miyabenol-c and epsilon-viniferin

Cell entry of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) depends on specific host cell proteases, which are the key targets for preventing and treating viral infections. Herein, we describe miyabenol C and trans-ε-viniferin, two resveratrol oligomers that specifically inhibit SARS-CoV-2 entry by targeting host protease cathepsin L. Several cell-based assays were used to demonstrate the effect of resveratrol oligomers, and their target was identified via screening of antiviral targets. Molecular docking analysis suggested that the oligomers could occupy the active cavity of cathepsin L. The surface plasmon resonance assay showed that the equilibrium dissociation constant (

Topics: Cathepsin L; COVID-19; Humans; Molecular Docking Simulation; Resveratrol; SARS-CoV-2; Virus Internalization

The currently accepted paradigm is that fruits and vegetables should be consumed fresh and that their quality deteriorates during storage; however, there are indications that some metabolic properties can, in fact, be improved. We examined the effects of low temperature and high-CO

Topics: Acetates; Anaerobiosis; Benzofurans; Carbon Dioxide; Cold Temperature; Food Storage; Metabolomics; Pyruvic Acid; Signal Transduction; Stilbenes; Transcription Factors; Transcriptome; Up-Regulation; Vitis

Two new oligostilbenes, parthenocissins M (1) and N (2), together with two known compounds, miyabenol C (3) and ϵ-viniferin (4), were isolated from the stem of Parthenocissus quinquefolia. Their structures were elucidated by means of NMR, UV, IR, and MS data.

Topics: Benzofurans; Drugs, Chinese Herbal; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Plant Stems; Stilbenes; Vitaceae

An activity-guided isolation of bioactive stilbenes has been carried out with the grapevine-shoot extract Vineatrol 30. After hexane precipitation of the polymeric constituents, the stilbene mixture was separated on a preparative scale using low-speed rotary countercurrent chromatography (LSRCCC). The antiproliferative activity of the separated LSRCCC fractions was then screened in the human cancer cell line A-431, and trans-resveratrol, trans-ε-viniferin, r-2-viniferin, hopeaphenol, and miyabenol C were identified as active principles. In addition, a new class of stilbene derivatives, which exhibit a γ-lactam ring structure and exert a weak growth-inhibiting activity in A-431 cells, has been identified.

Topics: Antineoplastic Agents, Phytogenic; Benzofurans; Cell Line, Tumor; Chemical Fractionation; Countercurrent Distribution; Drug Screening Assays, Antitumor; Humans; Molecular Structure; Phenols; Plant Shoots; Resveratrol; Stilbenes; Vitis