neuropeptide-y and zaprinast

Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.

Topics: Animals; Blotting, Northern; Enzyme Inhibitors; Immunohistochemistry; Molsidomine; Nerve Growth Factors; Neuropeptide Y; Nitric Oxide; Nitric Oxide Synthase; Nitroprusside; PC12 Cells; Pheochromocytoma; Phosphodiesterase Inhibitors; Purinones; Radioimmunoassay; Rats; RNA; Sodium-Potassium-Exchanging ATPase