monocrotophos and cypermethrin

Amsacta albistriga is one of the important pests of oilseed crops in India. This pest has developed high resistance to organophosphate (OP) insecticide in field. Therefore, cypermethrin insecticide was used as an alternative for this pest. After 20 generations of selection with cypermethrin, the LD50 value for A. albistriga was increased by 21.5-folds. The synergism ratio of piperonyl butoxide (PBO) and triphenyl phosphate (TPP) was increased by 10- and 9.6-fold in resistant strains and comparatively, 3.9 and 4.2-fold in susceptible strains. Detoxification enzyme analysis and native PAGE electrophoresis of esterase isoenzyme further revealed that esterase and mixed function oxidase may be involved in cypermethrin resistance in CypRes strain. In addition to enzyme analysis overexpression of CYP4M44, CYP9A77 and CYP6B47 (ortholog) can confer metabolic resistance in the CypRes strain. These data provide a foundation for further study of cypermethrin resistance mechanism observed in A. albistriga.

Topics: Animals; Esterases; Glutathione Transferase; Imidazoles; Insect Proteins; Insecticide Resistance; Insecticides; Larva; Lepidoptera; Maleates; Mixed Function Oxygenases; Monocrotophos; Neonicotinoids; Nitro Compounds; Organophosphates; Pesticide Synergists; Phylogeny; Piperonyl Butoxide; Pyrethrins

Ecotoxicological risks of agricultural application of six insecticides to soil organisms were evaluated by acute toxicity tests under laboratory condition following OECD guidelines using the epigeic earthworm Eisenia fetida as the test organism. The organochlorine insecticide endosulfan (LC(50) - 0.002 mg kg(-1)) and the carbamate insecticides aldicarb (LC(50) - 9.42 mg kg(-1)) and carbaryl (LC(50) - 14.81 mg kg(-1)) were found ecologically most dangerous because LC(50) values of these insecticides were lower than the respective recommended agricultural dose (RAD). Although E. fetida was found highly susceptible to the pyrethroid insecticide cypermethrin (LC(50) - 0.054 mg kg(-1)), the value was higher than its RAD. The organophosphate insecticides chlorpyrifos (LC(50) - 28.58 mg kg(-1)), and monocrotophos (LC(50) - 39.75 mg kg(-1)) were found less toxic and ecologically safe because the LC(50) values were much higher than their respective RAD.

Topics: Agriculture; Aldicarb; Animals; Carbaryl; Chlorpyrifos; Dose-Response Relationship, Drug; Endosulfan; Insecticides; Lethal Dose 50; Monocrotophos; Oligochaeta; Pyrethrins; Soil Pollutants

The 96 h LC(50) values of six insecticides were determined on a non-target epigeic earthworm Perionyx excavatus under laboratory conditions. Cypermethrin was found most toxic to P. excavatus (LC(50)-0.008 mg/kg), followed by endosulfan (LC(50)-0.03 mg/kg), carbaryl (LC(50)-6.07 mg/kg), chlorpyrifos (LC(50)-7.3 mg/kg), aldicarb (LC(50)-10.63 mg/kg) and monocrotophos (LC(50)-13.04 mg/kg). When these LC(50) values were compared with their respective recommended agricultural doses, aldicarb and carbaryl appeared more dangerous than other pesticides because of their lower LC(50) values than their respective recommended agricultural dose. Mean lethal time to cause 50% mortality at recommended agricultural dose (LT(50)) also indicated that aldicarb achieved the fastest LT(50) (26 h) followed by endosulfan (38 h) and carbaryl (44 h) indicating the danger of these pesticides to P. excavatus.

Topics: Agriculture; Aldicarb; Animals; Carbaryl; Chlorpyrifos; Endosulfan; Insecticides; Lethal Dose 50; Monocrotophos; Oligochaeta; Pyrethrins; Soil Pollutants

A crop protection system consisting of sequential treatments by six insecticides--dimethoate, monocrotophos, deltamethrin, endosulfan, cypermethrin, and triazophos--at recommended dosages in cotton fields in Punjab, India was investigated for its effects on nontarget soil microorganisms and their activities. Successive applications of the insecticides caused only short-lived adverse effects on the soil microorganisms. None of the insecticides used had any adverse effects on soil fungi as reflected by their total numbers. Significant change in Azotobacter numbers were observed after dimethoate, triazophos, and endosulfan treatment in 1998 soil. An increase of up to 71% in actinomycetes numbers was observed after deltamethrin treatment in the treated fields in 1995. Few short-term changes in iron-reduction capacity were observed after endosulfan and cypermethrin treatments. No adverse effect was observed on the soil respiration during all the experimental periods. The amount of residues detected in soil ranged from 8.5 to 42.0 ng g(-1)dry wt. soil for organophosphorus insecticides and from nondetectable to 5.55 ng g (-1)dry wt. soil for synthetic pyrethroids. It ranged between 7.3 and 35.6 ng g(-1)dry wt. soil for endosulfan. On many occasions two or three insecticide residues were detected together; therefore, the effect observed on soil microorganisms and their activities was a multiresidue effect. In 1998, crop soil amounts of insecticide residues were generally more than those in 1995 and 1996. Persistence and dissipation patterns in soils with a history of exposure to the insecticides compared to the non-history soils were similar.

