methylcellulose and 2--3--dideoxycytidine-5--triphosphate

methylcellulose has been researched along with 2--3--dideoxycytidine-5--triphosphate* in 1 studies

Other Studies

1 other study(ies) available for methylcellulose and 2--3--dideoxycytidine-5--triphosphate

Article	Year
Labeling of double-stranded DNA by ROX-dideoxycytosine triphosphate using terminal deoxynucleotidyl transferase and separation by capillary electrophoresis. Analytical chemistry, 1994, Dec-01, Volume: 66, Issue:23 Terminal transferase is used to add a single fluorescently labeled dideoxynucleotide to double-stranded DNA prepared by restriction endonuclease action on a bacteriophage. The product is separated by capillary electrophoresis with both hydroxypropylmethylcellulose and non-cross-linked polyacrylamide. The reaction products generate single peaks for each fragment with hydroxypropylmethylcellulose. However, the higher resolution separation produced by non-cross-linked polyacrylamide shows that the product contains two components for each restriction digest fragment. This labeling technique should be useful in restriction fragment length polymorphism studies. Topics: Deoxycytosine Nucleotides; Dideoxynucleotides; DNA; DNA Nucleotidylexotransferase; DNA-Binding Proteins; Electrophoresis; Fluorescent Dyes; Hypromellose Derivatives; Methylcellulose; Polymorphism, Genetic; Spectrometry, Fluorescence	1994

Article

Year

Labeling of double-stranded DNA by ROX-dideoxycytosine triphosphate using terminal deoxynucleotidyl transferase and separation by capillary electrophoresis.

Analytical chemistry, 1994, Dec-01, Volume: 66, Issue:23

Terminal transferase is used to add a single fluorescently labeled dideoxynucleotide to double-stranded DNA prepared by restriction endonuclease action on a bacteriophage. The product is separated by capillary electrophoresis with both hydroxypropylmethylcellulose and non-cross-linked polyacrylamide. The reaction products generate single peaks for each fragment with hydroxypropylmethylcellulose. However, the higher resolution separation produced by non-cross-linked polyacrylamide shows that the product contains two components for each restriction digest fragment. This labeling technique should be useful in restriction fragment length polymorphism studies.

Topics: Deoxycytosine Nucleotides; Dideoxynucleotides; DNA; DNA Nucleotidylexotransferase; DNA-Binding Proteins; Electrophoresis; Fluorescent Dyes; Hypromellose Derivatives; Methylcellulose; Polymorphism, Genetic; Spectrometry, Fluorescence

1994