nigranoic-acid has been researched along with corosolic-acid* in 1 studies
1 other study(ies) available for nigranoic-acid and corosolic-acid
| Article | Year |
|---|---|
Purification of two triterpenoids from Schisandra chinensis by macroporous resin combined with high-speed counter-current chromatography.
A method for preparative purification of corosolic acid and nigranoic acid from Schisandra chinensis (SC) was established using a combination of macroporous absorption resin column separation and high-speed counter-current chromatography (HSCCC). The crude extracts obtained from SC using 70% ethanol were separated on a macroporous resin column and then eluted with a graded ethanol series. The 70% ethanol fraction was used as the sample for separation of the two triterpenoids by HSCCC. The two-phase solvent system used for HSCCC separation was chloroform-n-butanol-methanol-water (10:0.5:7:4, v/v/v/v). The upper phase was used as the stationary phase of HSCCC. Corosolic acid (16.4 mg) of 96.3% purity and nigranoic acid (9.5 mg) of 98.9% purity were obtained in a one-step HSCCC separation from 100 mg of the sample. The structures of corosolic acid and nigranoic acid were identified by (1)H-nuclear magnetic resonance (NMR) and (13)C-NMR. Topics: 1-Butanol; Chloroform; Chromatography, High Pressure Liquid; Countercurrent Distribution; Data Interpretation, Statistical; Equipment Design; Magnetic Resonance Spectroscopy; Methanol; Molecular Structure; Reproducibility of Results; Resins, Synthetic; Schisandra; Solvents; Triterpenes | 2014 |