methyl-13-hydroperoxy-9-11-octadecadienoate and 2-2--azobis(2-amidinopropane)

Methyl linoleate hydroperoxides (MLHPO) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), a widely used free radical initiator, were examined for effects on antibody production by spleen cells using plaque-forming cell response against sheep red blood cells (SRBC). The in vitro primary antibody response was enhanced in the presence of MLHPO at a concentration range of 2-20 microM or AAPH at 0.1 microM, but was suppressed with higher concentrations of these compounds. In the in vitro secondary antibody responses, both MLHPO and AAPH failed to increase plaque-forming cell response above that of the control culture. Following the oral administration of MLHPO (2.29 g/kg) four times to mice, in vivo primary plaque-forming cell response was significantly suppressed. After, a single intraperitoneal injection of AAPH (60 mg/kg) to mice, in vitro primary plaque-forming cell response was also suppressed. These findings suggest that primary antibody response can be affected by lipid hydroperoxides and oxygen radicals in vivo.

Topics: Amidines; Animals; Antibody Formation; Lipid Peroxides; Male; Mice; Mice, Inbred C57BL; Mutagens; Spleen