muramidase and pyrazolone

Chemoselective, biocompatible ligation reactions are the key components for efficient and modular access to biomolecular scaffolds. Tetrazine ligation leads to the formation of a mixture of isomers, which makes reaction monitoring, purification and characterization of conjugates difficult. We report herein a modified tetrazine ligation strategy based on the use of a pyrazolone coupling partner, which provides a single molecule conjugate.

Topics: Chemistry Techniques, Synthetic; Fluorescent Dyes; Heterocyclic Compounds, 1-Ring; Humans; Isomerism; Muramidase; Pyrazolones