methotrexate and nadp
methotrexate has been researched along with nadp in 163 studies
Research
Studies (163)
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 101 (61.96) | 18.7374 |
| 1990's | 35 (21.47) | 18.2507 |
| 2000's | 17 (10.43) | 29.6817 |
| 2010's | 7 (4.29) | 24.3611 |
| 2020's | 3 (1.84) | 2.80 |
Authors
| Authors | Studies |
|---|---|
| Adams, J; Evenson, DA; McIvor, RS; Wagner, CR | 1 |
| Huennekens, FM; Neef, VG | 2 |
| Harrap, KR; Hart, LI; Jackson, RC | 1 |
| Bjur, RA; Burgen, AS; Dann, JG; King, RW; Ostler, G; Roberts, GC; Scudder, P; Turner, PC | 1 |
| Acar, J; Burchall, JJ; Goldstein, FW; Harvey, RJ; Pattishall, KH | 1 |
| Blakley, RL; Cocco, L; London, RE; Matwiyoff, NA; Walker, TE | 2 |
| Alden, RA; Bolin, JT; Freer, ST; Hamlin, R; Hoogsteen, K; Kraut, J; Matthews, DA; Poe, M; Williams, M; Xuong, N | 1 |
| Bertino, JR; Greenfield, NJ; Gupta, SV; Makulu, DR; Moroson, BA; Poe, M; Williams, MN | 1 |
| Dunlap, RB; Lee, TK; Williams, TJ | 1 |
| Rao, NA; Reddy, VA | 1 |
| Henkens, RW; Kitchell, BB | 1 |
| Kaufman, BT; Subramanian, S | 1 |
| Alden, RA; Bolin, JT; Filman, DJ; Freer, ST; Hamlin, R; Hol, WG; Kisliuk, RL; Kraut, J; Matthews, DA; Pastore, EJ; Plante, LT; Xuong, N | 1 |
| Matthews, DA | 1 |
| Bayley, PM; Hood, K; Roberts, GC | 1 |
| Duggleby, RG; Morrison, JF; Williams, JW | 1 |
| Williams, MN | 1 |
| Birdsall, B; Burgen, AS; Feeney, J; Roberts, GC; Way, JL | 1 |
| Greenfield, NJ | 2 |
| Ciszak, E; Cody, V; Freisheim, JH; Kalman, TI; Luft, JR | 1 |
| Birdsall, B; Cheung, HT; Feeney, J | 1 |
| Bauer, CJ; Birdsall, B; Feeney, J; Gerothanassis, IP | 1 |
| Bauer, CJ; Birdsall, B; Carr, MD; Feeney, J; Frenkiel, TA; Jimenez-Barbero, J; McCormick, JE; Polshakov, VI; Roberts, GC | 1 |
| Brown, KA; Farnum, MF; Howell, EE; Kraut, J; Warren, MS | 1 |
| Freisheim, JH; Huang, SM; Ratnam, M; Tan, XH; Thompson, PD | 1 |
| Ashford, VA; Davies, JF; Delcamp, TJ; Freisheim, JH; Kraut, J; Prendergast, NJ | 1 |
| Birdsall, B; Feeney, J; Hammond, SJ; Roberts, GC; Tendler, SJ | 1 |
| Danziger, DJ; Dean, PM | 1 |
| Carpano, P; Cercignani, G; Degan, P; Montagnoli, G | 1 |
| Appleman, JR; Blakley, RL; Delcamp, TJ; Freisheim, JH; Prendergast, NJ | 1 |
| Allegra, CJ; Chabner, BA; Drake, JC; Kovacs, JA; Masur, H; Swan, JC | 1 |
| Absil, J; Pictet, R; Stone, SR; Thillet, J | 1 |
| Baccanari, DP; Delcamp, TJ; Forsch, RA; Freisheim, JH; Rosowsky, A; Solan, VC | 1 |
| Roberts, GC | 1 |
| Briand, CM; Gilli, RM; Sari, JC; Sica, LM | 1 |
| Morrison, JF; Stone, SR | 1 |
| Chen, GX; Mueller, C; Wendlinger, M; Zolg, JW | 1 |
| Komatsu, K; Nakagawa, S; Nakamura, H; Umeyama, H | 1 |
| Bourdeaux, M; Briand, C; Gilli, R; Sari, JC; Sica, L | 1 |
| Allegra, CJ; Chabner, BA; Drake, JC | 1 |
| Abelson, HT; Dolnick, BJ; Kamen, BA; Nylen, PA; Peterson, DW; Whitehead, VM | 1 |
| Huennekens, FM; Jackson, RC | 1 |
| Greenfield, NJ; Hoogsteen, K; Poe, M; Williams, MN | 1 |
| Sirotnak, FM | 1 |
| Greenfield, NJ; Hirshfield, JM; Hoogsteen, K; Poe, M; Williams, MN | 2 |
| Greenfield, NJ; Hoogsteen, K; Williams, MN | 1 |
| Cotton, DW; van Rossum, E | 1 |
| Greenfield, NJ; Poe, M; Williams, MN | 1 |
| Dunlap, RB; Liu, JK | 1 |
| Kaplan, NO; Kisliuk, RL; Pastore, EJ; Plante, LT; Wright, JM | 1 |
| Schaefer, H; Schalla, W; Zesch, A | 1 |
| Gawthorne, JM; Smith, RM | 1 |
| DiGirolamo, PM; Fujii, K; Henderson, GB; Huennekens, FM; Jacobsen, DW; Neef, VG; Rader, JI | 1 |
| Burchall, JJ; Hitchings, GH | 2 |
| Albrecht, AM; Hutchison, DJ; Palmer, JL | 1 |
| Bertino, JR; Perkins, JP | 1 |
| Roberts, D | 1 |
| Jaffe, JJ; McCormack, JJ | 1 |
| Bertino, JR; Hillcoat, BL; Perkins, JP | 1 |
| Grignani, F; Martelli, MF; Tonato, M | 1 |
| Hakala, MT | 1 |
| Albrecht, AM; Hutchison, DJ; Pearce, FK | 1 |
| Neal, GE; Stanley, BG; Williams, DC | 1 |
| Gutteridge, WE; Jaffe, JJ; McCormack, JJ | 1 |
| Albrecht, AM; Hutchison, DJ | 1 |
| Crosby, LK; Kozloff, LM; Lute, M; Verses, C | 1 |
| Bertino, JR; Cashmore, AR; Hillcoat, BL | 1 |
| Bachur, NR; Jarabak, J | 1 |
| Dunlap, RB; Gundersen, LE; Huennekens, FM | 1 |
| Harding, NG; Newbold, PC | 1 |
| Pastore, EJ | 1 |
| Dunlap, RB; Freisheim, JH; Gundersen, LE; Harding, NG; Huennekens, FM; Otting, F | 1 |
| Hutchison, DJ; Rader, JI | 1 |
| Gauldie, J; Hillcoat, BL | 1 |
| Albrecht, AM; Biedler, JL; Hutchison, DJ | 1 |
| Bertino, JR; McCullough, JL | 1 |
| D'Souza, L; Freisheim, JH | 1 |
| Huennekens, FM; Otting, F | 1 |
| Erickson, JS; Mathews, CK | 1 |
| Lippens, RJ | 1 |
| Bolard, J; Seng, G | 1 |
| Clore, GM; Gronenborn, AM; Papadopoulos, P | 1 |
