nickel nitrilotriacetic acid has been researched along with nitrilotriacetic acid in 103 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 0 (0.00) | 18.7374 |
| 1990's | 21 (20.39) | 18.2507 |
| 2000's | 40 (38.83) | 29.6817 |
| 2010's | 39 (37.86) | 24.3611 |
| 2020's | 3 (2.91) | 2.80 |
| Authors | Studies |
|---|---|
| Gennis, RB; Mitchell, DM | 1 |
| Island, MD; Maneval, D; Mobley, HL; Sriwanthana, B | 1 |
| Funk, CD; Marnett, LJ; Reddy, RG; Yamamoto, S; Yoshimoto, T | 1 |
| Crowe, J; Masone, BS; Ribbe, J | 2 |
| Dougherty, JP; Dunn, KE; Gibbs, CS; Paborsky, LR | 1 |
| Akizawa, T; Imai, K; Ito, Y; Itoh, M; Masuda, K; Okada, Y; Sato, H; Seiki, M; Yoshioka, M | 1 |
| Kaback, HR; Prive, GG | 1 |
| Ji, Z; Miller, LJ; Pinon, DI | 1 |
| Brown, KG; Grant, KM; McCready, BP; Mottram, JC | 1 |
| Brumfield, S; Filichkin, SA; Filichkin, TP; Young, MJ | 1 |
| Hidaka, Y; Inouye, M; Park, H | 1 |
| Deutscher, SL; Komissarov, AA; Marchbank, MT | 1 |
| Homma, T; Ito, K; Yoshihisa, T | 1 |
| Chirgwin, JM; Sachdev, D | 1 |
| Arimura, Y; Fujii, A; Hashimoto, Y; Ishida-Okawara, A; Ito, M; Mine, E; Mizuno, S; Nagasawa, T; Ohashi, YY; Suzuki, K; Tanokura, M; Tomizawa, K; Yamagoe, S; Yamamoto, T | 1 |
| Arthur, TM; Burgess, RR | 1 |
| Atkins, WM; Chen, JP; Chin, WC; Dabrowski, MJ; Shi, H | 1 |
| Freimuth, P; Hainfeld, JF; Halsey, CM; Liu, W; Powell, RD | 1 |
| Beresten, SF; Ellis, NA; Naureckiene, S; Stan, R; van Brabant, AJ; Ye, T | 1 |
| Cocca, BA; Radic, MZ; Seal, SN | 1 |
| Bennett, TA; Lynam, E; Neldon, D; Prossnitz, E; Sklar, LA; Vilven, J | 1 |
| Hasegawa, M; Miyake, J; Nakamura, C; Yasuda, Y | 1 |
| Callis, J; Colón, E; Dahmus, ME; Ling, R | 1 |
| Baumann, CG; Ferguson, AL; Hoggett, JG; Hughes, AD; Scott, DJ; Tufail, U | 1 |
| Bornhorst, JA; Falke, JJ | 1 |
| Nickel, W; Wieland, FT | 1 |
| Barlowe, C; Belden, WJ | 1 |
| Curiel, DT; Pereboev, A; Pereboeva, L | 1 |
| Feltens, R; Hartmann, RK; Pfeiffer, T; Rox, C | 1 |
| Lauer, SA; Nolan, JP | 1 |
| Blattler, C; Braun, JE; Kay, JG; Swayne, LA | 1 |
| Kohli, BM; Ostermeier, C | 1 |
| Mori, S; Nishibori, M; Okamoto, M; Takahashi, HK; Yamaoka, K | 1 |
| Kennan, AJ; Schnarr, NA | 1 |
| De Francesco, M; Di Meo, C; Maly, J; Masci, A; Masojidek, J; Pilloton, R; Sugiura, M; Volpe, A | 1 |
| Lebedev, N; Shashidhar, R; Trammell, SA; Wang, L; Zullo, JM | 1 |
| Cohen, E; Ebel, C; Goldshleger, R; Karlish, SJ; le Maire, M; Shainskaya, A; Tal, DM | 1 |
| Lata, S; Piehler, J | 1 |
| Fahr, A; Kontermann, RE; Müller, D; Rüger, R | 2 |
| Borrebaeck, CA; Ingvarsson, J; Lindstedt, M; Wingren, C | 1 |
| Batt, CA; Coates, GW; Kim, YR; Mark, SS; Ober, CK; Paik, HJ; Ryan, TE | 1 |
| Bauersachs, S; Blum, H; Donzeau, M; Nagel, W; Reichelt, P; Röhnisch, T | 1 |
| Coorssen, JR; Howell, JM; Turner, RJ; Winstone, TL | 1 |
| Champeil, P; Falson, P; Jaxel, C; le Maire, M; Lenoir, G; Picard, M | 1 |
| He, M; Khan, F; Taussig, MJ | 1 |
| Campbell-Valois, FX; Michnick, SW | 1 |
| Higashiya, S; Ngo, SC; Topilina, NI; Welch, JT; Zagorevskii, D | 1 |
| Kennan, AJ; Ryan, SJ | 1 |
| Jeyakumar, M; Katzenellenbogen, JA; Kim, SH | 1 |
| Briñas, RP; Hainfeld, JF; Hu, M; Lymar, ES; Qian, L | 1 |
| Chen, XL; Gong, CC; He, PX; Liu, YL; Wang, GL; Wang, WL; Zhang, CY; Zheng, SN | 1 |
| Gopala Krishna, A; Gummadi, SN; Sahu, SK | 1 |
| Kühlbrandt, W; Meisinger, C; Model, K | 1 |
| Hammond, RW; Kovalskaya, N | 1 |
| Baksh, MM; Brown, S; Finn, MG; Udit, AK | 1 |
| Arcari, P; Chambery, A; Di Maro, A; Lamberti, A; Longo, O; Masullo, M; Parente, A; Sanges, C | 1 |
| Ge, P; Katzenellenbogen, JA; Kim, SH | 1 |
| He, H; Ma, W; Wang, C; Yang, H; Yang, S; Zhang, H | 1 |
| Nakayama, T; Namura, M; Noji, H; Tabata, KV; Yokokawa, R | 1 |
| Choi, Y; Grailhe, R; Hong, S; Kim, H; Kim, K; Kwon, D; Park, HY; Ryu, J; Song, R | 1 |
| Bowman, WS; Fried, MG; Hellman, LM; Whiteheart, SW; Zhan, X; Zhao, C | 1 |
| Byrne, NA; Walker, KD; Wijeratne, S | 1 |