Topics: Agriculture; Bacteria; Carbon Dioxide; Colony Count, Microbial; Dimethoate; Endosulfan; Fungi; Gossypium; Insecticides; Iron; Monocrotophos; Nitriles; Organothiophosphates; Pesticide Residues; Pyrethrins; Soil Microbiology; Soil Pollutants; Triazoles

Interaction effects of the insecticides monocrotophos and quinalphos (organophosphates), and cypermethrin (pyrethroid), on microbial activities in two agricultural soils-black vertisol soil and red alfinsol soil were tested for 30 days under laboratory conditions. Individual application of the three insecticides at 5, 10 and 25microg g(-1) to the soil distinctly enhanced the activities of cellulase and amylase. Insecticide combinations involving monocrotophos or quinalphos with cypermethrin yielded synergistic, antagonistic and additive interaction effects on both enzymes in the soils. At lower levels, 5 and 10microg g(-1), the insecticides in combination interacted additively or synergistically toward both enzymes. But, both combinations at the highest level of 25microg g(-1) exhibited an antagonistic interaction, with a reduction in enzyme activities to a level lower than that of the control. Interaction effects of insecticides in combinations on two enzyme activities in both soils were related to populations of cellulolytic and amylolytic organisms in soils under the impact of combination of insecticides. These interaction responses were persistent even for 30 days.

Topics: Amylases; Bacteria; Bacterial Proteins; Cellulase; Dose-Response Relationship, Drug; Down-Regulation; Drug Interactions; Insecticides; Monocrotophos; Organothiophosphorus Compounds; Pyrethrins; Soil; Soil Microbiology; Soil Pollutants; Time Factors; Up-Regulation

Three insecticides, monocrotophos, quinalphos, and cypermethrin, were applied at 0, 5, 10, and 25 microg g(-1) either singly or in combination to a black clay soil to investigate their effects on the soil microflora and dehydrogenase activity. All three insecticides significantly enhanced the proliferation of bacteria and fungi and the soil dehydrogenase activity even at the highest level of 25 microg g(-1). Monocrotophos or quinalphos in combination with cypermethrin at tested levels interacted significantly to yield additive, synergistic, and antagonistic responses toward bacteria and fungi and dehydrogenase activity in soil. Antagonistic interactions were more pronounced toward soil microflora and dehydrogenase activity when the two (monocrotophos or quinalphos + cypermethrin) insecticides were present together in the soil at highest level (25 + 25 microg g(-1)), whereas synergistic or additive responses occurred at lower level with the same combination of insecticides in soil.

Topics: Bacteria; Drug Interactions; Fungi; Insecticides; Monocrotophos; Organothiophosphorus Compounds; Oxidoreductases; Pyrethrins; Soil Microbiology; Stem Cells

Moth larvae (Helicoverpa armigera Hübner) collected from field crops were tested for resistance to cypermethrin, fenvalerate, endosulfan, monocrotophos and quinolphos. Larvae were treated with a dose of the pesticide that would kill 99% of the susceptible insects. The percent survival of the resistant strains was determined. Highest seasonal average percentage survival was recorded by fenvalerate (65.0%) followed by cypermethrin (62.4%). Acetylcholinesterase of resistant larvae was less sensitive to monocrotophos and methyl paraoxon. Resistant larvae showed higher activities of esterases, phosphatases and methyl paraoxon hydrolase compared with susceptible larvae. The presence of high activity of esterases was attributed to appearance of extra bands of esterases in native PAGE. The presence of P-glycoprotein expression was detected in resistant larvae using P-gp antibodies; this was not detected in the susceptible larvae. Our results indicate that the high level of resistance detected in the field pests could be because of a combined effect of decreased sensitivity to AChE, higher levels of esterases, phosphatases and the expression of P-gp.

Topics: Animals; Antibodies; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biological Assay; Electrophoresis, Polyacrylamide Gel; Endosulfan; Esterases; Hydrolases; Insecticide Resistance; Insecticides; Larva; Monocrotophos; Moths; Nitriles; Organothiophosphorus Compounds; Paraoxon; Phosphoric Monoester Hydrolases; Pyrethrins; Seasons

The fruit fly Dacus ciliatus Loew is a pest of the fruits of many cucurbit species. We studied the effect of organaophosphate and pyrethroid compounds on the adult flies by using surface contact and oral administration. In contrast to other fruit flies, we found that organophosphates were ineffective against D. ciliatus. This was supported by the insignificant decrease of head acetylcholinesterase activity. All tested pyrethroids showed satisfactory killing ability, rapid and massive knockdown effect, and prevention of oviposition. Piperonyl butoxide considerably increased the toxicity of pyrethroids, which can be explained by oxidase detoxification of these compounds in D. ciliatus. It can be concluded that pyrethroids have high potential for controlling D. ciliatus.

Topics: Acetylcholinesterase; Animals; Biological Assay; Dimethoate; Diptera; Female; Fertility; Insect Control; Insecticides; Malathion; Male; Monocrotophos; Organothiophosphorus Compounds; Phosphoramides; Pyrethrins

Topics: Azospirillum; Bacillus; Biodegradation, Environmental; Insecticides; Monocrotophos; Nitriles; Organothiophosphorus Compounds; Pesticide Residues; Pyrethrins; Soil Microbiology