negletein and astilbin

Six phenolic constituents, 2-methoxybenzyl benzoate (1), negletein (2), 2',3'-dihydroxy-4',6'-dimethoxydihydrochalcone (3), 5,6-dihydroxy-7-methoxy-dihydroflavone (4), astilbin (5), and quercitrin (6) were isolated from the methanol extract of the dried leaves of Desmos chinensis. Their structures were elucidated from spectral and chemical data. Of these constituents, compounds 2 (IC50: 3.89 +/- 0.39 microM) and 3 (IC50: 9.77 +/- 0.26 microM) exhibited potent inhibitory activity against nuclear factor of activated T cells (NFAT) transcription factor, and compound 1 (IC50: 28.4 +/- 2.62 microM) exhibited moderate inhibitory activity.

Topics: Annonaceae; Benzoates; Benzyl Compounds; Cell Survival; Chalcones; Flavanones; Flavones; Flavonols; Humans; Inhibitory Concentration 50; Jurkat Cells; Methanol; NFATC Transcription Factors; Phenols; Plant Extracts; Plant Leaves; Plants, Medicinal; Quercetin