| Esfandiar, S; Hashemi-Najafabadi, S; Pourpak, Z; Sarrafzadeh, SA; Shojaosadati, SA | 1 |
| Davis, ME; García, AJ; Murthy, N; Phelps, EA; Sy, JC | 1 |
| Cao, L; Huang, Z; Platt, V; Riviere, K; Szoka, FC; Tiffany, M | 1 |
| Dobrowolski, A; Fusetti, F; Lolkema, JS | 1 |
| Hanson, PI; Merrill, SA | 1 |
| Jiang, S; Pun, SH; Wang, CH | 1 |
| Barron-Casella, E; Casella, JF; Dulloor, P; Everett, A; Fu, Z; Savage, W; Van Eyk, JE; Williams, LM; Yen, T | 1 |
| Swartz, JR; Yang, WC | 1 |
| Gonchar, MV; Horak, D; Potocka, N; Rzhepetskyy, YA; Stasyk, OV; Zakalska, OM; Zakalskiy, AE | 1 |
| Baker, GL; Bhattacharjee, S; Bruening, ML; Dong, J; Geiger, JH; Hovde, S; Ma, Y | 1 |
| Carrión-Vázquez, M; Hervás, R; Oroz, J; Valbuena, A | 1 |
| Davies, AA; Ulrich, HD | 1 |
| Bostina, M; Filman, DJ; Hogle, JM; Levy, HC; Strauss, M | 1 |
| Križaj, I; Leonardi, A; Maček, P; Mikelj, M; Ota, K; Papler, T | 1 |
| Guo, M; Huang, Y; Lei, C; Nie, Z; Wang, M; Yao, S | 1 |
| Afonso, CL; Brindley, MA; Byrd-Leotis, LA; Campbell, P; Chen, X; Guerrero-Ferreira, RC; Holl, JM; Ke, Z; Kelly, DF; Kiss, G; Plemper, RK; Spearman, PW; Steel, J; Steinhauer, DA; Wright, ER | 1 |
| Kirk, WR | 1 |
| Brenke, JK; Gopalakrishnan, J; Gul, S; Hadian, K; Low, T; Reinshagen, J; Rothenaigner, I; Salmina, E; Schorpp, K; Tetko, IV | 1 |
| Oberoi-Khanuja, TK; Rajalingam, K | 1 |
| Dogterom, M; Dries, R; Piehler, J; Taberner, N; Weber, G; You, C | 1 |
| Alsteens, D; Hilbert, M; Müller, DJ; Pfreundschuh, M; Steinmetz, MO | 1 |
| Irudayaraj, J; Manorama, SV; Narsireddy, A; Rao, NM; Vijayashree, K | 1 |
| Bang, SH; Hong, SW; Hyun, YJ; Kim, DH; Shim, J | 1 |
| Gulka, CP; Swartz, JD; Wright, DW | 1 |
| Bartsch, JW; Koller, G; Moss, ML; Rakow, S; Rasmussen, FH; Schlomann, U | 1 |
| Cherkouk, C; Haase-Kohn, C; Keller, A; Laube, M; Lenk, J; Neuber, C; Ou, X; Pietzsch, J; Rebohle, L; Skorupa, W | 1 |
| Brett, TJ; Kober, DL; Yurtsever, Z | 1 |
| Braunger, J; Cordes, A; Gerdes, B; Gerke, V; Grill, D; Kramer, C; Mey, I; Schäfer, J; Shabardina, V; Steinem, C | 1 |
| Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T | 1 |
| Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Ohoka, N; Okitsu, K; Shibata, N; Yamazaki, N | 1 |
| Cabanas-Danés, J; Huskens, J; Jonkheijm, P; Subramaniam, V; Wasserberg, D | 1 |
| Huang, M; Kaneko, T; Li, L; Li, SSC; Voss, C; Wang, R; Xia, J; Zhang, L | 1 |
| Choo, J; Lee, EK; Lee, S; Park, JP; Yun, S | 1 |
| Himeno, T; Hirata, S; Horiuchi, J; Ishimatsu, R; Nakano, K; Yamanaka, M | 1 |
| Burger, J; Herz, T; Kilb, N; Meyer, PA; Roth, G; Woehrle, J | 1 |
| Kokhan, O; Marzolf, DR | 1 |
| Brüchert, S; Gatterdam, K; Joest, EF; Tampé, R | 1 |
| Cao, Y; Fang, X; Gao, T; Li, W; Luo, Y; Pei, R; Ullah, S; Yang, L | 1 |
| Hao, Y; Liu, P; Lv, Y; Ma, X; Xia, N | 1 |
1 review(s) available for nickel nitrilotriacetic acid and nitrilotriacetic acid
| Article | Year |
|---|---|
Engineering the lac permease for purification and crystallization.
Topics: Avidin; Crystallization; Cytochrome b Group; Drug Stability; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Membrane Transport Proteins; Monosaccharide Transport Proteins; Nitrilotriacetic Acid; Organometallic Compounds; Protein Engineering; Recombinant Fusion Proteins; Spectrophotometry; Symporters | 1996 |
1 trial(s) available for nickel nitrilotriacetic acid and nitrilotriacetic acid
| Article | Year |
|---|---|
Hemoglobin depletion from plasma: considerations for proteomic discovery in sickle cell disease and other hemolytic processes.
Topics: Adolescent; Anemia, Sickle Cell; Blood Proteins; Child; Child, Preschool; Chromatography, Affinity; Hemoglobins; Hemolysis; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Plasma; Proteomics | 2010 |
101 other study(ies) available for nickel nitrilotriacetic acid and nitrilotriacetic acid
| Article | Year |
|---|---|
Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni(2+)-NTA affinity chromatography.