| Birdsall, B; Clore, GM; Feeney, J; Gronenborn, AM; Roberts, GC | 1 |
| Anderson, LA; Goldman, ID; Matherly, LH | 1 |
| Dunn, SM; King, RW | 1 |
| Birdsall, B; Burgen, AS; Feeney, J; Gronenborn, A; Kaptein, R; Roberts, GC | 1 |
| Dwivedi, CM; Kisliuk, RL; Pastore, EJ; Plante, LT; Verma, SP; Wallach, DF | 1 |
| Bertino, JR; Kamen, BA; Whyte-Bauer, W | 1 |
| Fan, CC; Huennekens, FM; Vitols, KS | 1 |
| Cayley, PJ; Dunn, SM; King, RW | 1 |
| Baccanari, DP; Joyner, SS | 1 |
| Bush, K; Freudenberger, JS; Meyers, E; Slusarchyk, DS; Sykes, RB | 1 |
| Blakley, RL; Cocco, L; Groff, JP; London, RE | 1 |
| Scheufler, E | 1 |
| Brown, LF; Feld, RD; Johnson, GF; Witte, DL | 1 |
| Da Costa, M; Iqbal, MP; Rothenberg, SP | 1 |
| Boles, JO; Dunlap, RB; Lebioda, L; Odom, JD | 1 |
| Briand, C; Gilli, R; Makarov, A; Protasevich, I; Sasso, S | 1 |
| Meiering, EM; Wagner, G | 1 |
| Delcamp, TJ; Freisheim, JH; Li, H; Meiering, EM; Wagner, G | 1 |
| Appleman, JR; Blakley, RL; Chunduru, SK; Cody, V; Galitsky, N; Lewis, WS; Luft, JR; Pangborn, W; Spencer, HT | 1 |
| Benkovic, SJ; Huang, Z; Wagner, CR | 1 |
| Andrews, J; Arnold, JR; Basran, J; Birdsall, B; Feeney, J; Roberts, GC; Thomas, JA | 1 |
| Barrie, PJ; Birdsall, B; Feeney, J; Gerothanassis, IP | 1 |
| Briand, CM; Gilli, RM; Rimet, OS; Sari, JC; Sasso, SP | 1 |
| Appleman, JR; Blakley, RL; Nakano, T; Spencer, HT | 1 |
| Appleman, JR; Blakley, RL; Chunduru, SK; Cody, V; Luft, JR; Pangborn, W | 1 |
| Frieden, C; Hoeltzli, SD | 1 |
| Falzone, CJ; Iwakura, M; Jones, BE; Matthews, CR | 1 |
| Bertino, JR; Dicker, AP; Otter, GM; Schmid, FA; Schweitzer, BI; Sirotnak, FM; Volkenandt, M; Waltham, MC | 1 |
| Benkovic, SJ; Falzone, CJ; Wright, PE | 1 |
| Briand, C; Gilli, R; Lopez, C; Sari, JC; Sasso, S | 1 |
| Allegra, CJ; Chu, E; Grem, JL; Takimoto, CH; Voeller, D | 1 |
| Del Carpio, CA; Sasaki, S; Takahashi, Y | 1 |
| Giovanelli, J; Kaufman, S; Witteveen, CF | 1 |
| Huennekens, FM | 1 |
| Birdsall, B; Feeney, J; Frenkiel, TA; Gargaro, AR; Morgan, WD; Nieto, PM | 1 |
| Caves, LS; Hubbard, RE; Roberts, GC; Verma, CS | 1 |
| Johnson, JM; Meiering, EM; Pardo, J; Rosowsky, A; Wagner, G; Wright, JE | 1 |
| Beverley, SM; Hardy, LW; Nare, B | 1 |
| Blakley, RL; Cody, V; Galitsky, N; Gangjee, A; Luft, JR; Pangborn, W | 1 |
| Callender, R; Chen, YQ; Cheung, HT; Gulotta, M | 1 |
| Bray, T; Chang, CF; Whiteley, JM | 1 |
| Auerbach, G; Bader, G; Dams, T; Huber, R; Jacob, U; Jaenicke, R; Ploom, T | 1 |
| Birdsall, B; Feeney, J; Polshakov, VI | 1 |
| Bowen, D; Pitts, C; Southerland, WM | 1 |
| Beverley, SM; Gourley, DG; Hardy, LW; Hunter, WN; Leonard, GA; Luba, J; Schüttelkopf, AW | 1 |
| Robert Matthews, C; Wallace, LA | 1 |
| Matthews, CR; O'Neill, JC; Svensson, AK | 1 |
| Alvarez, JC; Belmarsh, M; Joseph-McCarthy, D; Moustakas, D; Thomas, BE | 1 |
| Alvarez, JC; Joseph-McCarthy, D | 1 |
| Feller, G; Gerday, C; Glansdorff, N; Xu, Y | 1 |
| MISRA, DK | 1 |
| BERTINO, JR; BIEBER, AL; BOOTH, BA; CASHMORE, A; SARTORELLI, AC | 1 |
| WILLIAMS, DC | 1 |
| GREENBERG, DM; MORALES, DR | 1 |
| DONATI, GJ; HUTCHISON, DJ; SIROTNAK, FM | 1 |
| KENNEDY, EP; LINDBERG, M; TIETZ, A | 1 |
| CRAWFORD, EJ; FRIEDKIN, M; GOLDIN, A; HUMPHREYS, SR; KASHKET, ER | 1 |
| MAINWARING, WI; WILLIAMS, DC | 1 |
| Balog, E; Becker, T; Daniel, R; Finney, J; Lechner, R; Oettl, M; Smith, JC | 1 |
| Avery, MA; Carvalho, P; Gibellini, F; Hunter, WN; McLuskey, K | 1 |
| Cody, V; Luft, JR; Pangborn, W | 1 |
| Abali, EE; Banerjee, D; Bertino, JR; Menon, LG; Mishra, PJ; Peters, R; Skacel, N | 1 |
| Ainavarapu, SR; Badilla, CL; Fernandez, JM; Li, L | 1 |
| Carson, M; Chattopadhyay, D; Delucas, L; Howard, A; Pal, B; Schormann, N; Senkovich, O; Smith, C | 1 |
| Ainavarapu, SR; Fernandez, JM; Li, L | 1 |
| Ahmad, MF; Bennett, BC; Dealwis, CG; Langan, P; Wan, Q | 1 |
| Fairlamb, AH; Frearson, JA; Ong, HB; Robinson, DA; Shanks, EJ; Sienkiewicz, N; Thompson, S | 1 |
| Lee, AL; Mauldin, RV | 1 |
| Abali, EE; Banerjee, D; Bertino, JR; Felczak, K; Gounder, M; Johnson-Farley, N; Kerrigan, JE; Lin, H; Pankiewicz, KW; Roussel, B; Scotto, KW | 1 |
| Abali, EE; Adebisi Lawal, R; Banerjee, D; Bertino, JR; Hsieh, YC; Johnson-Farley, N; Kerrigan, JE; Lee, KC; Scotto, K; Tedeschi, P | 1 |
| Bertino, JR; Boros, LG; Chan, LL; DiPaola, RS; Dolfi, SC; Dvorzhinski, D; Gounder, M; Hirshfield, KM; Lin, H; Markert, EK; Oltvai, ZN; Qiu, J; Tedeschi, PM; Vazquez, A | 1 |
| Brodbelt, JS; Cammarata, MB; Ellington, A; Rosenberg, J; Thyer, R | 1 |
| Agathocleous, M; DeBerardinis, RJ; Hu, Z; Huddlestun, SE; Johnson, TM; Leitch, AM; Morrison, SJ; Murphy, MM; Piskounova, E; Zhao, Z | 1 |
