monomethylauristatin-f and indole

Lung fibroblasts play major roles in the lung repair/fibrosis process through synthesis and remodeling of extracellular matrix. Those aberrant activations and elevated proliferations are associated with several fibrotic lung diseases, such as idiopathic pulmonary fibrosis (IPF). Targeting fibroblasts is a promising approach for preventing aberrant remodeling of lung architecture and protect irreversible pulmonary fibrosis. In this study, we developed an aptamer that can target lung fibroblasts and explored its potential as a delivery vehicle of cytotoxic agents intracellularly. The aptamer was discovered from artificial nucleic acid libraries through cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX). This indole-modified aptamer can bind to LL97A cells, a fibroblast cell line derived from IPF patients, with high affinity (K

Topics: Aptamers, Nucleotide; Base Sequence; Cell Line; Cell Proliferation; DNA; Drug Carriers; Fibroblasts; Gene Library; Humans; Idiopathic Pulmonary Fibrosis; Indoles; Ligands; Lung; Nucleic Acids; Oligopeptides