methyl-13-hydroperoxy-9-11-octadecadienoate and diphenyl-1-pyrenylphosphine

Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.

Topics: Animals; Cell Membrane; Female; Fluorescent Dyes; Free Radicals; Hydrogen Peroxide; Lipid Peroxidation; Lipid Peroxides; Liposomes; Mice; Mice, Inbred ICR; Microscopy, Fluorescence; Neutrophils; Organophosphorus Compounds; Oxidants; Oxidation-Reduction; Phosphatidylcholines; Pyrenes; Solutions; Spectrometry, Fluorescence; tert-Butylhydroperoxide; Tetradecanoylphorbol Acetate