| Akashi, S; Arai, S; Hamachi, I; Ikegami, T; Konuma, T; Saikusa, K; Sakamoto, S; Takano, K; Ushijima, H | 1 |
| Gupta, R; Kaiser, CM; Toptygin, D | 1 |
| Chen, C; Chen, W; Dan, S; Huang, D; Jiang, Y; Lai, X; Liu, L; Xie, L; Yang, Y; Yu, Z; Zhang, Y; Zhao, Y; Zheng, J | 1 |
Reviews
3 review(s) available for methotrexate and nadp
| Article | Year |
|---|---|
Folic acid and vitamin B12: transport and conversion to coenzyme forms.
Topics: Animals; Biological Transport, Active; Cell Line; Cell Membrane; Coenzymes; Folic Acid; Kinetics; Lacticaseibacillus casei; Leukemia L1210; Methotrexate; Models, Biological; Mutation; NADP; Tetrahydrofolate Dehydrogenase; Tetrahydrofolates; Vitamin B 12 | 1974 |
Inhibition of folate biosynthesis and function as a basis for chemotherapy.
Topics: Aminobenzoates; Animals; Binding Sites; Biological Transport; Drug Resistance, Microbial; Drug Synergism; Escherichia coli; Folic Acid; Folic Acid Antagonists; Glutamates; Guinea Pigs; Humans; Lactobacillus; Leucovorin; Methotrexate; NAD; NADP; Pteridines; Pyrimidines; Rabbits; Rats; Sulfonamides | 1965 |
Methotrexate. I. Pharmacology and pharmacokinetics.
Topics: Aminopterin; Chromatography, High Pressure Liquid; Humans; Hydrogen-Ion Concentration; Kinetics; Methotrexate; NADP; Protein Binding; Radioimmunoassay; Tetrahydrofolate Dehydrogenase; Urine | 1984 |
Other Studies
160 other study(ies) available for methotrexate and nadp
| Article | Year |
|---|---|
Methotrexate resistance of mouse dihydrofolate reductase: effect of substitution of phenylalanine-31 by serine or tryptophan.
Topics: Animals; Crystallography, X-Ray; Folic Acid Antagonists; Kinetics; Methotrexate; Mice; Mutagenesis, Site-Directed; Phenylalanine; Serine; Tetrahydrofolate Dehydrogenase; Thermodynamics; Tryptophan | 1996 |
Substrate and inhibitor complexes of dihydrofolate reductase from amethopterin-resistant L1210 cells.
Topics: Animals; Binding Sites; Cells, Cultured; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Leukemia L1210; Methotrexate; NADP; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase | 1975 |
Intrinsic resistance to methotrexate of cultured mammalian cells in relation to the inhibition kinetics of their dihydrololate reductases.
Topics: Biological Transport; Cell Membrane; Cells, Cultured; Drug Resistance; Folic Acid; Folic Acid Antagonists; Kinetics; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase; Thymidylate Synthase | 1976 |
Interaction of 1,N6-ethenoadenine derivatives of triphosphopyridine and reduced triphosphopyridine nucleotides with dihydrofolate reductase from amethopterin-resistant L1210 cells.
Topics: Animals; Binding Sites; Drug Resistance; Kinetics; Leukemia L1210; Methotrexate; Mice; NADP; Oxidation-Reduction; Protein Binding; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase | 1976 |
Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei.
Topics: Amino Acids; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Drug Resistance, Microbial; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Isoelectric Focusing; Kinetics; Lacticaseibacillus casei; Methotrexate; Molecular Weight; NADP; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase | 1976 |
Two distinct types of trimethoprim-resistant dihydrofolate reductase specified by R-plasmids of different compatibility groups.
Topics: Chromosomes, Bacterial; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Methotrexate; NADP; Phenotype; Plasmids; Protein Biosynthesis; Species Specificity; Tetrahydrofolate Dehydrogenase; Transcription, Genetic; Trimethoprim | 1977 |
A 13C nuclear magnetic resonance study of the interaction of ligands with arginine residues in dihydrofolate reductase.
Topics: Arginine; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Protein Binding; Streptococcus; Tetrahydrofolate Dehydrogenase; Urea | 1977 |
Dihydrofolate reductase: x-ray structure of the binary complex with methotrexate.
Topics: Binding Sites; Escherichia coli; Folic Acid Antagonists; Methotrexate; Molecular Conformation; NADP; Protein Conformation; Tetrahydrofolate Dehydrogenase; X-Ray Diffraction | 1977 |
Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies.
Topics: Animals; Chromatography, Affinity; Circular Dichroism; Drug Resistance; Leukemia L1210; Methotrexate; Molecular Weight; NADP; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase; Tryptophan | 1977 |
Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. Effects of pH, salts, temperature, and source of NADPH on enzyme activity and substrate specificity studies.
