n(2)-n(2)-dimethylguanosine and 5-hydroxymethyl-2--deoxyuridine

Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa).. Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction.. Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients.. This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adenosine; Adult; Breast Neoplasms; Chromatography, Liquid; Deoxyguanosine; Female; Guanosine; Humans; Mastectomy; Middle Aged; Nucleosides; Oxidative Stress; Postoperative Period; Reactive Oxygen Species; Tandem Mass Spectrometry; Thymidine