myelin-basic-protein and pixantrone

Pixantrone is an immunesuppressor similar to mitoxantrone but with lower cardiotoxicity. We evaluated the effect of pixantrone on B cells and lymphomononuclear cells in the course of acute EAE. Pixantrone reduced the number of B cells and suppressed myelin basic protein (MBP) specific IgG production. In vitro, pixantrone induced apoptosis of rat B lymphocytes in a way similar to mitoxantrone. In addition, pixantrone inhibited antigen specific and mitogen induced lymphomononuclear cell proliferation, as well as IFN-gamma production, during EAE. These findings suggest a similar mechanism of action for pixantrone and mitoxantrone on the effector function of lymphomonocyte B and T cells.

Topics: Analysis of Variance; Animals; Antibodies; Antigens, CD; Apoptosis; B-Lymphocytes; Cell Proliferation; Cytokines; Cytokinesis; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Immunosuppressive Agents; Isoquinolines; Myelin Basic Protein; Rats; Rats, Inbred Lew; T-Lymphocytes; Time Factors