Topics: Buffers; Drug Resistance, Microbial; Enzyme Activation; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Lacticaseibacillus casei; Methotrexate; Molecular Weight; NADP; Osmolar Concentration; Protein Conformation; Salts; Temperature; Tetrahydrofolate Dehydrogenase | 1977 |
Dihydrofolate reductase from soybean seedlings: a fluorescence and circular dichroism study.
Topics: Circular Dichroism; Folic Acid; Glycine max; Guanidines; Methotrexate; NADP; Plants; Protein Binding; Protein Conformation; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase | 1977 |
Effect of temperature on fluorescence and circular dichroism of Escherichia coli dihydrofolate reductase and its complexes.
Topics: Circular Dichroism; Escherichia coli; Ligands; Methotrexate; NADP; Protein Conformation; Pyrimethamine; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Temperature; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1978 |
Nuclear magnetic resonance studies on bacterial dihydrofolate reductase containing [methyl-13C]methionine.
Topics: Binding Sites; Carbon Isotopes; Chemical Phenomena; Chemistry; Magnetic Resonance Spectroscopy; Methionine; Methotrexate; NADP; Nucleotides; Pteridines; Streptococcus; Temperature; Tetrahydrofolate Dehydrogenase | 1978 |
Interaction of methotrexate, folates, and pyridine nucleotides with dihydrofolate reductase: calorimetric and spectroscopic binding studies.
Topics: Animals; Calorimetry; Chickens; Folic Acid; Liver; Methotrexate; NAD; NADP; Protein Binding; Protons; Spectrum Analysis; Tetrahydrofolate Dehydrogenase; Thermodynamics | 1978 |
Dihydrofolate reductase from Lactobacillus casei. X-ray structure of the enzyme methotrexate.NADPH complex.
Topics: Amino Acid Sequence; Lacticaseibacillus casei; Methotrexate; Models, Molecular; NADP; Oxidation-Reduction; Protein Binding; Protein Conformation; Tetrahydrofolate Dehydrogenase; X-Ray Diffraction | 1978 |
Interpretation of nuclear magnetic resonance spectra for Lactobacillus casei dihydrofolate reductase based on the X-ray structure of the enzyme-methotrexate-NADPH complex.
Topics: Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Methotrexate; Models, Molecular; NADP; Protein Binding; Protein Conformation; Tetrahydrofolate Dehydrogenase; Tryptophan; X-Ray Diffraction | 1979 |
Circular-dichroism studies of ligand binding to dihydrofolate reductase from Lactobacillus casei MTX/R.
Topics: Circular Dichroism; Folic Acid; Lacticaseibacillus casei; Ligands; Methotrexate; NADP; Protein Binding; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1979 |
Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase.
Topics: Kinetics; Mathematics; Methotrexate; NADP; Oxidation-Reduction; Protein Binding; Streptococcus; Tetrahydrofolate Dehydrogenase | 1979 |
Effect of N-bromosuccinimide modification on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli. Activity, spectrophotometric, fluorescence and circular dichroism studies.
Topics: Amino Acids; Binding Sites; Circular Dichroism; Drug Resistance, Microbial; Escherichia coli; Hydrogen-Ion Concentration; Kinetics; Methotrexate; NADP; Oxidation-Reduction; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Succinimides; Sulfhydryl Compounds; Tetrahydrofolate Dehydrogenase; Tryptophan | 1975 |
A nuclear magnetic resonance study of nicotinamide adenine dinucleotide phosphate binding to Lactobacillus casei dihydrofolate reductase.
Topics: Amides; Aminopterin; Binding Sites; Carbon Isotopes; Carboxylic Acids; Folic Acid; Kinetics; Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Mathematics; Methotrexate; NADP; Protein Binding; Protein Conformation; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1975 |
Circular dichroism studies of dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B, MB 1428: ternary complexes.
Topics: Adenosine Diphosphate Sugars; Binding Sites; Circular Dichroism; Drug Resistance, Microbial; Escherichia coli; Folic Acid; Methotrexate; NAD; NADP; Nicotinamide Mononucleotide; Protein Binding; Protein Conformation; Ribose; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase | 1975 |
Crystal structure determination at 2.3 A of recombinant human dihydrofolate reductase ternary complex with NADPH and methotrexate-gamma-tetrazole.
Topics: Crystallography; Drug Interactions; Humans; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase; Tetrazoles | 1992 |
13C NMR studies of complexes of Escherichia coli dihydrofolate reductase formed with methotrexate and with folic acid.
Topics: Escherichia coli; Folic Acid; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase | 1992 |
31P-NMR assignment and conformational study of NADPH bound to Lactobacillus casei dihydrofolate reductase based on two-dimensional 1H-31P-heteronuclear and 1H-detected 1H-31P-shift-correlation experiments.
Topics: Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Methotrexate; Molecular Conformation; NADP; Phosphates; Tetrahydrofolate Dehydrogenase | 1992 |
Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution.
Topics: Amino Acid Sequence; Hydrogen Bonding; Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Methotrexate; Molecular Sequence Data; NADP; Nitrogen Isotopes; Protein Conformation; Solutions; Tetrahydrofolate Dehydrogenase; X-Ray Diffraction | 1991 |
Investigation of the functional role of tryptophan-22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis.
Topics: Coenzymes; Deuterium; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Mutagenesis, Site-Directed; NADP; Protein Binding; Structure-Activity Relationship; Substrate Specificity; Tetrahydrofolate Dehydrogenase; Tryptophan | 1991 |
The importance of loop region residues 40-46 in human dihydrofolate reductase as revealed by site-directed mutagenesis.
Topics: Antibodies; Base Sequence; Binding, Competitive; Cloning, Molecular; Cyanogen Bromide; Enzyme Activation; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Folic Acid; Humans; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Molecular Sequence Data; Mutation; NADP; Peptide Fragments; Protein Conformation; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Transformation, Bacterial | 1990 |
Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate.
Topics: Binding Sites; Folic Acid; Folic Acid Antagonists; Humans; Methotrexate; Models, Molecular; Molecular Structure; NADP; Protein Conformation; Tetrahydrofolate Dehydrogenase; X-Ray Diffraction | 1990 |
Dihydrofolate reductase: multiple conformations and alternative modes of substrate binding.
Topics: Escherichia coli; Hydrogen-Ion Concentration; Lacticaseibacillus casei; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; Models, Molecular; NADH, NADPH Oxidoreductases; NADP; Niacinamide; Protein Conformation; Substrate Specificity; Tetrahydrofolate Dehydrogenase | 1989 |
Automated site-directed drug design: the prediction and observation of ligand point positions at hydrogen-bonding regions on protein surfaces.
