |
Assay of Copper Transfer and Binding to P1B-ATPases.
Topics: Adenosine Triphosphatases; Binding, Competitive; Biological Transport; Copper; Molecular Chaperones; Nickel; Nitrilotriacetic Acid; Protein Binding; Quinolines; Solubility | 2016 |
Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer.
Topics: Actin Cytoskeleton; Actomyosin; Adenosine Triphosphate; Animals; Bacterial Proteins; Cell Membrane; Cell Polarity; Chelating Agents; Chickens; Cytoskeletal Proteins; In Vitro Techniques; Lipid Bilayers; Luminescent Proteins; Membrane Proteins; Microscopy, Electron; Models, Biological; Nickel; Nitrilotriacetic Acid; Phosphatidylcholines; Phosphatidylethanolamines; Protein Binding; Recombinant Fusion Proteins; Surface Properties | 2016 |
Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins.
Topics: Capsid Proteins; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Peptides | 2016 |
Simultaneous determination of thermodynamic and kinetic parameters of aminopolycarbonate complexes of cobalt(II) and nickel(II) based on isothermal titration calorimetry data.
Topics: Calorimetry; Cobalt; Imino Acids; Kinetics; Nickel; Nitrilotriacetic Acid; Thermodynamics | 2017 |
Label-free histamine detection with nanofluidic diodes through metal ion displacement mechanism.
Topics: Carboxylic Acids; Chelating Agents; Electric Conductivity; Electrodes; Esters; Histamine; Hydrolysis; Ions; Metals; Models, Theoretical; Nanopores; Nanotechnology; Neurotransmitter Agents; Nickel; Nitrilotriacetic Acid; Polymers; Surface Properties | 2017 |
Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags.
Topics: Animals; Histidine; Immobilized Proteins; Luminescent Proteins; Molecular Dynamics Simulation; Nickel; Nitrilotriacetic Acid; Oligopeptides; Red Fluorescent Protein; Sea Anemones; Surface Properties | 2017 |
Orthogonal supramolecular protein assembly on patterned bifunctional surfaces.
Topics: Adamantane; beta-Cyclodextrins; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Surface Properties | 2018 |
Effect of Bacillus subtilis and NTA-APG on pyrene dissipation in phytoremediation of nickel co-contaminated wetlands by Scirpus triqueter.
Topics: Bacillus subtilis; Biodegradation, Environmental; Biomass; Cyperaceae; Glucosides; Nickel; Nitrilotriacetic Acid; Pyrenes; Soil; Soil Microbiology; Soil Pollutants; Wetlands | 2018 |
Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template.
Topics: Green Fluorescent Proteins; Histidine; Immobilized Proteins; Nanocapsules; Nickel; Nitrilotriacetic Acid; Polymers; Surface Properties | 2018 |
Covalent affixation of histidine-tagged proteins tethered onto Ni-nitrilotriacetic acid sensors for enhanced surface plasmon resonance detection of small molecule drugs and kinetic studies of antibody/antigen interactions.
Topics: Antigen-Antibody Reactions; Antigens; Carbonic Anhydrase II; Dextrans; Histidine; Humans; Immunoglobulin Fc Fragments; Kinetics; Limit of Detection; Nickel; Nitrilotriacetic Acid; Pharmaceutical Preparations; Sulfonamides; Surface Plasmon Resonance | 2019 |
Green Fluorescent Probe for Imaging His
Topics: Cell Survival; Escherichia coli; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Intracellular Space; Nickel; Nitrilotriacetic Acid; Optical Imaging; Proteins; Solubility; Xeroderma Pigmentosum Group A Protein | 2019 |
Protein encapsulation in the hollow space of hemocyanin crystals containing a covalently conjugated ligand.
Topics: Animals; Biotin; Chelating Agents; Crystallization; Crystallography, X-Ray; Decapodiformes; Hemocyanins; Immobilized Proteins; Ligands; Models, Molecular; Nickel; Nitrilotriacetic Acid; Protein Multimerization; Streptavidin | 2019 |
Bottom-up assembly of a bilayer structure of icosahedral viral nanoparticles.
Topics: Capsid Proteins; Gold; Microscopy, Atomic Force; Nanoparticles; Nanostructures; Nickel; Nitrilotriacetic Acid; Tombusvirus | 2020 |
Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent.
Topics: HEK293 Cells; Histidine; Humans; Indicators and Reagents; Lysine; Models, Molecular; Molecular Probes; Molecular Structure; Nickel; Nitrilotriacetic Acid; Proteins; Staining and Labeling; Sulfonamides | 2021 |
Tyrosine-Based Dual-Functional Interface for Trapping and On-Site Photo-Induced Covalent Immobilization of Proteins.
Topics: Histidine; Indicators and Reagents; Nickel; Nitrilotriacetic Acid; Tyrosine | 2022 |
Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.
Topics: Fluorescence; Histidine; Magnetite Nanoparticles; Nickel; Nitrilotriacetic Acid; Peptide Hydrolases; Peptides | 2023 |