Topics: Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Electron Transport Complex IV; Histidine; Molecular Sequence Data; Mutation; Nitrilotriacetic Acid; Oligodeoxyribonucleotides; Organometallic Compounds; Rhodobacter sphaeroides | 1995 |
Single-step purification of Proteus mirabilis urease accessory protein UreE, a protein with a naturally occurring histidine tail, by nickel chelate affinity chromatography.
Topics: Amino Acid Sequence; Bacterial Proteins; Carrier Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Genetic Complementation Test; Histidine; Molecular Sequence Data; Molecular Weight; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteus mirabilis; Recombinant Proteins; Sequence Analysis; Urease | 1994 |
Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization.
Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Arachidonate 12-Lipoxygenase; Arachidonic Acid; Baculoviridae; Cells, Cultured; Chromatography, Affinity; Cloning, Molecular; Histidine; Hydroxyeicosatetraenoic Acids; Kinetics; Leukocytes; Moths; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Precipitin Tests; Protein Engineering; Recombinant Fusion Proteins; Substrate Specificity; Swine | 1994 |
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Topics: Amino Acid Sequence; Bacterial Proteins; Chelating Agents; Chromatography, Affinity; Escherichia coli; Gene Expression; Genetic Vectors; Histidine; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Protein Denaturation; Recombinant Proteins; Recombination, Genetic | 1995 |
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Topics: Bacterial Proteins; Chromatography, Affinity; Escherichia coli; Genetic Testing; Genetic Vectors; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Recombinant Proteins; Resins, Plant; Solubility | 1996 |
A nickel chelate microtiter plate assay for six histidine-containing proteins.
Topics: Antibodies, Monoclonal; Chelating Agents; Enzyme-Linked Immunosorbent Assay; Histidine; Indicators and Reagents; Interleukin-8; Ligands; Lysine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Recombinant Proteins; Reproducibility of Results; Sensitivity and Specificity; Sequence Tagged Sites; Transferrin | 1996 |
Purification and refolding of recombinant human proMMP-7 (pro-matrilysin) expressed in Escherichia coli and its characterization.
Topics: Amino Acid Sequence; Base Sequence; Enzyme Activation; Enzyme Precursors; Escherichia coli; Gene Expression; Humans; Metalloendopeptidases; Molecular Sequence Data; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Phenylmercuric Acetate; Protein Folding; Recombinant Fusion Proteins; Resins, Plant; Substrate Specificity; Sulfhydryl Reagents; Transferrin | 1996 |
Development of magnetic beads for rapid and efficient metal-chelate affinity purifications.
Topics: Chromatography, Affinity; Histidine; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Polystyrenes; Resins, Synthetic; Tosyl Compounds | 1996 |
Gene disruptions indicate an essential function for the LmmCRK1 cdc2-related kinase of Leishmania mexicana.
Topics: Alleles; Animals; Blotting, Northern; Blotting, Southern; Blotting, Western; Caseins; Chromosome Mapping; DNA, Protozoan; Gene Expression Regulation; Genetic Complementation Test; Histidine; Leishmania mexicana; Molecular Sequence Data; Mutagenesis, Insertional; Nitrilotriacetic Acid; Organometallic Compounds; Plasmids; Ploidies; Protein Kinases; Protozoan Proteins; RNA, Protozoan; Sequence Deletion; Transfection; Trypanosoma brucei brucei | 1996 |
In vitro interactions of the aphid endosymbiotic SymL chaperonin with barley yellow dwarf virus.
Topics: Amino Acid Sequence; Animals; Aphids; Bacterial Proteins; Base Sequence; Centrifugation, Density Gradient; Chaperonin 60; Chaperonins; DNA; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Immunoblotting; Luteovirus; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sucrose; Symbiosis | 1997 |
Demonstration of dimer formation of the cytoplasmic domain of a transmembrane osmosensor protein, EnvZ, of Escherichia coli using Ni-histidine tag affinity chromatography.
Topics: Bacterial Outer Membrane Proteins; Chromatography, Affinity; Cytoplasm; Escherichia coli; Escherichia coli Proteins; Gene Expression; Histidine; Multienzyme Complexes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Fusion Proteins; Resins, Plant | 1997 |
The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA.
Topics: Affinity Labels; Animals; Chelating Agents; DNA, Single-Stranded; Histidine; Immunoglobulin Fab Fragments; Immunoglobulin Variable Region; In Vitro Techniques; Kinetics; Mice; Mutation; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Sepharose | 1997 |
Subunit interactions in the Escherichia coli protein translocase: SecE and SecG associate independently with SecY.
Topics: Bacterial Proteins; Chromatography, Affinity; Cloning, Molecular; Detergents; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Mutagenesis, Site-Directed; Nitrilotriacetic Acid; Octoxynol; Organometallic Compounds; Plasmids; Protein Binding; Protein Conformation; SEC Translocation Channels; Temperature | 1997 |
Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin.
Topics: Animals; ATP-Binding Cassette Transporters; Base Sequence; Carrier Proteins; Cathepsin D; DNA Primers; Enzyme Precursors; Escherichia coli; Escherichia coli Proteins; Factor Xa; Gene Expression; Genetic Vectors; Inclusion Bodies; Maltose-Binding Proteins; Monosaccharide Transport Proteins; Nitrilotriacetic Acid; Organometallic Compounds; Pepsinogens; Protein Conformation; Protein Folding; Recombinant Fusion Proteins; Solubility; Thioredoxins | 1998 |
A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants.
Topics: Animals; Antibodies; Antibodies, Antineutrophil Cytoplasmic; Antibody Specificity; Chromatography; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Escherichia coli; Histidine; Humans; Kidney Diseases; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Fragments; Peroxidase; Rabbits; Recombinant Proteins | 1998 |
Localization of a sigma70 binding site on the N terminus of the Escherichia coli RNA polymerase beta' subunit.
Topics: Amino Acid Sequence; Binding Sites; Conserved Sequence; DNA-Directed RNA Polymerases; Escherichia coli; Immunoblotting; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Mapping; Protein Binding; Sigma Factor | 1998 |
Strategies for protein-based nanofabrication: Ni2+-NTA as a chemical mask to control biologically imposed symmetry.