Topics: Drug Design; Hydrogen Bonding; Information Systems; Ligands; Methotrexate; NADP; Proteins; Software; Tetrahydrofolate Dehydrogenase; Thermodynamics; Trypsin | 1989 |
A fluorescence study of substrate and inhibitor binding to bovine liver dihydrofolate reductase.
Topics: Animals; Binding, Competitive; Cattle; Folic Acid; Folic Acid Antagonists; Kinetics; Liver; Methotrexate; NADP; Protein Binding; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase | 1989 |
Effects of conversion of phenylalanine-31 to leucine on the function of human dihydrofolate reductase.
Topics: Binding Sites; Folic Acid; Humans; Kinetics; Leucine; Methotrexate; Mutation; NADP; Phenylalanine; Recombinant Proteins; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Trimethoprim; X-Ray Diffraction | 1989 |
Activity of antifolates against Pneumocystis carinii dihydrofolate reductase and identification of a potent new agent.
Topics: Biological Transport; Folic Acid Antagonists; Kinetics; Methotrexate; NADP; Pneumocystis; Quinazolines; Tetrahydrofolate Dehydrogenase; Trimetrexate | 1987 |
Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.
Topics: Amino Acid Sequence; Animals; Binding Sites; DNA; DNA, Recombinant; Drug Resistance; Drug Stability; Escherichia coli; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Mice; Molecular Sequence Data; Mutation; NADP; Protein Conformation; Recombinant Proteins; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1988 |
Methotrexate analogues. 30. Dihydrofolate reductase inhibition and in vitro tumor cell growth inhibition by N epsilon-(haloacetyl)-L-lysine and N delta-(haloacetyl)-L-ornithine analogues and an acivicin analogue of methotrexate.
Topics: Acetates; Animals; Candida albicans; Chemical Phenomena; Chemistry; Folic Acid Antagonists; Isoxazoles; Leukemia L1210; Lysine; Methotrexate; Mice; NADP; Ornithine; Protein Conformation; Spectrophotometry | 1987 |
Dihydrofolate reductase: specificity and conformational change.
Topics: Binding Sites; Folic Acid; Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Protein Conformation; Substrate Specificity; Tetrahydrofolate Dehydrogenase | 1987 |
Thermodynamic study of the influence of NADPH on the binding of methotrexate and its metabolites to a mammalian dihydrofolate reductase.
Topics: Animals; Calorimetry; Cattle; Kinetics; Liver; Mathematics; Methotrexate; NADP; Oxidation-Reduction; Potentiometry; Protein Binding; Tetrahydrofolate Dehydrogenase | 1988 |
Mechanism of inhibition of dihydrofolate reductases from bacterial and vertebrate sources by various classes of folate analogues.
Topics: Animals; Binding, Competitive; Chickens; Escherichia coli; Folic Acid; Folic Acid Antagonists; Leucovorin; Liver; Methotrexate; NADP; Pteridines; Pyrimethamine; Tetrahydrofolate Dehydrogenase; Triazines; Trimethoprim | 1986 |
Kinetic and molecular properties of the dihydrofolate reductase from pyrimethamine-sensitive and pyrimethamine-resistant clones of the human malaria parasite Plasmodium falciparum.
Topics: Chromatography, Affinity; Drug Resistance; Folic Acid Antagonists; Humans; Kinetics; Malaria; Methotrexate; NADP; Plasmodium falciparum; Pyrimethamine; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1987 |
Electrostatic interaction energy and solvent accessibility in the methotrexate-reduced nicotinamide adenine dinucleotide phosphate-dihydrofolate reductase ternary complex.
Topics: Electrochemistry; Methotrexate; NADP; Protein Conformation; Tetrahydrofolate Dehydrogenase | 1987 |
pH-Stat titration method for dihydrofolate reductase activity measurements: application to determination of substrate Michaelis constant and antifolate inhibition constant.
Topics: Animals; Cattle; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Liver; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase | 1986 |
A re-evaluation of the competitive protein binding assay for methotrexate binding to dihydrofolate reductase.
Topics: Adsorption; Binding, Competitive; Charcoal; Humans; In Vitro Techniques; Methotrexate; Molecular Weight; NADP; Tetrahydrofolate Dehydrogenase | 1986 |
Lack of dihydrofolate reductase in human tumor and leukemia cells in vivo.
Topics: Animals; Cattle; Cell Line; Cells, Cultured; Chickens; Humans; Leukemia, Experimental; Liver; Methotrexate; NADP; Neoplasms; Tetrahydrofolate Dehydrogenase; Tissue Distribution | 1985 |
Turnover of dihydrofolate reductase in rapidly dividing cells.
Topics: Animals; Carbon Isotopes; Cell Division; Cells; Chromatography, Affinity; Drug Resistance, Microbial; Half-Life; Isotope Labeling; Kinetics; Lacticaseibacillus casei; Leucine; Leukemia L1210; Methotrexate; Mice; NADP; Polysaccharides; Tetrahydrofolate Dehydrogenase; Time Factors | 1973 |
Circular dichroism studies of dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
Topics: Binding Sites; Chromatography, Ion Exchange; Circular Dichroism; Drug Resistance, Microbial; Escherichia coli; Folic Acid; Mathematics; Methotrexate; NADP; Optical Rotatory Dispersion; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase; Ultraviolet Rays | 1972 |
Two different effects of mutation on the binding of folate antagonists by a microbial dihydrofolate reductase.
Topics: Amines; Amino Acids; Aminopterin; Binding Sites; Cell-Free System; Folic Acid; Folic Acid Antagonists; Genes; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Mutation; NADP; Pteridines; Pyrimethamine; Pyrimidines; Species Specificity; Streptococcus pneumoniae; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1973 |
Methotrexate binding to dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
Topics: Binding Sites; Chromatography, Affinity; Chromatography, Gel; Chromatography, Ion Exchange; Drug Resistance, Microbial; Escherichia coli; Folic Acid; Folic Acid Antagonists; Kinetics; Methotrexate; NADP; Osmolar Concentration; Oxidation-Reduction; Protein Binding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase; Time Factors | 1973 |
Evidence for two reduced triphosphopyridine nucleotide binding sites on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
Topics: Binding Sites; Chromatography, Affinity; Drug Resistance, Microbial; Escherichia coli; Kinetics; Mathematics; Methotrexate; NADP; Osmolar Concentration; Protein Binding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase; Time Factors; Ultrafiltration | 1973 |
Glucose-6-phosphate dehydrogenase in the blood of psoriatics, and the effects of methotrexate.
Topics: Dermatitis, Atopic; Enzyme Repression; Female; Glucosephosphate Dehydrogenase; Humans; Male; Methotrexate; Mycosis Fungoides; NAD; NADP; Psoriasis; Spectrophotometry, Ultraviolet; Time Factors | 1973 |
Dihydrofolate reductase from a methotrexate-resistant Escherichia coli.
