muramidase and bromamine

muramidase has been researched along with bromamine* in 1 studies

Other Studies

1 other study(ies) available for muramidase and bromamine

ArticleYear
The role of reactive N-bromo species and radical intermediates in hypobromous acid-induced protein oxidation.
    Free radical biology & medicine, 2005, Oct-01, Volume: 39, Issue:7

    Activated eosinophils, and hypobromous acid (HOBr) generated by these cells, have been implicated in the tissue injury in asthma, allergic reactions, and some infections. Proteins are major targets for this oxidant, but limited information is available on the mechanisms of damage and intermediates formed. Reaction of HOBr with proteins is shown to result in the formation of bromamines and bromamides, from side-chain and backbone amines and amides, and 3-bromo- and 3,5-dibromo-Tyr, from Tyr residues; these materials account for ca. 70% of the oxidant consumed. Protein carbonyls, dityrosine, and 3,4-dihydroxyphenylalanine are also formed, though these are minor products (<5% of HOBr added). With BSA, extensive (selective and nonspecific) protein fragmentation and limited aggregation are also observed. The bromamines/bromamides are unstable and induce further oxidation and free radical formation as detected by EPR spin trapping. Evidence was obtained for the generation of nitrogen-centered radicals on side-chain and backbone amide groups of amino acids, peptides, and proteins. These radicals readily undergo rearrangement reactions to give carbon-centered radicals. With proteins, alpha-carbon (backbone) radicals are detected, which may play a role in protein fragmentation. A novel damage transfer pathway from Gln side-chain amide groups to backbone sites was also observed.

    Topics: Amides; Bromates; Bromides; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Electrophoresis, Polyacrylamide Gel; Free Radicals; Insulin; Muramidase; Oxidation-Reduction; Protein Structure, Quaternary; Proteins; Ribonuclease, Pancreatic; Serum Albumin, Bovine; Spin Labels; Trypsin Inhibitor, Kunitz Soybean; Tyrosine

2005