methyl-jasmonate has been researched along with 1-naphthaleneacetic-acid* in 2 studies
2 other study(ies) available for methyl-jasmonate and 1-naphthaleneacetic-acid
| Article | Year |
|---|---|
NtPDR3, an iron-deficiency inducible ABC transporter in Nicotiana tabacum.
In plants, the ABC transporter PDR (pleiotropic drug resistance) subfamily is composed of approximately 15 genes, few of which have been analyzed. We have identified NtPDR3, a Nicotiana tabacum PDR gene belonging to a cluster for which no functional data was previously available. NtPDR3 was found to be induced in suspension cells treated with methyl jasmonate, salicylic acid, 1-naphthalene acetic acid, or cembrene, a macrocyclic diterpene. In agreement with the identification of a putative iron deficiency element in the NtPDR3 transcription promoter region, we found that iron deficiency in the culture medium induced NtPDR3 expression, thus suggesting a new function of the PDR transporter family. Topics: Acetates; ATP-Binding Cassette Transporters; Base Sequence; Biological Transport; Cells, Cultured; Chelating Agents; Cloning, Molecular; Consensus Sequence; Culture Media; Cyclopentanes; Diterpenes; DNA, Complementary; Egtazic Acid; Gene Expression Regulation, Plant; Genes, Plant; Genetic Vectors; Iron Deficiencies; Molecular Sequence Data; Naphthaleneacetic Acids; Nicotiana; Oxylipins; Phylogeny; Plant Growth Regulators; Plant Proteins; Promoter Regions, Genetic; Salicylic Acid; Transcription, Genetic | 2005 |
Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: cloning, characterization and differential expression in tissues and by stress compounds.
Camptothecin derivatives are clinically used anti-tumor compounds that biogenetically belong to a group of monoterpenoid indole alkaloids (TIA). We have already established a hairy root culture of Ophiorrhiza pumila (Rubiaceae) that produces camptothecin. The present study describes the cloning and characterization of cDNAs encoding strictosidine synthase (OpSTR; EC 4.3.3.2) and tryptophan decarboxylase (OpTDC; EC 4.1.1.28), two key enzymes in the biosynthesis of TIA from hairy roots of O. pumila. We also isolated the cDNA coding for NADPH:cytochrome P450 reductase (OpCPR; EC 1.6.2.4) that is presumed to be indirectly involved in camptothecin synthesis. The recombinant OpSTR and OpTDC proteins exhibit STR and TDC activities, respectively, when expressed in Escherichia coli. The tissue-specific and stress-inducible expression patterns of OpSTR and OpTDC were quite similar, unlike those of OpCPR. The high expression of OpSTR and OpTDC observed in hairy roots, roots and stems were closely correlated with STR protein accumulation as observed by immunoblot analysis. Plant stress compounds like salicylic acid repressed expression of OpSTR and OpTDC, suggesting coordinate regulation of these genes for camptothecin biosynthesis. Topics: Acetates; Adenine; Aromatic-L-Amino-Acid Decarboxylases; Base Sequence; Benzyl Compounds; Camptothecin; Carbon-Nitrogen Lyases; Cloning, Molecular; Cyclopentanes; DNA, Complementary; Escherichia coli; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Kinetin; Molecular Sequence Data; NADPH-Ferrihemoprotein Reductase; Naphthaleneacetic Acids; Oxylipins; Phylogeny; Plant Roots; Purines; Rubiaceae; Salicylic Acid; Sequence Analysis, DNA | 2003 |