Topics: Biocompatible Materials; Biosensing Techniques; Chromatography, Affinity; Dimerization; Escherichia coli; Glutamate-Ammonia Ligase; Microscopy, Electron; Models, Molecular; Molecular Probes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Protein Conformation | 1998 |
Ni-NTA-gold clusters target His-tagged proteins.
Topics: Adenoviridae; Blotting, Western; Chromatography, Affinity; Forecasting; Gold; Histidine; Microscopy, Electron, Scanning Transmission; Molecular Probes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Receptors, Virus; Recombinant Proteins; Viral Structural Proteins | 1999 |
Purification of overexpressed hexahistidine-tagged BLM N431 as oligomeric complexes.
Topics: Adenosine Triphosphatases; Amino Acid Motifs; Animals; Bloom Syndrome; Chelating Agents; Chromatography, Affinity; Chromatography, Gel; DNA Helicases; Edetic Acid; Escherichia coli; Gene Expression; Histidine; Humans; Molecular Probes; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; RecQ Helicases; Solubility | 1999 |
Tandem affinity tags for the purification of bivalent anti-DNA single-chain Fv expressed in Escherichia coli.
Topics: Affinity Labels; Animals; Antibodies, Antinuclear; Antibody Specificity; Antigen-Antibody Reactions; Autoantibodies; Base Sequence; Chromatography, Affinity; Dimerization; DNA; DNA-Binding Proteins; Durapatite; Escherichia coli; Gene Expression; Humans; Immunoglobulin G; Immunoglobulin Variable Region; Leucine Zippers; Mice; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Periplasm; Protein Engineering; Recombinant Proteins; Sepharose | 1999 |
Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis.
Topics: Chemotactic Factors; Computer Systems; Flow Cytometry; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Gene Expression; GTP-Binding Proteins; Histidine; Humans; Microspheres; N-Formylmethionine Leucyl-Phenylalanine; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Receptors, Cell Surface; Receptors, Formyl Peptide; Receptors, Immunologic; Receptors, Peptide; Signal Transduction; Silicon Dioxide; Solubility; Spectrometry, Fluorescence; Transfection; U937 Cells | 2000 |
Self-assembling photosynthetic reaction centers on electrodes for current generation.
Topics: Biosensing Techniques; Electrochemistry; Electrodes; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Photosynthetic Reaction Center Complex Proteins; Rhodobacter sphaeroides; Surface Plasmon Resonance | 2000 |
Histidine-tagged ubiquitin substitutes for wild-type ubiquitin in Saccharomyces cerevisiae and facilitates isolation and identification of in vivo substrates of the ubiquitin pathway.
Topics: Arabidopsis; Canavanine; Chromatography, Ion Exchange; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Histidine; Immunoblotting; Mutagenesis, Site-Directed; Nitrilotriacetic Acid; Nitrogen; Organometallic Compounds; Plants, Genetically Modified; Plasmids; Protein Binding; RNA Polymerase II; Saccharomyces cerevisiae; Transfection; Ubiquitins; Yeasts | 2000 |
Interaction of sigma 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance.
Topics: Bacterial Proteins; Binding, Competitive; DNA-Directed RNA Polymerases; Enzyme Stability; Escherichia coli; Half-Life; Histidine; Holoenzymes; Kinetics; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Sigma Factor; Surface Plasmon Resonance | 2000 |
Purification of proteins using polyhistidine affinity tags.
Topics: Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Histidine; Indicators and Reagents; Membrane Proteins; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Recombinant Proteins | 2000 |
Receptor-dependent formation of COPI-coated vesicles from chemically defined donor liposomes.
Topics: ADP-Ribosylation Factor 1; Animals; Centrifugation, Density Gradient; Chromatography, Affinity; Coatomer Protein; COP-Coated Vesicles; Electrophoresis, Polyacrylamide Gel; GTPase-Activating Proteins; Humans; Lipoproteins; Liposomes; Liver; Membrane Proteins; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Fragments; Phosphatidylethanolamines; Proteolipids; Rabbits; Recombinant Proteins | 2001 |
Purification of functional Sec13p-Sec31p complex, a subunit of COPII coat.
Topics: Carrier Proteins; COP-Coated Vesicles; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Fungal Proteins; Macromolecular Substances; Mating Factor; Membrane Proteins; Monomeric GTP-Binding Proteins; Nitrilotriacetic Acid; Nuclear Pore Complex Proteins; Organometallic Compounds; Peptides; Phosphoproteins; Protein Subunits; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Vesicular Transport Proteins | 2001 |
Phage display of adenovirus type 5 fiber knob as a tool for specific ligand selection and validation.
Topics: Adenoviruses, Human; Capsid; Capsid Proteins; Cloning, Molecular; Histidine; Humans; Ligands; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library | 2001 |
Potential contact sites between the protein and RNA subunit in the Bacillus subtilis RNase P holoenzyme.
Topics: Bacillus subtilis; Bacterial Proteins; Base Sequence; Binding Sites; Catalytic Domain; Edetic Acid; Endoribonucleases; Ferrous Compounds; Holoenzymes; Iodine; Models, Molecular; Molecular Sequence Data; Nitrilotriacetic Acid; Nucleic Acid Conformation; Organometallic Compounds; Protein Binding; Protein Structure, Tertiary; Protein Subunits; Ribonuclease P; Ribonucleoproteins; RNA-Binding Proteins; RNA, Bacterial; RNA, Catalytic | 2002 |
Development and characterization of Ni-NTA-bearing microspheres.
Topics: Binding Sites; Chelating Agents; Flow Cytometry; Histidine; Indicators and Reagents; Microspheres; Nitrilotriacetic Acid; Organometallic Compounds; Surface Properties | 2002 |
Oligomerization characteristics of cysteine string protein.
Topics: Animals; Biopolymers; Electrophoresis, Polyacrylamide Gel; Hippocampus; HSP40 Heat-Shock Proteins; Membrane Proteins; Nerve Tissue Proteins; Neurons; Nitrilotriacetic Acid; Organometallic Compounds; Rats; Recombinant Fusion Proteins; Synapses | 2003 |
A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process.
