muramidase and argininamide

L-arginine (Arg) is a widely used additive for suppressing protein aggregation during refolding. Systematic screening of Arg analogs provides superior additives that enhance the refolding yield more effectively than Arg. The refolding yield of hen egg lysozyme in the presence of 500 mM L-argininamide (ArgAd) increases 1.7-fold higher than Arg. Thermal unfolding experiments indicate that ArgAd has a greater denaturing effect than Arg. The refolding yield positively relates to the net charge of Arg analogs. Moreover ArgAd was also effective for the refolding of bovine carbonic anhydrase. High potency to increase the refolding yield of ArgAd compared to Arg results from high positive net charge and the denaturing property.

Topics: Animals; Arginine; Carbonic Anhydrases; Cattle; Chickens; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Molecular Structure; Muramidase; Protein Denaturation; Protein Folding; Protein Renaturation