n(7)-hydroxyethylguanine and 1-(2-chloroethyl)-1-nitrosourea

n(7)-hydroxyethylguanine has been researched along with 1-(2-chloroethyl)-1-nitrosourea* in 1 studies

Other Studies

1 other study(ies) available for n(7)-hydroxyethylguanine and 1-(2-chloroethyl)-1-nitrosourea

Article	Year
Detection of N7-(2-hydroxyethyl)guanine adducts in DNA and 9L cells treated with 1-(2-chloroethyl)-1-nitrosourea. Journal of chromatography. B, Biomedical sciences and applications, 1997, Jun-20, Volume: 694, Issue:1 A sensitive analytical method, HPLC-ED, was developed for the measurement of N7-(2-hydroxyethyl)guanine (N7-HOEtG). A detection limit of 3.2 N7-HOEtG/10(8) nucleotides was obtained with this method. Linear dose response curves for the formation of N7-HOEtG were obtained following treatment of either calf thymus DNA or 9L cells with 1-(2-chloroethyl)-1-nitrosourea (CNU). Using HPLC-ED a significant increase in the level of N7-HOEtG could be detected in 9L cells following treatment with 5 microM CNU. Our study suggests that with this analytical method the formation of N7-HOEtG in the white blood cells of patients treated with chloroethylnitrosoureas may be determined. Topics: Animals; Antineoplastic Agents; Cattle; Chromatography, High Pressure Liquid; DNA; DNA Adducts; Electrochemistry; Ethylnitrosourea; Guanine; L Cells; Mice; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet	1997

Article

Year

Detection of N7-(2-hydroxyethyl)guanine adducts in DNA and 9L cells treated with 1-(2-chloroethyl)-1-nitrosourea.

Journal of chromatography. B, Biomedical sciences and applications, 1997, Jun-20, Volume: 694, Issue:1

A sensitive analytical method, HPLC-ED, was developed for the measurement of N7-(2-hydroxyethyl)guanine (N7-HOEtG). A detection limit of 3.2 N7-HOEtG/10(8) nucleotides was obtained with this method. Linear dose response curves for the formation of N7-HOEtG were obtained following treatment of either calf thymus DNA or 9L cells with 1-(2-chloroethyl)-1-nitrosourea (CNU). Using HPLC-ED a significant increase in the level of N7-HOEtG could be detected in 9L cells following treatment with 5 microM CNU. Our study suggests that with this analytical method the formation of N7-HOEtG in the white blood cells of patients treated with chloroethylnitrosoureas may be determined.

Topics: Animals; Antineoplastic Agents; Cattle; Chromatography, High Pressure Liquid; DNA; DNA Adducts; Electrochemistry; Ethylnitrosourea; Guanine; L Cells; Mice; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet

1997