nisin-z and subtilin

nisin-z has been researched along with subtilin* in 1 studies

Other Studies

1 other study(ies) available for nisin-z and subtilin

ArticleYear
Biosynthesis and secretion of a precursor of nisin Z by Lactococcus lactis, directed by the leader peptide of the homologous lantibiotic subtilin from Bacillus subtilis.
    FEBS letters, 1993, Sep-06, Volume: 330, Issue:1

    The DNA sequence encoding the leader peptide of the lantibiotic subtilin from Bacillus subtilis was fused to the sequence encoding pronisin Z, and this hybrid gene was expressed in a Lactococcus lactis strain that produces nisin A. This strain simultaneously secreted nisin A and a protein of approximately 6 kDa. Amino acid sequencing of the purified 6 kDa protein and structural analysis of its main tryptic fragment by two-dimensional 1H-NMR showed that it consists of the unmodified leader peptide of subtilin, without the N-terminal methionine residue, linked to a fully matured nisin Z part. The hybrid protein and its main tryptic fragment [ITPQ]-nisin Z, showed at least 200-fold lower antimicrobial activities than nisin Z against three different indicator strains.

    Topics: Amino Acid Sequence; Anti-Bacterial Agents; Bacillus subtilis; Bacterial Proteins; Bacteriocins; Base Sequence; Cloning, Molecular; DNA, Bacterial; Lactococcus lactis; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Nisin; Peptides; Peptides, Cyclic; Protein Precursors; Protein Sorting Signals; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Trypsin

1993