n-acetylgalactosamine-1-phosphate and thymidine-5--triphosphate

n-acetylgalactosamine-1-phosphate has been researched along with thymidine-5--triphosphate* in 1 studies

Other Studies

1 other study(ies) available for n-acetylgalactosamine-1-phosphate and thymidine-5--triphosphate

ArticleYear
Investigation of the nucleotide triphosphate substrate specificity of Homo sapiens UDP-N-acetylgalactosamine pyrophosphorylase (AGX1).
    Bioorganic & medicinal chemistry letters, 2012, Jun-15, Volume: 22, Issue:12

    Nucleotide sugars are essential glycosyl donors for Leloir-type glycosyltransferases. The UDP-N-acetylgalactosamine pyrophosphorylase (UDP-GalNAc PP; AGX1) from Homo sapiens catalyzes the synthesis of UDP-N-acetylgalactosamine from N-acetylgalactosamine 1-phosphate and UTP. In this Letter, we systematically studied nucleotide substrate specificity of AGX1 during its uridyltransfer reaction, and described the capability of AGX1 to catalyze dUTP and dTTP to their corresponding nucleotide sugars for the first time. Furthermore, using such a eukaryotic enzyme, we synthesized dUDP-GalNAc and dTDP-GalNAc in multiple mg scale in vitro efficiently and rapidly.

    Topics: Acetylgalactosamine; Biocatalysis; Escherichia coli; Galactosyltransferases; Humans; Kinetics; Recombinant Proteins; Substrate Specificity; Thymine Nucleotides; Uridine Diphosphate N-Acetylgalactosamine; Uridine Triphosphate

2012