Topics: CD11 Antigens; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cloning, Molecular; Escherichia coli; Flavodoxin; Gene Expression; Green Fluorescent Proteins; Humans; Hydrogen-Ion Concentration; Luminescent Proteins; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Fusion Proteins; Rubredoxins; Temperature | 2003 |
High affinity binding of serum histidine-rich glycoprotein to nickel-nitrilotriacetic acid: the application to microquantification.
Topics: Animals; Antibodies; Antibody Specificity; Chromatography, Affinity; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Immunochemistry; Indicators and Reagents; Microchemistry; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Proteins; Rabbits | 2003 |
Sequential and specific exchange of multiple coiled-coil components.
Topics: Alanine; Amino Acid Sequence; Cross-Linking Reagents; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Phenylalanine; Protein Structure, Quaternary | 2003 |
Reversible immobilization of engineered molecules by Ni-NTA chelators.
Topics: Adsorption; Biosensing Techniques; Chelating Agents; Coated Materials, Biocompatible; Electrochemistry; Electrodes; Light; Nitrilotriacetic Acid; Organometallic Compounds; Photosystem II Protein Complex; Protein Engineering; Recombinant Proteins | 2004 |
Orientated binding of photosynthetic reaction centers on gold using Ni-NTA self-assembled monolayers.
Topics: Adsorption; Atrazine; Biosensing Techniques; Coated Materials, Biocompatible; Crystallization; Electrochemistry; Equipment Design; Equipment Failure Analysis; Gold; Herbicides; Light; Macromolecular Substances; Materials Testing; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Photosynthetic Reaction Center Complex Proteins; Protein Binding; Protein Conformation | 2004 |
Purification of Na+,K+-ATPase expressed in Pichia pastoris reveals an essential role of phospholipid-protein interactions.
Topics: Adenosine Triphosphatases; Animals; Biochemistry; Blotting, Western; Cell Membrane; Chromatography; Chromatography, High Pressure Liquid; Culture Media; Electrophoresis, Polyacrylamide Gel; Genetic Vectors; Glucosides; Glycosylation; Ions; Kidney; Mass Spectrometry; Nitrilotriacetic Acid; Organometallic Compounds; Phosphatidylcholines; Phosphatidylserines; Phospholipids; Pichia; Plasmids; Potassium; Protein Binding; Protein Conformation; Protein Denaturation; Recombinant Proteins; Sodium-Potassium-Exchanging ATPase; Swine; Temperature; Time Factors | 2005 |
Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush.
Topics: Chelating Agents; Histidine; Nitrilotriacetic Acid; Organometallic Compounds; Polyethylene Glycols; Proteins; Sensitivity and Specificity | 2005 |
Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes.
Topics: Drug Delivery Systems; Humans; Immunoglobulin Fragments; Immunoglobulin Variable Region; In Vitro Techniques; Liposomes; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Protein Binding; Recombinant Proteins; Triglycerides | 2005 |
One-step fractionation of complex proteomes enables detection of low abundant analytes using antibody-based microarrays.
Topics: Chemical Fractionation; Cytokines; Glass; Humans; Immunoglobulin Variable Region; Molecular Weight; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Proteome; Proteomics | 2006 |
Real-time analysis of enzymatic surface-initiated polymerization using surface plasmon resonance (SPR).
Topics: Acyltransferases; Cupriavidus necator; Enzymes, Immobilized; Gold; Microscopy, Atomic Force; Nitrilotriacetic Acid; Organometallic Compounds; Polyesters; Recombinant Proteins; Silicon; Surface Plasmon Resonance | 2006 |
Purification of His-tagged hybrid phage antibody.
Topics: Animals; Antibodies, Monoclonal; Bacteriophages; Chromatography, Affinity; Genes, bcl-1; Histidine; Immunoglobulin Variable Region; Mice; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library | 2006 |
An evaluation of in vitro protein-protein interaction techniques: assessing contaminating background proteins.
Topics: Blotting, Far-Western; Chromatography, Affinity; Drug Contamination; Histidine; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Protein Interaction Mapping; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2006 |
Conformational changes in sarcoplasmic reticulum Ca(2+)-ATPase mutants: effect of mutations either at Ca(2+)-binding site II or at tryptophan 552 in the cytosolic domain.
Topics: Adenosine Triphosphate; Binding Sites; Calcium; Calcium-Transporting ATPases; Cations, Divalent; Chromatography, Affinity; Cytoplasm; Gene Expression; Glutamic Acid; Magnesium; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Phosphorus; Phosphorylation; Protein Conformation; Saccharomyces cerevisiae; Sarcoplasmic Reticulum; Tryptophan | 2006 |
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
Topics: Binding Sites; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Green Fluorescent Proteins; Histidine; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Array Analysis; Sensitivity and Specificity; Sepharose; Surface Properties; Time Factors | 2006 |
Synthesis of degenerated libraries of the ras-binding domain of raf and rapid selection of fast-folding and stable clones with the dihydrofolate reductase protein fragment complementation assay.
Topics: Chromatography, Affinity; Gene Library; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library; Protein Binding; Protein Engineering; Protein Folding; Protein Structure, Tertiary; Proto-Oncogene Proteins c-raf; ras Proteins; Tetrahydrofolate Dehydrogenase | 2007 |
In vitro characterization of binding and stability of single-chain Fv Ni-NTA-liposomes.
Topics: Antigens, CD; Blood Proteins; Cell Line, Tumor; Drug Delivery Systems; Drug Stability; Endoglin; Humans; Immunoglobulin Fragments; In Vitro Techniques; Liposomes; Nitrilotriacetic Acid; Organometallic Compounds; Phosphatidylethanolamines; Polyethylene Glycols; Protein Binding; Receptors, Cell Surface; Umbilical Veins | 2006 |
Design and preparation of beta-sheet forming repetitive and block-copolymerized polypeptides.
Topics: Amino Acid Sequence; Chromatography; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Library; Molecular Sequence Data; Molecular Weight; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Library; Peptides; Protein Structure, Secondary; Repetitive Sequences, Amino Acid | 2007 |
Variable stability heterodimeric coiled-coils from manipulation of electrostatic interface residue chain length.
Topics: Amino Acid Sequence; beta-Alanine; Dimerization; Glutamic Acid; Lysine; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Protein Structure, Secondary; Static Electricity | 2007 |
Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling.
