neuropeptide-y has been researched along with 2-(2-aminoethyl)pyridine* in 1 studies
1 other study(ies) available for neuropeptide-y and 2-(2-aminoethyl)pyridine
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Intra-articular injection of 2-pyridylethylamine produces spinal NPY-mediated antinociception in the formalin-induced rat knee-joint pain model.
Low doses of histamine or H1R agonist 2-pyridylethylamine (2-PEA) into the knee-joint were found to decrease formalin-induced articular nociception in rats. In this study, we evaluated the participation of spinal NPY in the antinociceptive effect produced by 2-PEA. Injection of formalin (1.5%) into one of the knee-joints causes the limping of the respective limb due to nociception, which was registered each 5 min over 60 min. Neuropeptide Y1 receptor (Y1R) content in the spinal cord was evaluated by western-blotting. Intrathecal (i.t.) injection of Y1R agonist Leu31, Pro34-NPY (0.7-7 µmol) decreased nociception, while injection of the antagonist BIBO 3304 (4 μmol), increased nociception. Antinociception produced by 2-PEA was reversed by a sub-effective i.t. dose of the Y1R antagonist. Similarly, this antinociceptive effect was prevented by i.t. pretreatment with the neurotoxin NPY-saporin (750 ng), which also reduced immunoblotting for Y1R in spinal cord homogenates. These data support the idea that antinociception induced by H1R agonists in the knee-joint of rats may be mediated by the spinal release of NPY, and this peptide seems to be acting via Y1R. Topics: Analgesics; Animals; Arthralgia; Hindlimb; Injections, Intra-Articular; Injections, Spinal; Knee Joint; Male; Neuropeptide Y; Nociception; Pain; Pain Measurement; Pyridines; Rats; Rats, Wistar; Receptors, Histamine; Receptors, Neuropeptide Y; Spinal Cord; Spine | 2020 |