myelin-oligodendrocyte-glycoprotein-(35-55) and tricine

myelin-oligodendrocyte-glycoprotein-(35-55) has been researched along with tricine* in 1 studies

Other Studies

1 other study(ies) available for myelin-oligodendrocyte-glycoprotein-(35-55) and tricine

Article	Year
Conjugation of a peptide to mannan and its confirmation by tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analytical biochemistry, 2015, Sep-15, Volume: 485 The conjugation of polysaccharides to peptides is essential for antigen delivery and vaccine development. Herein, we show that tricine SDS-PAGE in combination with Coomassie Blue staining was adequate to determine the conjugation efficacy of a peptide (epitope 35-55 of myelin oligodendrocyte glycoprotein) to mannan. In addition, tricine SDS-PAGE and periodic acid-Schiff stains were able to monitor the redox state of mannan. Using the described protocol, more than 99.9% of a peptide containing five lysines at its N-terminus was confirmed conjugated to mannan. Topics: Electrophoresis, Polyacrylamide Gel; Glycine; Mannans; Myelin-Oligodendrocyte Glycoprotein; Peptide Fragments	2015

Article

Year

Conjugation of a peptide to mannan and its confirmation by tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Analytical biochemistry, 2015, Sep-15, Volume: 485

The conjugation of polysaccharides to peptides is essential for antigen delivery and vaccine development. Herein, we show that tricine SDS-PAGE in combination with Coomassie Blue staining was adequate to determine the conjugation efficacy of a peptide (epitope 35-55 of myelin oligodendrocyte glycoprotein) to mannan. In addition, tricine SDS-PAGE and periodic acid-Schiff stains were able to monitor the redox state of mannan. Using the described protocol, more than 99.9% of a peptide containing five lysines at its N-terminus was confirmed conjugated to mannan.

Topics: Electrophoresis, Polyacrylamide Gel; Glycine; Mannans; Myelin-Oligodendrocyte Glycoprotein; Peptide Fragments

2015