muramidase and indole-3-carbaldehyde

The regeneration of intestinal epithelial are maintained by continuous differentiation and proliferation of intestinal stem cells (ISCs) under physiological and pathological conditions. However, little is known about the regulatory effect of intestinal microbiota on its recovery ability to repair damaged mucosal barrier. In this study, we established intestinal organoids and lamina propria lymphocytes (LPLs) co-cultured system, plus mice experiments, to explore the protective effect of Lactobacillus reuteri D8 on integrity of intestinal mucosa. We found that only live L. reuteri D8 was effective in protecting the morphology of intestinal organoids and normal proliferation of epithelial stained with EdU under TNF-α treatment, which was also further verified in mice experiments. L. reuteri D8 colonized in the intestinal mucosa and ameliorated intestinal mucosa damage caused by DSS treatment, including improvement of body weight, colon length, pathological change, and proliferation level. The repair process stimulated by L. reuteri D8 was also accompanied with increased numbers of Lgr5

Topics: Animals; Coculture Techniques; Colitis; Dextran Sulfate; Indoles; Interleukin-22; Interleukins; Intestinal Mucosa; Lactobacillus; Lymphocytes; Mice; Mice, Inbred C57BL; Mucous Membrane; Muramidase; Phosphorylation; Receptors, Aryl Hydrocarbon; Receptors, G-Protein-Coupled; Signal Transduction; STAT3 Transcription Factor; Stem Cells; Tumor Necrosis Factor-alpha