Topics: Binding Sites; Drug Resistance, Microbial; Escherichia coli; Hydrogen-Ion Concentration; Methotrexate; NAD; NADP; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Ultrafiltration | 1974 |
Implication of a tryptophyl residue in the active site of dihydrofolate reductase.
Topics: Amino Acids; Binding Sites; Chromatography, Gel; Chromatography, Ion Exchange; Dialysis; Drug Resistance, Microbial; Electrophoresis, Polyacrylamide Gel; Folic Acid Antagonists; Lacticaseibacillus casei; Methotrexate; NADP; Oxidation-Reduction; Protein Denaturation; Spectrophotometry, Ultraviolet; Succinimides; Tetrahydrofolate Dehydrogenase; Tryptophan; Urea | 1974 |
The effect of histidine modification on the activity of dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B.
Topics: Allosteric Regulation; Allosteric Site; Amino Acids; Anhydrides; Binding Sites; Circular Dichroism; Escherichia coli; Ethyl Ethers; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamines; Kinetics; Ligands; Methotrexate; Mutation; NADP; Oxidation-Reduction; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase; Time Factors | 1974 |
Conformational changes induced in dihydrofolate reductase by folates, pyridine nucleotide coenzymes, and methotrexate.
Topics: Folic Acid; Lacticaseibacillus casei; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Protein Conformation; Protein Denaturation; Tetrahydrofolate Dehydrogenase; Urea | 1974 |
The estimation of enzyme activity in living epidermal cells.
Topics: Animals; Aspartate Aminotransferases; Chemistry Techniques, Analytical; Cytoplasm; Epithelial Cells; Epithelium; Forearm; Glycolysis; Guinea Pigs; Humans; In Vitro Techniques; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Liver; Malate Dehydrogenase; Malates; Methotrexate; Mitochondria; NAD; NADP; Ouabain; Oxygen; Prednisolone; Psoriasis; Pyruvate Kinase; Skin; Spectrophotometry | 1974 |
Folic acid metabolism in vitamin B12-deficient sheep. Effects of injected methionine on methotrexate transport and the activity of enzymes associated with folate metabolism in liver.
Topics: Animals; Biological Transport; Carbon Radioisotopes; Female; Folic Acid; Homocysteine; Liver; Membranes; Methionine; Methotrexate; Methyltransferases; NAD; NADP; S-Adenosylmethionine; Serum Albumin, Bovine; Sheep; Tetrahydrofolate Dehydrogenase; Tritium; Vitamin B 12; Vitamin B 12 Deficiency | 1974 |
Inhibitor binding analysis of dihydrofolate reductases from various species.
Topics: Amino Acids; Animals; Catalysis; Enzymes; Escherichia coli; Guinea Pigs; Humans; In Vitro Techniques; Kinetics; Liver; Methotrexate; NADP; Proteins; Pyrimidines; Rabbits; Rats; Species Specificity; Staphylococcus; Tetrahydrofolate Dehydrogenase; Triazines | 1965 |
Differentiating properties of the dihydrofolate reductases of amethopterin-resistant Streptococcus faecalis/Ak and the sensitive parent strain.
Topics: Chromatography, Gel; Drug Resistance, Microbial; Enterococcus faecalis; In Vitro Techniques; Kinetics; Methotrexate; Mutation; NADP; Tetrahydrofolate Dehydrogenase | 1966 |
Dihydrofolate reductase from the L1210R murine lymphoma. Fluorometric measurements of the interaction of the enzyme with coenzymes, substrates, and inhibitors.
Topics: Animals; Chemical Phenomena; Chemistry; Enzymes; Fluorometry; Folic Acid; Leukemia L1210; Lymphoma; Methotrexate; Mice; NADP; Triamterene | 1966 |
An isotopic assay for dihydrofolate reductase.
Topics: Animals; Charcoal; In Vitro Techniques; Liver; Methotrexate; NADP; Nitrites; Rats; Tetrahydrofolate Dehydrogenase; Tritium | 1966 |
Dihydrofolate reductase from Trypanosoma equiperdum. I. Isolation, partial purification, and properties.
Topics: Aminopterin; Animals; Folic Acid; Folic Acid Antagonists; Hot Temperature; Hydrogen-Ion Concentration; In Vitro Techniques; Male; Methotrexate; NAD; NADP; Pyrimethamine; Rats; Tetrahydrofolate Dehydrogenase; Trypanosoma | 1967 |
Dihydrofolate reductase from the L1210 R murine lymphoma. Further studies on the binding of substrates and inhibitors to the enzyme.
Topics: Animals; Binding Sites; Chemical Phenomena; Chemistry, Physical; Fluorometry; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Leukemia L1210; Methotrexate; Mice; NADP; Protein Binding; Pyrimidines; Tetrahydrofolate Dehydrogenase; Triamterene | 1967 |
Dihydrofolic reductase in human blood cells. I. Techniques for the study of enzyme activity and intracellular amethopterin content.
Topics: Animals; Binding Sites; Chickens; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Liver; Methotrexate; NADP; Spectrophotometry; Tetrahydrofolate Dehydrogenase | 1967 |
The essential SH-groups in folate reductase.
Topics: Animals; Benzoates; Binding Sites; Chemical Phenomena; Chemistry; Culture Techniques; Folic Acid; Folic Acid Antagonists; Methotrexate; NADP; Sarcoma 180; Sulfhydryl Compounds; Temperature; Tetrahydrofolate Dehydrogenase; Urea | 1968 |
Methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A and its repressibility by serine.
Topics: Aminohydrolases; Culture Media; Enzyme Repression; Kinetics; Ligases; Methotrexate; NADP; Protein Hydrolysates; Serine; Spectrophotometry; Streptococcus; Tetrahydrofolate Dehydrogenase; Transferases | 1968 |
The presence of a substance with hydrogen transporting properties in some samples of methotrexate.
Topics: Benzoates; Chemical Phenomena; Chemistry; Chromatography, Paper; Colorimetry; Drug Contamination; Drug Storage; Freeze Drying; Hydrogen; Methotrexate; Mitosis; NAD; NADP; Oxidation-Reduction; Tetrazolium Salts | 1968 |
Presence and properties of dihydrofolate reductases within the genus Crithidia.
Topics: Animals; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Methotrexate; NADP; Pteridines; Pyrimethamine; Pyrimidines; Tetrahydrofolate Dehydrogenase; Trypanosoma | 1969 |
Folate reductase of the amethopterin-resistant Streptococcus faecium var. durans-Ak. I. Inhibition by amethopterin and methasquin, a new quinazoline antifolate.
