n-methyl-n-(tert-butyldimethylsilyl)trifluoroacetamide has been researched along with thymine-glycol* in 1 studies
1 other study(ies) available for n-methyl-n-(tert-butyldimethylsilyl)trifluoroacetamide and thymine-glycol
| Article | Year |
|---|---|
Determination of cis-thymine glycol in DNA by gas chromatography-mass spectrometry with selected ion recording and multiple reaction monitoring.
A novel method for the determination of cis-thymine glycol in DNA has been developed, using gas chromatography-mass spectrometry with selected ion recording or multiple reaction monitoring. The procedure involves acidic hydrolysis of DNA in the presence of the internal standard cis-[2H3]thymine glycol, followed by derivatisation with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide. The method was validated on DNA that had been oxidatively modified in vitro by radiation treatment, and was then applied to determine cis-thymine glycol in human placental DNA. Background levels of 5.45+/-2.98 ng cis-thymine glycol/mg DNA were observed in the human samples. Topics: Acetamides; Animals; Antioxidants; Calibration; Cattle; DNA; Female; Fluoroacetates; Gas Chromatography-Mass Spectrometry; Humans; Hydrolysis; Organosilicon Compounds; Osmolar Concentration; Placenta; Pregnancy; Reference Standards; Stereoisomerism; Thymine; Thymus Gland; Trifluoroacetic Acid; Tritium | 1997 |