mitosox-red and 4-hydroxy-2-nonenal

mitosox-red has been researched along with 4-hydroxy-2-nonenal* in 1 studies

Other Studies

1 other study(ies) available for mitosox-red and 4-hydroxy-2-nonenal

ArticleYear
Boronate probes for the detection of hydrogen peroxide release from human spermatozoa.
    Free radical biology & medicine, 2015, Volume: 81

    Human spermatozoa are compromised by production of reactive oxygen species (ROS), and detection of ROS in spermatozoa is important for the diagnosis of male infertility. The probes 2',7'-dichlorohydrofluorescein diacetate (DCFH), dihydroethidium (DHE), and MitoSOX red (MSR) are commonly used for detecting ROS by flow cytometry; however, these probes lack sensitivity to hydrogen peroxide (H2O2), which is particularly damaging to mammalian sperm cells. This study reports the synthesis and use of three aryl boronate probes, peroxyfluor-1 (PF1), carboxyperoxyfluor-1, and a novel probe, 2-(2-ethoxyethoxy)ethoxyperoxyfluor-1 (EEPF1), in human spermatozoa. PF1 and EEPF1 were effective at detecting H2O2 and peroxynitrite (ONOO(-)) produced by spermatozoa when stimulated with menadione or 4-hydroxynonenal. EEPF1 was more effective at detection of ROS in spermatozoa than DCFH, DHE, or MSR; furthermore it distinguished poorly motile sperm as shown by greater ROS production. EEPF1 should therefore have a significant role in the diagnosis of oxidative stress in male infertility, cryopreservation, age, lifestyle, and exposure to environmental toxicants.

    Topics: Aldehydes; Boronic Acids; Cells, Cultured; Ethidium; Fluoresceins; Fluorescent Dyes; Humans; Hydrogen Peroxide; Male; Molecular Probes; Organophosphorus Compounds; Peroxynitrous Acid; Phenanthridines; Sperm Motility; Spermatozoa; Vitamin K 3

2015