Topics: Absorptiometry, Photon; Estrogen Receptor alpha; Fluorescent Dyes; Glass; Histidine; Ligands; Models, Molecular; Molecular Structure; Nanoparticles; Nitrilotriacetic Acid; Nuclear Magnetic Resonance, Biomolecular; Organometallic Compounds; Particle Size; Photochemistry; Protein Array Analysis; Silicon Dioxide; Solutions; Time Factors | 2007 |
Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size.
Topics: Adenoviridae; Chlorides; Glutathione; Gold; Gold Compounds; Histidine; Metal Nanoparticles; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Polymers; Protein Conformation; Viral Proteins | 2008 |
[Construction expression and biologic activity of recombinant human sCR1 eucaryotic cell].
Topics: Base Sequence; Calcium-Binding Proteins; Chromatography, Affinity; Cloning, Molecular; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Eukaryotic Cells; Gene Expression; Genetic Vectors; Humans; Membrane Proteins; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Pichia; Receptors, Complement 3b; Recombinant Fusion Proteins; Reverse Transcriptase Polymerase Chain Reaction | 2008 |
Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies.
Topics: 4-Chloro-7-nitrobenzofurazan; Calcium; Carbocyanines; Chromatography, Affinity; Dithionite; Escherichia coli; Gene Expression; Humans; Inclusion Bodies; Nitrilotriacetic Acid; Organometallic Compounds; Phosphatidylcholines; Phosphatidylethanolamines; Phospholipid Transfer Proteins; Protein Folding; Proteolipids; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Urea | 2008 |
Cryo-electron microscopy structure of a yeast mitochondrial preprotein translocase.
Topics: Cryoelectron Microscopy; Membrane Transport Proteins; Mitochondria; Mitochondrial Membrane Transport Proteins; Models, Molecular; Nitrilotriacetic Acid; Organometallic Compounds; Protein Subunits; Receptors, Cytoplasmic and Nuclear; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 2008 |
Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins.
Topics: Anti-Infective Agents; Antifungal Agents; Blotting, Western; Chromatography, Affinity; Cloning, Molecular; Defensins; Escherichia coli; Gene Expression; Genes, Plant; Inclusion Bodies; Nitrilotriacetic Acid; Organometallic Compounds; Plant Proteins; Protein Renaturation; Pseudomonas syringae; Recombinant Proteins; Solanum tuberosum | 2009 |
Immobilization of bacteriophage Qbeta on metal-derivatized surfaces via polyvalent display of hexahistidine tags.
Topics: Allolevivirus; Histidine; Metals; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Recombinant Fusion Proteins; Surface Plasmon Resonance | 2008 |
Analysis of nickel-binding peptides in a human hepidermoid cancer cell line by Ni-NTA affinity chromatography and mass spectrometry.
Topics: Actins; Amino Acid Sequence; Carcinoma, Squamous Cell; Cell Line, Tumor; Chromatography, Affinity; Humans; Molecular Sequence Data; Nickel; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Tandem Mass Spectrometry; Trypsin | 2008 |
A new quinoline sensitizer-centered lanthanide chelate and its use for protein labling on Ni-NTA beads for TR LRET assays.
Topics: Chelating Agents; Energy Transfer; Fluorescence Resonance Energy Transfer; Lanthanoid Series Elements; Microspheres; Models, Chemical; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Quinolines; Staining and Labeling; Time Factors | 2009 |
[Activity and quality comparison of the engineered protein Staphylococcus aureus alpha-hemolysin purified with gel filtration chromatography and Ni-NTA].
Topics: Chromatography, Gel; Escherichia coli; Hemolysin Proteins; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Proteins; Staphylococcus aureus | 2009 |
Sequential processing from cell lysis to protein assay on a chip enabling the optimization of an F(1)-ATPase single molecule assay condition.
Topics: Bacillus; Biotin; Cell Extracts; Diffusion; Electrodes; Enzyme Assays; Escherichia coli; Histidine; Intracellular Space; Microspheres; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Proton-Translocating ATPases; Streptavidin; Time Factors | 2009 |
Compact and versatile nickel-nitrilotriacetate-modified quantum dots for protein imaging and Förster resonance energy transfer based assay.
Topics: Cadmium Compounds; Cell Line; Fluorescence Resonance Energy Transfer; Histidine; Humans; Ligands; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Quantum Dots; Recombinant Proteins; Selenium Compounds; Solubility; Sulfides; Water; Zinc Compounds | 2010 |
Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.
Topics: Alkyl and Aryl Transferases; Carbocyanines; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Histidine; N-Ethylmaleimide-Sensitive Proteins; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Interaction Domains and Motifs; Spectrometry, Fluorescence; Ultracentrifugation | 2010 |
Separation of alpha- from beta-arylalanines by nickel nitrilotriacetate chromatography.
Topics: Alanine; Chelating Agents; Chromatography, Liquid; Gas Chromatography-Mass Spectrometry; Nitrilotriacetic Acid; Organometallic Compounds; Spectrophotometry, Ultraviolet | 2010 |
Purification and refolding of Escherichia coli-expressed recombinant human interleukin-2.
Topics: Chromatography, Affinity; Escherichia coli; Humans; Inclusion Bodies; Interleukin-2; Nitrilotriacetic Acid; Organometallic Compounds; Protein Folding; Recombinant Proteins; Solubility | 2010 |
Surface functionalization of polyketal microparticles with nitrilotriacetic acid-nickel complexes for efficient protein capture and delivery.
Topics: Animals; Cell Line; Endothelial Cells; Green Fluorescent Proteins; Humans; Macrophages; Mice; Microspheres; Mitochondria; Muscle Cells; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Surface Properties; Vascular Endothelial Growth Factor A | 2010 |
Influence of multivalent nitrilotriacetic acid lipid-ligand affinity on the circulation half-life in mice of a liposome-attached His6-protein.
Topics: Animals; Half-Life; Histidine; Ligands; Lipids; Liposomes; Mice; Mice, Inbred BALB C; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Proteins | 2010 |
Cross-linking of trans reentrant loops in the Na(+)-citrate transporter CitS of Klebsiella pneumoniae.
