myelin-basic-protein and 2-butanol

A rapid procedure for purification of myelin basic protein has been developed. White matter is delipidated with 2-butanol, and the residue is extracted at pH 7.5 and 8.5. Myelin basic protein is solubilized by extraction in acetate buffer, pH 4.5. The entire procedure requires less than 4 h, and gives homogeneous protein essentially free of protease activity. This procedure can be scaled down to process milligram amounts of white matter; thus it can be very useful for purification of myelin basic protein from very limited amounts of human white matter obtained during surgery.

Topics: Acetates; Acetic Acid; Animals; Brain Chemistry; Buffers; Butanols; Cattle; Chromatography; Electrophoresis, Polyacrylamide Gel; Humans; Myelin Basic Protein; Peptide Hydrolases