muramidase and ethyl-fumarate

Photo-crosslinkable, fumaric acid monoethyl ester-functionalized triblock oligomers are synthesized and copolymerized with N-vinyl-2-pyrrolidone to form biodegradable photo-crosslinked hydrogels. Poly(ethylene glycol) is used as the middle hydrophilic segment and the hydrophobic segments are based on D,L-lactide, trimethylene carbonate or a mixture of these monomers. Two model proteins, lysozyme and albumin, are incorporated in the hydrogels and their release is studied. The composition of the hydrophobic segments could be used to tune degradation behavior and release rates. Careful optimization of photo-polymerization conditions is needed to limit conjugation of proteins to the hydrogels and protein denaturation.

Topics: Animals; Biocompatible Materials; Cattle; Cross-Linking Reagents; Dioxanes; Drug Carriers; Fumarates; Hydrogels; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Muramidase; Photochemical Processes; Polyethylene Glycols; Polymerization; Pyrrolidinones; Serum Albumin, Bovine

Photo-crosslinked networks were prepared from fumaric acid monoethyl ester-functionalized poly(D,L-lactic acid) oligomers and N-vinyl-2-pyrrolidone. Two model proteins, lysozyme and albumin, were incorporated into the network films as solid particles and their release behavior was studied. By varying the NVP content and macromer molecular weight the degradation behavior and protein release profiles of the prepared networks could be tuned. The more hydrophilic and less densely crosslinked networks released albumin and lysozyme at a faster rate. Although active lysozyme was released from the networks over the complete release period, lysozyme release was often incomplete. This was most likely caused by electrostatic and/or hydrophobic interactions between the protein and the degrading polymer network.

Topics: Animals; Cattle; Cross-Linking Reagents; Delayed-Action Preparations; Fumarates; Gels; Glass; Hydrolysis; Lactic Acid; Light; Molecular Weight; Muramidase; Polyesters; Polymers; Pyrrolidinones; Serum Albumin, Bovine; Transition Temperature; Water