n-formyltryptophan and trimethylsilyl-bromide

n-formyltryptophan has been researched along with trimethylsilyl-bromide* in 1 studies

Other Studies

1 other study(ies) available for n-formyltryptophan and trimethylsilyl-bromide

Article	Year
Two-step hard acid deprotection/cleavage procedure for solid phase peptide synthesis. International journal of peptide and protein research, 1991, Volume: 37, Issue:2 A new two-step deprotection/cleavage procedure for t-butoxycarbonyl (Boc) based solid phase peptide synthesis is reported. First the protective groups are removed from 4-(oxymethyl)-phenylacetamidomethyl (PAM) resin attached peptide with the weak hard acid, trimethylsilyl bromide-thioanisole/trifluoroacetic acid (TFA). In the second step, the peptide is cleaved from the resin with a stronger hard acid such as trimethylsilyl trifluoromethanesulfonate in TFA or with HF. The method is also shown to deformylate Nin-formyltryptophan moiety efficiently. The usefulness of this procedure for practical solid phase peptide synthesis is demonstrated by comparison with other deprotection methods in the synthesis of urotensin II and human endothelin. Topics: Acetamides; Amino Acid Sequence; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Endothelins; Formic Acid Esters; Humans; Indicators and Reagents; Molecular Sequence Data; Peptides; Phenylacetates; Resins, Plant; Trifluoroacetic Acid; Trimethylsilyl Compounds; Tryptophan; Urotensins	1991

Article

Year

Two-step hard acid deprotection/cleavage procedure for solid phase peptide synthesis.

International journal of peptide and protein research, 1991, Volume: 37, Issue:2

A new two-step deprotection/cleavage procedure for t-butoxycarbonyl (Boc) based solid phase peptide synthesis is reported. First the protective groups are removed from 4-(oxymethyl)-phenylacetamidomethyl (PAM) resin attached peptide with the weak hard acid, trimethylsilyl bromide-thioanisole/trifluoroacetic acid (TFA). In the second step, the peptide is cleaved from the resin with a stronger hard acid such as trimethylsilyl trifluoromethanesulfonate in TFA or with HF. The method is also shown to deformylate Nin-formyltryptophan moiety efficiently. The usefulness of this procedure for practical solid phase peptide synthesis is demonstrated by comparison with other deprotection methods in the synthesis of urotensin II and human endothelin.

Topics: Acetamides; Amino Acid Sequence; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Endothelins; Formic Acid Esters; Humans; Indicators and Reagents; Molecular Sequence Data; Peptides; Phenylacetates; Resins, Plant; Trifluoroacetic Acid; Trimethylsilyl Compounds; Tryptophan; Urotensins

1991