muramidase and butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanine

muramidase has been researched along with butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanine* in 1 studies

Other Studies

1 other study(ies) available for muramidase and butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanine

ArticleYear
Demonstration of receptor-mediated chemotaxis by human spermatozoa. A novel quantitative bioassay.
    Experimental cell research, 1985, Volume: 161, Issue:1

    A novel in vitro technique is described for measuring the chemotactic activity of soluble substances for human spermatozoa. This new bioassay has demonstrated that the synthetic chemotactic peptide N-formyl-Met-Leu-Phe elicits a potent, specific (i.e., receptor-mediated) chemotactic effect on human spermatozoa with an EC50 of 3.2 X 10(-10) M. Quantitative chemotactic studies on human spermatozoa with nine N-formylated-peptide analogs have shown a rank order of peptide potency indistinguishable (p less than 0.001) from that obtained in binding and chemotactic studies with rabbit neutrophils. The competitive antagonist Boc (t-butoxycarbonyl)-Phe-Leu-Phe-Leu-Phe, 10(-6) M, completely inhibited the chemotaxis elicited by f-Met-Leu-Phe, 10(-9) M, and was able to shift by one order of magnitude the molar concentration required by f-Met-Leu-Phe-Phe and f-Met-Leu-Phe to elicit the maximal response. The ability of N-formylated peptides to function as sperm chemoattractants reveals a high degree of correlation with binding, chemotaxis, and lysosomal enzyme release previously employed to define the neutrophil chemotactic receptor. This first unequivocal demonstration of substances having a receptor-mediated chemotactic effect for human male gametes suggests that human spermatozoa may indeed have the ability to respond chemotactically to appropriate environmental signals.

    Topics: Biological Assay; Chemotactic Factors; Chemotaxis; Chemotaxis, Leukocyte; Humans; Male; Muramidase; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Oligopeptides; Receptors, Formyl Peptide; Receptors, Immunologic; Spermatozoa; Structure-Activity Relationship

1985