Topics: Amino Acid Motifs; Blood Proteins; Carrier Proteins; Citric Acid; Cysteine; Intracellular Signaling Peptides and Proteins; Klebsiella pneumoniae; Nitrilotriacetic Acid; Organometallic Compounds; Protein Serine-Threonine Kinases; Protein Structure, Tertiary | 2010 |
Activation of human VPS4A by ESCRT-III proteins reveals ability of substrates to relieve enzyme autoinhibition.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Motifs; Amino Acid Sequence; ATPases Associated with Diverse Cellular Activities; Binding Sites; Endosomal Sorting Complexes Required for Transport; Enzyme Activation; Histidine; Humans; Hydrolysis; Liposomes; Molecular Sequence Data; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Protein Multimerization; Substrate Specificity; Vacuolar Proton-Translocating ATPases | 2010 |
Localized cell uptake of His-tagged polyplexes immobilized on NTA self-assembled monolayers.
Topics: Animals; Edetic Acid; Flow Cytometry; Histidine; Imidazoles; Mice; NIH 3T3 Cells; Nitrilotriacetic Acid; Organometallic Compounds; Surface Plasmon Resonance | 2010 |
A filter microplate assay for quantitative analysis of DNA binding proteins using fluorescent DNA.
Topics: Binding, Competitive; Carbon Radioisotopes; DNA; Fluorescent Dyes; Homeodomain Proteins; Humans; Nanog Homeobox Protein; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Recombinant Proteins; SOXB1 Transcription Factors; Spectrometry, Fluorescence | 2011 |
Overexpression of (His)6-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography.
Topics: Arginase; Chromatography, Affinity; Cloning, Molecular; Histidine; Humans; Liver; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Recombinant Fusion Proteins; Saccharomyces cerevisiae | 2012 |
Formation of high-capacity protein-adsorbing membranes through simple adsorption of poly(acrylic acid)-containing films at low pH.
Topics: Acrylic Resins; Adsorption; Animals; Cattle; Humans; Hydrogen-Ion Concentration; Hydroxylation; Membranes, Artificial; Nitrilotriacetic Acid; Nylons; Organometallic Compounds; Permeability; Polyamines; Polyethyleneimine; Proteins | 2012 |
Unequivocal single-molecule force spectroscopy of intrinsically disordered proteins.
Topics: Cloning, Molecular; Glass; Humans; Microscopy, Atomic Force; Nanotechnology; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Termination Factors; Proteins; Saccharomyces cerevisiae Proteins | 2012 |
Detection of PCNA modifications in Saccharomyces cerevisiae.
Topics: Blotting, Western; Chromatography, Affinity; DNA Damage; Electrophoresis, Polyacrylamide Gel; Nitrilotriacetic Acid; Organometallic Compounds; Proliferating Cell Nuclear Antigen; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; SUMO-1 Protein; Ubiquitination | 2012 |
RNA transfer from poliovirus 135S particles across membranes is mediated by long umbilical connectors.
Topics: Electron Microscope Tomography; Liposomes; Models, Biological; Nitrilotriacetic Acid; Organometallic Compounds; Poliovirus; Receptors, Virus; RNA, Viral; Temperature; Virus Attachment; Virus Internalization | 2013 |
Ostreopexin: a hemopexin fold protein from the oyster mushroom, Pleurotus ostreatus.
Topics: Albumins; Chromatography, Liquid; Fabaceae; Fungal Proteins; Hemin; Hemopexin; Nitrilotriacetic Acid; Organometallic Compounds; Oryza; Plant Proteins; Pleurotus; Polyamines; Protein Conformation; Recombinant Proteins; Seeds; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry | 2013 |
Label-free fluorescent detection of thrombin activity based on a recombinant enhanced green fluorescence protein and nickel ions immobilized nitrilotriacetic acid-coated magnetic nanoparticles.
Topics: Antithrombins; Equipment Reuse; Green Fluorescent Proteins; Hirudins; Histidine; Humans; Immobilized Proteins; Limit of Detection; Magnetite Nanoparticles; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Proteolysis; Recombinant Fusion Proteins; Reproducibility of Results; Spectrometry, Fluorescence; Thrombin | 2013 |
Capturing enveloped viruses on affinity grids for downstream cryo-electron microscopy applications.
Topics: Cryoelectron Microscopy; Nitrilotriacetic Acid; Organometallic Compounds; Virion; Viruses | 2014 |
Thermodynamics of imidazole-ligand binding to Ni-nitrilotriacetate in solution and covalently attached to agarose beads: imidazole, his-6 (his-tag) peptide and a new bis-imidazolo-dithiane.
Topics: Calorimetry; Chromatography, Affinity; Histidine; Imidazoles; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Protein Binding; Thermodynamics | 2014 |
Identification of Small-Molecule Frequent Hitters from AlphaScreen High-Throughput Screens.
Topics: Automation; Biological Assay; Drug Discovery; Escherichia coli; Fluorescence Resonance Energy Transfer; High-Throughput Screening Assays; Kinetics; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; Protein Interaction Mapping; Recombinant Proteins; Small Molecule Libraries | 2014 |
Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs).
Topics: HEK293 Cells; HeLa Cells; Histidine; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Protein Binding; rac1 GTP-Binding Protein; Ubiquitination; X-Linked Inhibitor of Apoptosis Protein | 2014 |
Reconstituting functional microtubule-barrier interactions.
Topics: Animals; Biotinylation; Cattle; Dyneins; Fluorescence Recovery After Photobleaching; Glass; Gold; Immobilized Proteins; Microtechnology; Microtubules; Nitrilotriacetic Acid; Organometallic Compounds; Polymerization; Serum Albumin, Bovine; Sus scrofa | 2014 |
Localizing chemical groups while imaging single native proteins by high-resolution atomic force microscopy.
Topics: Binding Sites; Histidine; Inorganic Chemicals; Ligands; Microscopy, Atomic Force; Molecular Imaging; Nanotechnology; Nitrilotriacetic Acid; Organometallic Compounds; Proteins | 2014 |
Targeted in vivo photodynamic therapy with epidermal growth factor receptor-specific peptide linked nanoparticles.