Topics: Aspartic Acid; Chromatography, DEAE-Cellulose; Electrophoresis; Folic Acid Antagonists; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Molecular Weight; NADP; Potassium Chloride; Quinazolines; Streptococcus; Tetrahydrofolate Dehydrogenase | 1970 |
Bacteriophage tail components. II. Dihydrofolate reductase in T4D bacteriophage.
Topics: Aminopterin; Animals; Binding Sites; Coliphages; Escherichia coli; Fluorometry; Folic Acid Antagonists; Formamides; Genes; Genetic Complementation Test; Genetics, Microbial; Immune Sera; Methotrexate; Mutation; NAD; NADP; Rabbits; Solvents; Spectrophotometry; Tetrahydrofolate Dehydrogenase; Urea; Viral Proteins; Virus Replication | 1970 |
"Induction" of dihydrofolate reductase: purification and properties of the "induced" human erythrocyte and leukocyte enzyme and normal bone marrow enzyme.
Topics: Animals; Bone Marrow; Bone Marrow Cells; Chromatography; Enzyme Activation; Enzyme Induction; Erythrocytes; Folic Acid; Humans; Hydrogen-Ion Concentration; Immune Sera; Leukemia L1210; Leukocytes; Methotrexate; Molecular Weight; NAD; NADP; Neoplasms; Protein Denaturation; Tetrahydrofolate Dehydrogenase | 1970 |
A soluble dihydrofolate reductase from human placenta: purification and properties.
Topics: Chemical Precipitation; Chromatography, Gel; Chromatography, Ion Exchange; Enzyme Activation; Female; Folic Acid Antagonists; Humans; Hydrogen-Ion Concentration; Hydroxyapatites; Kinetics; Methotrexate; Molecular Weight; NADP; Placenta; Potassium Chloride; Solubility; Tetrahydrofolate Dehydrogenase; Urea | 1971 |
Interconversion of the multiple forms of dihydrofolate reductase from amethopterin-resistant Lactobacillus casei.
Topics: Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Chromatography, Thin Layer; Drug Resistance, Microbial; Electrophoresis; Fluorescence; Folic Acid; Glutathione Reductase; Hot Temperature; Hydrogen-Ion Concentration; Lactobacillus; Methotrexate; Molecular Weight; NADP; Protein Denaturation; Spectrophotometry; Tetrahydrofolate Dehydrogenase | 1971 |
Affinity chromatography of dihydrofolate reductase.
Topics: Chromatography; Cyanogen Bromide; Electrophoresis, Disc; Folic Acid; Hydrogen-Ion Concentration; Lactobacillus; Methods; Methotrexate; NADP; Polysaccharides; Tetrahydrofolate Dehydrogenase | 1971 |
Proton magnetic resonance studies of folate and folate antagonists.
Topics: Aminopterin; Chemical Phenomena; Chemistry; Deuterium; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Structure-Activity Relationship; Temperature | 1971 |
Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei.
Topics: Acetates; Amino Acids; Ammonium Sulfate; Chromatography; Chromatography, Gel; Chromatography, Ion Exchange; Drug Resistance, Microbial; Electrophoresis; Folic Acid; Folic Acid Antagonists; Hydroxyapatites; Lacticaseibacillus casei; Lactobacillus; Methotrexate; Molecular Weight; NADP; Protein Binding; Sodium Dodecyl Sulfate; Spectrophotometry; Tetrahydrofolate Dehydrogenase; Ultracentrifugation; Ultraviolet Rays; Urea | 1972 |
Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli.
Topics: Amino Acids; Chromatography, Affinity; Chromatography, Gel; Cyanogen Bromide; Dansyl Compounds; Drug Resistance, Microbial; Electrophoresis; Electrophoresis, Disc; Escherichia coli; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Methotrexate; NAD; NADP; Osmolar Concentration; Potassium Chloride; Sodium Chloride; Tetrahydrofolate Dehydrogenase; Ultracentrifugation | 1972 |
Dihydrofolate reductase activity in strains of Streptococcus faecium var. durans resistant to methasquin and amethopterin.
Topics: Aspartic Acid; Benzoates; Chromatography, Gel; Drug Resistance, Microbial; Folic Acid Antagonists; Genetics, Microbial; Methotrexate; Mutation; NADP; Quinolines; Spectrophotometry; Streptococcus; Tetrahydrofolate Dehydrogenase | 1972 |
Purification of tetrahydrofolate dehydrogenase by affinity chromatography.
Topics: Alkylation; Ammonium Sulfate; Animals; Buffers; Cell Line; Chemical Precipitation; Chromatography, Affinity; Chromatography, Gel; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Leukemia L1210; Methods; Methotrexate; NADP; Osmolar Concentration; Polysaccharides; Protein Binding; Spectrophotometry; Tetrahydrofolate Dehydrogenase; Ultraviolet Rays | 1972 |
Two different species of dihydrofolate reductase in mammalian cells differentially resistant to amethopterin and methasquin.
Topics: Animals; Cell Line; Cricetinae; Drug Resistance; Folic Acid Antagonists; Hydrogen-Ion Concentration; In Vitro Techniques; Isoenzymes; Kinetics; Methotrexate; Molecular Weight; Mutagens; NADP; Quinazolines; Tetrahydrofolate Dehydrogenase | 1972 |
Dihydrofolate reductase from mouse liver and spleen. Purification, properties and inhibition by substituted 2,4-diaminopyrimidines and 4,6-diaminotriazines.
Topics: Animals; Antigen-Antibody Reactions; Cell Line; Chromatography; Chromatography, Gel; Enzyme Activation; Folic Acid; Folic Acid Antagonists; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Leukemia L1210; Liver; Lymphoma; Methotrexate; Mice; Mice, Inbred Strains; NADP; Potassium Chloride; Protein Denaturation; Pyrimidines; Spleen; Tetrahydrofolate Dehydrogenase; Triazines; Urea | 1971 |
Circular dichroic studies on the interaction of dihydrofolate reductase with substrates, coenzymes, and inhibitors.
Topics: Circular Dichroism; Folic Acid; Folic Acid Antagonists; Hydrogen-Ion Concentration; Methotrexate; NADP; Oxidation-Reduction; Protein Binding; Streptococcus; Tetrahydrofolate Dehydrogenase; Ultraviolet Rays | 1972 |
TPNH-dependent binding of amethopterin by dihydrofolate reductase from Lactobacillus casei.
Topics: Ammonium Sulfate; Crystallization; Electrophoresis, Polyacrylamide Gel; Folic Acid Antagonists; Lacticaseibacillus casei; Lactobacillus; Methotrexate; NADP; Protein Binding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase | 1972 |
Dihydrofolate reductases of Escherichia coli and bacteriophage Tr. A spectrofluorometric study.
