msh--4-nle-7-phe-alpha- and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene

msh--4-nle-7-phe-alpha- has been researched along with 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene* in 1 studies

Other Studies

1 other study(ies) available for msh--4-nle-7-phe-alpha- and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene

Article	Year
Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors. Journal of biomolecular screening, 2000, Volume: 5, Issue:5 High throughput screening fluorescence polarization assays using G protein-coupled receptors (GPCRs) as targets have been compared using fluorescein and BODIPY TMR-labeled peptides. The red-shifted BODIPY TMR dye exhibits improved assay performance relative to fluorescein due to improvement in both ligand affinity to the GPCRs and assay precision brought about by the higher intensity probe. Furthermore, the red-shifted dye demonstrates an insensitivity to the effects of the highly colored compound tartrazine, which can produce false-negative results for assays conducted with fluorescein as a label. Topics: alpha-MSH; Binding, Competitive; Boron Compounds; Color; Dimethyl Sulfoxide; Drug Evaluation, Preclinical; False Negative Reactions; Fluoresceins; Fluorescence Polarization; Fluorescent Dyes; Heterotrimeric GTP-Binding Proteins; Ligands; Peptides; Receptors, Cell Surface; Rhodamines; Sensitivity and Specificity; Spectrometry, Fluorescence; Tartrazine	2000

Article

Year

Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors.

Journal of biomolecular screening, 2000, Volume: 5, Issue:5

High throughput screening fluorescence polarization assays using G protein-coupled receptors (GPCRs) as targets have been compared using fluorescein and BODIPY TMR-labeled peptides. The red-shifted BODIPY TMR dye exhibits improved assay performance relative to fluorescein due to improvement in both ligand affinity to the GPCRs and assay precision brought about by the higher intensity probe. Furthermore, the red-shifted dye demonstrates an insensitivity to the effects of the highly colored compound tartrazine, which can produce false-negative results for assays conducted with fluorescein as a label.

Topics: alpha-MSH; Binding, Competitive; Boron Compounds; Color; Dimethyl Sulfoxide; Drug Evaluation, Preclinical; False Negative Reactions; Fluoresceins; Fluorescence Polarization; Fluorescent Dyes; Heterotrimeric GTP-Binding Proteins; Ligands; Peptides; Receptors, Cell Surface; Rhodamines; Sensitivity and Specificity; Spectrometry, Fluorescence; Tartrazine

2000