methyl-jasmonate and zeatin-riboside

Adventitious root (AR) formation is a bottleneck for the mass propagation of apple rootstocks, and water stress severely restricts it. Different hormones and sugar signaling pathways in apple clones determine AR formation under water stress, but these are not entirely understood. To identify them, GL-3 stem cuttings were cultured on polyethylene glycol (PEG) treatment. The AR formation was dramatically decreased compared with the PEG-free control (CK) cuttings by increasing the endogenous contents of abscisic acid (ABA), zeatin riboside (ZR), and methyl jasmonate (JA-me) and reducing the indole-3-acetic acid (IAA) and gibberellic acid 3 (GA3) contents. We performed a transcriptomic analysis to identify the responses behind the phenotype. A total of 3204 differentially expressed genes (DEGs) were identified between CK and PEG, with 1702 upregulated and 1502 downregulated genes. Investigation revealed that approximately 312 DEGs were strongly enriched in hormone signaling, sugar metabolism, root development, and cell cycle-related pathways. Thus, they were selected for their possible involvement in adventitious rooting. However, the higher accumulation of ABA, ZR, and JA-me contents and the upregulation of their related genes, as well as the downregulation of sugar metabolism-related genes, lead to the inhibition of ARs. These results indicate that AR formation is a complicated biological process chiefly influenced by multiple hormonal signaling pathways and sugar metabolism. This is the first study to demonstrate how PEG inhibits AR formation in apple plants.

Topics: Abscisic Acid; Acetates; Cyclopentanes; Dehydration; Gene Expression Profiling; Gene Expression Regulation, Plant; Gibberellins; Indoleacetic Acids; Isopentenyladenosine; Malus; Oxylipins; Plant Proteins; Plant Roots; Polyethylene Glycols; Sequence Analysis, RNA

The characterization of yield trait mutants is important for understanding the regulation of grain yield formation in staple food crops. Meh0239 is a yield trait-related mutant identified from a mutant library of the common wheat cultivar Wangshuibai created by ethylmethyl sulfide (EMS) treatment of dry seeds. To shed some light on the nature of this mutation, it was investigated morphologically, physiologically, anatomically and genetically. The mutant plant showed obvious phenotypic differences in comparison with the wild type, starting at the seedling stage, including reduced plant height, wider and shorter leaves, shortened spikes, spikelets and grains and a more compact spikelet distribution. Also, seeds produced in the mutant germinated more slowly. Meh0239 contained a significantly higher level of abscisic acid (ABA) but lower levels of indole-3-acetic acid (IAA), methyl jasmonate (MeJA) and zeatin riboside (ZR) in flag leaves. Cells of all types in the leaf epidermis appeared shorter along the axial direction. The bulliform cells and long cells on the adaxial leaf surface were abnormal in shape. A genetic analysis using two F₂ segregating populations indicated that a single recessive mutation in wheat chromosome 7DS, about 3.1cM distal from Xwmc506, caused these variations. Because of the pleiotropic nature of this gene and its relation with yield trait formation, we named it Yt1 for yield trait related 1.

Topics: Abscisic Acid; Acetates; Cyclopentanes; Gene Expression Regulation, Plant; Genes, Plant; Genes, Recessive; Germination; Indoleacetic Acids; Isopentenyladenosine; Mutation; Oxylipins; Phenotype; Plant Leaves; Plants, Genetically Modified; Seeds; Triticum