muramidase and boldine

Boldine, in low micromolar concentrations, was able to prevent brain homogenate autooxidation, the 2,2'-azobis(2-amidinopropane)(AAP)-induced lipid peroxidation of red cell plasma membranes, and the AAP-induced inactivation of lysozyme. These results are indicative of a high reactivity of boldine towards free radicals. The analysis of the boldine effect as a function of incubation times suggests that a metabolite resulting from the interaction of boldine with free radicals also exhibits antioxidant activity, being more efficient than boldine in brain homogenate auto-oxidation and less efficient in lysozyme protection experiments. This behavior may be accounted for in terms of the relative location of the scavengers needed to afford maximal protection.

Topics: Amidines; Animals; Antioxidants; Aporphines; Brain Chemistry; Dose-Response Relationship, Drug; Erythrocyte Membrane; Kinetics; Lipid Peroxidation; Luminescent Measurements; Male; Muramidase; Oxygen Consumption; Rats; Rats, Inbred Strains