muramidase and acetylcellulose

A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats. The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy. The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique. The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated. The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC. The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.

Topics: Aluminum Silicates; Anti-Bacterial Agents; Cellulose; Electrochemical Techniques; Enzymes, Immobilized; Escherichia coli; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; Minerals; Muramidase; Nanofibers; Photoelectron Spectroscopy; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus

Negatively charged gold nanoparticles (GNP) and positively charged lysozyme (Lys) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. The fabricated multilayer films were characterized by energy-dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), and wide-angle X-ray diffraction (XRD). Morphology of the LBL film coated mats was observed by scanning electron microscopy (SEM). Thermal degradation properties were investigated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Additionally, the result of microbial inhibition assay indicated that the composite nanofibrous mats had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, which could be used for antimicrobial packing, tissue engineering, wound dressing, etc.

Topics: Anti-Bacterial Agents; Cellulose; Dose-Response Relationship, Drug; Escherichia coli; Gold; Metal Nanoparticles; Microbial Sensitivity Tests; Muramidase; Nanofibers; Particle Size; Staphylococcus aureus; Structure-Activity Relationship; Surface Properties

Cellulose acetate nanofibrous mats were prepared by electrospunning technique. Positively charged and negatively charged composites were alternately deposited on negatively charged CA mats via layer-by-layer self-assembly technique. The morphology and inhibition rate of samples were investigated by regulating number of deposition bilayers and composition of outermost layer. Field emission scanning electron microscopy images indicated that the average diameter of fibers was increased with increasing the number of coating bilayers. Additionally, the catalytic activity of immobilized LY was measured and results showed "layer-by-layer alternative oscillation" phenomenon. The results of antibacterial assay indicated that (LY-HTCC/ALG)(10.5) films coating had the best inhibitory effect.

Topics: Adsorption; Anti-Bacterial Agents; Cellulose; Chitosan; Enzymes, Immobilized; Escherichia coli; Food Microbiology; Food Packaging; Micrococcus; Muramidase; Nanofibers; Nitrogen; Particle Size; Porosity; Quaternary Ammonium Compounds; Staphylococcus aureus; Surface Properties

To assess the use of cellulose acetate filter rods as a technique for tear collection.. The cellulose acetate rod (CR) was compared with the 'standard' glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness.. No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme (p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR (p < 0.005) and sample volume increased for stimulated collection with CR (p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR (r = -0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume (p < 0.001) and a higher protein concentration (p < 0.001) were extracted with CR.. The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.

Topics: Adult; Cellulose; Eye Proteins; Female; Humans; Immunoglobulin A, Secretory; Lactoferrin; Male; Muramidase; Specimen Handling; Tears; Young Adult