mitragynine and methyl-jasmonate

Mitragynine is a pharmacologically-active terpenoid indole alkaloid found in Mitragyna speciosa leaves. Treatment with methyl jasmonate (10 μM) for 24 h and yeast extract (0.1 mg/ml) for 12 h were the optimum conditions of elicitation of mitragynine accumulation in a M. speciosa shoot culture. The former elicitor gave 0.11 mg mitragynine/g dry wt. Tryptophan decarboxylase and strictosidine synthase mRNA levels were enhanced in accordance with mitragynine accumulation.

Topics: Acetates; Aromatic-L-Amino-Acid Decarboxylases; Carbon-Nitrogen Lyases; Culture Media; Culture Techniques; Cyclopentanes; Gene Expression Regulation, Plant; Mitragyna; Oxylipins; Plant Proteins; Plant Shoots; RNA, Messenger; Secologanin Tryptamine Alkaloids; Yeasts