neoxanthin and alpha-carotene

The chemical and biological properties of carotenoids in the freshwater alga Oedogonium intermedium were investigated in this study. Carotenoids were extracted from the alga by dichloromethane and purified by saponification. The carotenoid content was determined both spectrometrically and by HPLC, the carotenoids identified by HPLC-PDA-APCI-IT-TOF-MS and the extracts analysed for several health-related bioactivities. The crude and saponified extracts contained 3,411.2±20.7 and 2,929.6±5.9µg carotenoids/g dry algal biomass, respectively. Seven major carotenoids were identified, namely neoxanthin, 9'-cis-neoxanthin, loroxanthin, violaxanthin, lutein, α-carotene and β-carotene, which were present in similar amounts in the alga. Both the crude and saponified carotenoid extracts exhibited significant antioxidant activities as well as potent inhibitory effects against several metabolically important enzymes including α-amylase, α-glucosidase, pancreatic lipase and hyaluronidase, but they were poor inhibitors of angiotensin converting enzyme (ACE). Oedogonium could be an important new source of carotenoids, specifically loroxanthin, which is lacking in terrestrial plants.

Topics: alpha-Amylases; Animals; Antioxidants; beta Carotene; Carotenoids; Chlorophyta; Chromatography, High Pressure Liquid; Drug Evaluation, Preclinical; Enzyme Inhibitors; Fresh Water; Glycoside Hydrolase Inhibitors; Hyaluronoglucosaminidase; Lipase; Lutein; Xanthophylls

The objective of this study was to evaluate the effects of irradiance levels and spectra produced by solid-state light-emitting diodes (LEDs) on carotenoid content and composition changes in Brassicaceae microgreens. A system of five high-power, solid-state lighting modules with standard 447-, 638-, 665-, and 731-nm LEDs was used in the experiments. Two experiments were performed: (1) evaluation of LED irradiance levels of 545, 440, 330, 220, and 110 μmol m(-2) s(-1) photosynthetically active flux density (PPFD) and (2) evaluation of the effects of 520-, 595-, and 622-nm LEDs supplemental to the standard set of LEDs. Concentrations of various carotenoids in red pak choi and tatsoi were higher under illumination of 330-440 μmol m(-2) s(-1) and at 110-220 μmol m(-2) s(-1) in mustard. All supplemental wavelengths increased total carotenoid content in mustard but decreased it in red pak choi. Carotenoid content increased in tatsoi under supplemental yellow light.

Topics: beta Carotene; Brassicaceae; Carotenoids; Light; Lighting; Lutein; Photosynthesis; Plant Leaves; Xanthophylls

To cope with environmental stress, plants are equipped with antioxidative (e.g., ascorbate, glutathione and alpha-tocopherol) and photoprotective (e.g., xanthophyll cycle pigments) defense systems. We investigated the defense capacities of three tree age classes (mature, sapling and seedling) of Norway spruce (Picea abies (L.) Karst.) at a field site near the timberline. Biochemical data were expressed on both a needle dry mass and a surface area basis. Compared with current-year needles, previous-year needles contained higher mass- and area-based concentrations of chlorophylls and alpha-tocopherol, and a larger xanthophyll cycle pool that was in a more epoxidized state. Total glutathione concentration was lower, the glutathione pool was more reduced and the ascorbate pool was more oxidized in previous-year needles than in current-year needles. Needle concentrations of glutathione and alpha-tocopherol increased and chlorophyll concentration decreased with increasing tree age when expressed on a surface area basis. On a dry mass basis, these trends were reversed or nonexistent. The ascorbate pool was more reduced and the glutathione pool was more oxidized in needles of mature trees than in needles of saplings and seedlings. The proportion of protective xanthophyll cycle pigments decreased and the de-epoxidation state increased with increasing tree age. We conclude that tree age and the basis of expression of antioxidant concentration--surface area or dry mass--are important in scaling from seedlings to large trees.

Topics: alpha-Tocopherol; Antioxidants; Ascorbic Acid; beta Carotene; Carotenoids; Chlorophyll; Glutathione; Picea; Pigments, Biological; Plant Leaves; Trees; Xanthophylls