muramidase has been researched along with cyanuric-chloride* in 2 studies
2 other study(ies) available for muramidase and cyanuric-chloride
| Article | Year |
|---|---|
Preparation and use of ion-exchange chromatographic supports based on perfluoropolymers.
A poly(vinyl alcohol) (PVA) coated particulate perfluoropolymer (FEP) support has been functionalised with ion-exchange groups for use in ion-exchange chromatography of proteins. Anion-exchange (DEAE and Q) and cation-exchange (SP) groups were introduced to PVA-FEP which had previously been activated using cyanuric chloride. The equilibrium adsorption capacities of SP-PVA-FEP were 31.8 and 25.2 mg ml-1 for lysozyme and IgG respectively while for DEAE-PVA-FEP, the equilibrium adsorption capacities were 14.9 and 9.7 mg ml-1 for beta-lactoglobulin and HSA respectively. The equilibrium adsorption capacities for Q-PVA-FEP were determined to be 17.2 and 13.5 mg ml-1 for beta-lactoglobulin and HSA respectively. Experiments carried out to investigate the resolving power of materials showed that both SP and Q-PVA-FEP were able to resolve proteins with only small differences in their isoelectric points and that this resolution could be maintained at a flow-rate of 1500 cm h-1. SP-PVA-FEP was used to purify lysozyme from egg whites where a 50-fold purification, to homogeneity, was achieved in 98% yield. The anion exchanger, Q-PVA-FEP could be used to purify G6PDH from a clarified homogenate of bakers' yeast 14.3-fold in 81% yield. Topics: Adsorption; Anions; Cations; Chromatography, Ion Exchange; Egg White; Fluorine Compounds; Glucosephosphate Dehydrogenase; Ion Exchange Resins; Isoelectric Point; Molecular Structure; Muramidase; Polymers; Polyvinyl Alcohol; Saccharomyces cerevisiae; Triazines | 1997 |
A scanning tunnelling microscopy study of the formation and chemical activation of step defects on the basal plane of pyrolytic graphite.
Scanning tunnelling microscopy is used to monitor etching of the basal plane of highly orientated pyrolytic graphite by ozone, oxygen and nitric acid. These treatments are seen to produce numerous single and multilayer step defects. Subsequent modification of the graphite sheet edges flanking these cavities by cyanuric chloride, TiCl4 and other reagents is shown to activate the edges, thereby making them capable of covalently binding various molecules. Topics: Acrylates; Carbodiimides; Carbon; DNA; Graphite; Microscopy, Scanning Tunneling; Muramidase; Nitrates; Nitric Acid; Oxygen; Surface Properties; Titanium; Triazines | 1993 |