monensin and cinnamaldehyde

Topics: Acrolein; Animal Feed; Animals; Blood Urea Nitrogen; Body Weight; Cattle; Diet; Female; Monensin; Weaning

This study was conducted to evaluate the effect of dietary addition of cinnamon oil (CIN), cinnamaldehyde (CDH), or monensin (MON) on enteric methane (CH4) emission in dairy cows. Eight multiparous lactating Holstein cows fitted with ruminal cannulas were used in a replicated 4×4 Latin square design (28-day periods). Cows were fed (ad libitum) a total mixed ration ((TMR); 60 : 40 forage : concentrate ratio, on a dry matter (DM) basis) not supplemented (CTL), or supplemented with CIN (50 mg/kg DM intake), CDH (50 mg/kg DM intake), or monensin (24 mg/kg of DM intake). Dry matter intake (DMI), nutrient digestibility, N retention, and milk performance were measured over 6 consecutive days. Ruminal degradability of the basal diet (with no additive) was assessed using in sacco incubations (0, 2, 4, 8, 16, 24, 48, 72 and 96 h). Ruminal fermentation characteristics (pH, volatile fatty acids (VFA), and ammonia (NH3)) and protozoa were determined over 2 days. Enteric CH4 emissions were measured over 6 consecutive days using the sulfur hexafluoride (SF6) tracer gas technique. Adding CIN, CDH or MON to the diet had no effects on DMI, N retention, in sacco ruminal degradation and nutrient digestibility of the diet. Ruminal fermentation characteristics and protozoa numbers were not modified by including the feed additives in the diet. Enteric CH4 emission and CH4 energy losses averaged 491 g/day and 6.59% of gross energy intake, respectively, and were not affected by adding CIN, CDH or MON to the diet. Results of this study indicate that CIN, CDH and MON are not viable CH4 mitigation strategies in dairy cows.

Topics: Acrolein; Ammonia; Animals; Cattle; Cinnamomum zeylanicum; Dairying; Diet; Dietary Supplements; Digestion; Energy Intake; Fatty Acids, Volatile; Female; Fermentation; Hydrogen-Ion Concentration; Lactation; Methane; Milk; Monensin; Parity; Plant Oils; Rumen

Cinnamaldehyde (CIN) is the main active component of cinnamon (Cinnamomum cassia) oil and has been tested as alternative feed additive in cattle production. Little information was available on the effect of dietary CIN in comparison to monensin (MO) on beef fatty acid (FA) profile. This study analyzed FA profiles of liver, subcutaneous fat and pars costalis diaphragmatis (PCD) muscle obtained from steers (n = 70) finished on diets: control, a barley grain-silage feedlot diet; 330 mg/head.day MO; and 400, 800 or 1600 mg/head.day CIN treatments.. Inclusion of MO or CIN did not affect total saturated, unsaturated, polyunsaturated FA and individual FA in the various tissues with exceptions that proportion of palmitic acid in PCD muscle was increased by 800 mg/steer.day CIN (P < 0.05). There were positive correlations (P < 0.05) on oleic, linoleic, conjugated linoleic acid (CLA)-c9,t11 and 18:1-t10 between the subcutaneous fat and PCD muscle, and on α-linolenic acid, CLA-c9,t11 and 18:1-t10 between PCD muscle and liver, whereas correlations on the FA between the subcutaneous fat and liver were not significant except for 18:1-t10 (P < 0.01).. The results indicate that the supplementation of CIN and MO to feedlot diet has limited effect on beef FA profiles.

Topics: Acrolein; alpha-Linolenic Acid; Animal Feed; Animals; Cattle; Diet; Dietary Supplements; Fatty Acids; Humans; Linoleic Acids, Conjugated; Liver; Meat; Monensin; Muscles; Subcutaneous Fat

Two representative strains of Gram-negative rumen bacteria from the genus Prevotella were used as model organisms in order to evaluate the effect of cinnamaldehyde (the secondary metabolite found in extracts of the Cinnamomum family) vs. sodium monensin on growth, cell size and cell protein production. Prevotella bryantii B(1)4 was found to be remarkably more resistant to the action of both compounds than Prevotella ruminicola 23. The approximate IC(50) concentrations of sodium monensin influenced the increase in cell size of both strains during growth, which was much more pronounced in the case of the B(1)4 strain. A similar effect was observed in strain B(1)4 when 1.438 mmol/L cinnamaldehyde was added to the growth medium, indicating a possible interference with cell division. The action of cinnamaldehyde on P. bryantii B(1)4 was concentration-dependent, in contrast to the effect observed on P. ruminicola 23.

Topics: Acrolein; Animals; Antiprotozoal Agents; Inhibitory Concentration 50; Monensin; Parasitic Sensitivity Tests; Phenotype; Prevotella; Prevotella ruminicola; Rumen

An in vitro study in dual-flow continuous-culture fermentors was conducted with two different concentrations of monensin, cinnamaldehyde or garlic extract added to 1:1 forage-to-concentrate diet in order to determine their effects on selected rumen bacterial populations. Samples were subjected to total DNA extraction, restriction analysis of PCR amplified parts of 16S rRNA genes (ARDRA) and subsequent analysis of the restriction profiles by lab-on-chip technology with the Agilent's Bioanalyser 2100. Eub338-BacPre primer pair was used to select for the bacteria from the genera Bacteroides, Porphyromonas and Prevotella, especially the latter representing the dominant Gram-negative bacterial population in the rumen. Preliminary results of HaeIII restriction analysis show that the effects of monensin, cinnamaldehyde and garlic extract on the BacPre targeted ruminal bacteria are somewhat different in regard to targeted populations and to the nature of the effect. Garlic extract was found to trigger the most intensive changes in the structure of the BacPre targeted population. Comparison of the in silico restriction analysis of BacPre sequences deposited in different DNA databanks and of the results of performed amplified ribosomal DNA restriction analysis showed differences between the predicted and obtained HaeIII restriction profiles, and suggested the presence of novel, still unknown Prevotella populations in studied samples.

Topics: Acrolein; Animals; Anti-Bacterial Agents; Bacteria; Bacteroides; Deoxyribonucleases, Type II Site-Specific; DNA Fingerprinting; DNA, Bacterial; DNA, Ribosomal; Garlic; Genes, Bacterial; Genes, rRNA; Monensin; Plant Extracts; Porphyromonas; Prevotella; Ribotyping; RNA, Ribosomal, 16S; Rumen