Topics: Animals; Cell Line, Tumor; Cell Survival; Chitosan; Drug Carriers; Gold; Ligands; Magnetite Nanoparticles; Mice, Nude; Nitrilotriacetic Acid; Organometallic Compounds; Particle Size; Peptides; Photochemotherapy; Photosensitizing Agents; Porphyrins; Receptor, ErbB-2; Recombinant Fusion Proteins; Surface Properties; Thioctic Acid; Xenograft Model Antitumor Assays | 2014 |
Metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing α-L-rhamnosidase from Bifidobacterium dentium.
Topics: Base Sequence; Bifidobacterium; Cloning, Molecular; Cloning, Organism; Escherichia coli; Flavanones; Flavonoids; Ginsenosides; Glycoside Hydrolases; Humans; Intestines; Microbiota; Molecular Sequence Data; Nitrilotriacetic Acid; Organometallic Compounds; Quercetin; Rutin; Sequence Alignment; Sequence Analysis, DNA; Substrate Specificity | 2015 |
Ni(II)NTA AuNPs as a low-resource malarial diagnostic platform for the rapid colorimetric detection of Plasmodium falciparum Histidine-Rich Protein-2.
Topics: Antigens, Protozoan; Colorimetry; Gold; Humans; Malaria; Metal Nanoparticles; Nitrilotriacetic Acid; Organometallic Compounds; Plasmodium falciparum; Protozoan Proteins; Recombinant Proteins; Serum Albumin | 2015 |
A colorimetric-based amplification system for proteinases including MMP2 and ADAM8.
Topics: ADAM Proteins; Amino Acid Sequence; Benzidines; Colorimetry; Dipeptides; Enzyme Assays; Horseradish Peroxidase; Humans; Hydrogen Peroxide; Kinetics; Magnets; Matrix Metalloproteinase 2; Matrix Metalloproteinase Inhibitors; Membrane Proteins; Microspheres; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Substrate Specificity | 2015 |
Controlled immobilization of His-tagged proteins for protein-ligand interaction experiments using Ni²⁺-NTA layer on glass surfaces.
Topics: Biosensing Techniques; Glass; Histidine; Ligands; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; S100 Proteins | 2015 |
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization.
Topics: Alkaloids; Cell Line; Chromatography, Affinity; Crystallization; Crystallography, X-Ray; Glycoproteins; HEK293 Cells; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Polyethyleneimine; Recombinant Proteins; Transfection | 2015 |
Mode of Ezrin-Membrane Interaction as a Function of PIP2 Binding and Pseudophosphorylation.
Topics: Cytoskeletal Proteins; Escherichia coli; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; Lipid Bilayers; Microscopy, Atomic Force; Microscopy, Fluorescence; Models, Molecular; Mutation; Nitrilotriacetic Acid; Organometallic Compounds; Phosphatidylinositol 4,5-Diphosphate; Phosphorylation; Protein Binding; Protein Conformation; Recombinant Proteins; Spectrum Analysis | 2016 |
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases | 2018 |
Simple and efficient knockdown of His-tagged proteins by ternary molecules consisting of a His-tag ligand, a ubiquitin ligase ligand, and a cell-penetrating peptide.
Topics: Cell Line; Cell-Penetrating Peptides; Dose-Response Relationship, Drug; Histidine; Humans; Leucine; Ligands; Molecular Structure; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Receptors, Retinoic Acid; Structure-Activity Relationship; Ubiquitin-Protein Ligases | 2017 |
Orthogonal supramolecular protein assembly on patterned bifunctional surfaces.
Topics: Adamantane; beta-Cyclodextrins; Green Fluorescent Proteins; Histidine; Nickel; Nitrilotriacetic Acid; Oligopeptides; Organometallic Compounds; Surface Properties | 2018 |
Affinity Purification of Methyllysine Proteome by Site-Specific Covalent Conjugation.
Topics: Chromobox Protein Homolog 5; Humans; Lysine; MCF-7 Cells; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Proteome; src Homology Domains | 2018 |
Modification of phage display technique for improved screening of high-affinity binding peptides.
Topics: Bacteriophages; Cell Surface Display Techniques; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Nitrilotriacetic Acid; Organometallic Compounds; Peptides; Protein Binding; Receptor, ErbB-2; Silicon Dioxide | 2019 |
Folding and Assembly of Vanilloid Receptor Secondary-Structure Peptide with Hexahistidine Linker at Nickel-Nitrilotriacetic Acid Monolayer for Capsaicin Recognition.
Topics: Amino Acid Sequence; Capsaicin; Circular Dichroism; Membranes, Artificial; Microscopy, Atomic Force; Nitrilotriacetic Acid; Organometallic Compounds; Peptide Fragments; Protein Binding; Protein Conformation, alpha-Helical; Protein Domains; Protein Engineering; Quartz Crystal Microbalance Techniques; TRPV Cation Channels | 2019 |
Protein Microarray Copying: Easy on-Demand Protein Microarray Generation Compatible with Fluorescence and Label-Free Real-Time Analysis.
Topics: Fluorescence; Nitrilotriacetic Acid; Organometallic Compounds; Protein Array Analysis; Proteins; Surface Properties | 2019 |
Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.
Topics: Cations, Divalent; Chelating Agents; Chromatography, Affinity; Electron Transport Complex III; Metals; Naphthalenesulfonates; Nitrilotriacetic Acid; Organometallic Compounds; Recombinant Proteins; Transition Elements | 2019 |
Ultrafast in-gel detection by fluorescent super-chelator probes with HisQuick-PAGE.
Topics: Animals; Chelating Agents; Electrophoresis, Polyacrylamide Gel; Escherichia; Fluorescent Dyes; HeLa Cells; Histidine; Humans; Nitrilotriacetic Acid; Organometallic Compounds; Proteins; Sf9 Cells; Workflow | 2020 |
Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein.
Topics: Aptamers, Nucleotide; Cadherins; Ligands; Nitrilotriacetic Acid; Organometallic Compounds; SELEX Aptamer Technique | 2020 |
Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.
Topics: Fluorescence; Histidine; Magnetite Nanoparticles; Nickel; Nitrilotriacetic Acid; Peptide Hydrolases; Peptides | 2023 |