Topics: Amino Acids; Binding Sites; Chlorides; Chromatography, Affinity; Coliphages; Electrophoresis, Disc; Escherichia coli; Fluorides; Folic Acid; Folic Acid Antagonists; Kinetics; Methotrexate; NADP; Osmolar Concentration; Peptides; Perchlorates; Sodium Dodecyl Sulfate; Species Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Streptomycin; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Thiocyanates | 1973 |
Circular dichroism studies in the near UV of ligand binding to chicken liver dihydrofolate reductase.
Topics: Animals; Chickens; Circular Dichroism; Folic Acid; Liver; Methotrexate; NADP; Spectrophotometry, Ultraviolet; Tetrahydrofolate Dehydrogenase | 1983 |
Immunochemical evidence for extensive ligand-induced conformational changes in Lactobacillus casei dihydrofolate reductase.
Topics: Animals; Antibody Specificity; Antigen-Antibody Complex; Female; Lacticaseibacillus casei; Methotrexate; NADP; Protein Conformation; Rabbits; Radioimmunoassay; Tetrahydrofolate Dehydrogenase | 1984 |
19F-n.m.r. studies of 3',5'-difluoromethotrexate binding to Lactobacillus casei dihydrofolate reductase. Molecular motion and coenzyme-induced conformational changes.
Topics: Folic Acid Antagonists; Lacticaseibacillus casei; Macromolecular Substances; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Protein Conformation; Tetrahydrofolate Dehydrogenase | 1984 |
Role of the cellular oxidation-reduction state in methotrexate binding to dihydrofolate reductase and dissociation induced by reduced folates.
Topics: Animals; Azides; Carcinoma, Ehrlich Tumor; Folic Acid Antagonists; Glucose; Indicators and Reagents; Kinetics; Leucovorin; Methotrexate; Mice; NAD; NADP; Oxidation-Reduction; Potassium Cyanide; Protein Binding; Rotenone; Sodium Azide; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase | 1984 |
Kinetics of ternary complex formation between dihydrofolate reductase, coenzyme, and inhibitors.
Topics: Hydrogen-Ion Concentration; Kinetics; Lacticaseibacillus casei; Methotrexate; NADP; Protein Binding; Pyrimethamine; Pyrimidines; Spectrometry, Fluorescence; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1980 |
Photo-CIDNP studies of the influence of ligand binding on the surface accessibility of aromatic residues in dihydrofolate reductase.
Topics: Folic Acid; Histidine; Lacticaseibacillus casei; Lasers; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Photochemistry; Protein Binding; Protein Conformation; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1980 |
Interaction of the carboxamide of NADPH, with Lactobacillus casei dihydrofolate reductase.
Topics: Lacticaseibacillus casei; Methotrexate; NADP; Spectrum Analysis, Raman; Tetrahydrofolate Dehydrogenase | 1982 |
A mechanism of resistance to methotrexate. NADPH but not NADH stimulation of methotrexate binding to dihydrofolate reductase.
Topics: Animals; Drug Resistance; Kinetics; Leukemia L1210; Methotrexate; Mice; NAD; NADP; Protein Binding; Tetrahydrofolate Dehydrogenase | 1983 |
Inhibition of dihydrofolate reductase by methotrexate: a new look at an old problem.
Topics: Animals; Catalysis; Cells, Cultured; Cysteine; Folic Acid Antagonists; Histidine; Kinetics; Leukemia L1210; Methotrexate; Mice; NADP; Structure-Activity Relationship | 1980 |
Kinetics of substrate, coenzyme, and inhibitor binding to Escherichia coli dihydrofolate reductase.
Topics: Escherichia coli; Fluorescence; Folic Acid; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1981 |
Dihydrofolate reductase hysteresis and its effect of inhibitor binding analyses.
Topics: Binding Sites; Escherichia coli; Isoenzymes; Kinetics; Methotrexate; NADP; Oxidation-Reduction; Protein Binding; Sulfamethoxazole; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1981 |
Activity of sulfa drugs and dihydrofolate reductase inhibitors against Candida albicans.
Topics: Aminopterin; Animals; Candida albicans; Cattle; Folic Acid Antagonists; Kinetics; Liver; Methotrexate; NADP; Trimethoprim | 1982 |
Nuclear magnetic resonance study of interaction of ligands with streptococcus faecium dihydrofolate reductase labeled with [gamma-13C]tryptophan.
Topics: Adenosine Diphosphate Ribose; Carbon Isotopes; Folic Acid; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Pteroylpolyglutamic Acids; Pyrimidines; Spin Labels; Streptococcus; Tetrahydrofolate Dehydrogenase; Tryptophan | 1982 |
Improved enzymatic assay for methotrexate: shape of standard curve, stability of reagents, sensitivity.
Topics: Drug Stability; Humans; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase | 1981 |
Enzymic inhibition assay for methotrexate with a discrete analyzer, the ABA-100.
Topics: Albumins; Animals; Cattle; Folic Acid Antagonists; Humans; Liver; Methotrexate; NADP; Radioimmunoassay; Tetrahydrofolate Dehydrogenase | 1980 |
A simplified radioenzymatic assay for dihydrofolate reductase using [3H]dihydrofolate.
Topics: Dithionite; Folic Acid; Fractional Precipitation; Humans; Kinetics; Methods; Methotrexate; NADP; Oxidation-Reduction; Sulfates; Tetrahydrofolate Dehydrogenase; Tritium; Zinc; Zinc Sulfate | 1980 |
Telluromethionine in structural biochemistry.
Topics: Crystallization; Crystallography, X-Ray; Escherichia coli; Magnetic Resonance Spectroscopy; Methionine; Methotrexate; NADP; Tellurium; Tetrahydrofolate Dehydrogenase | 1995 |
Thermal denaturation of bacterial and bovine dihydrofolate reductases and their complexes with NADPH, trimethoprim and methotrexate.
Topics: Animals; Calorimetry, Differential Scanning; Cattle; Escherichia coli; Hot Temperature; Isoelectric Point; Liver; Methotrexate; NADP; Osmolar Concentration; Protein Denaturation; Tetrahydrofolate Dehydrogenase; Trimethoprim | 1995 |
Detection of long-lived bound water molecules in complexes of human dihydrofolate reductase with methotrexate and NADPH.
Topics: Amino Acid Sequence; Binding Sites; Humans; Magnetic Resonance Spectroscopy; Methotrexate; Models, Molecular; Molecular Conformation; Molecular Sequence Data; NADP; Protons; Recombinant Proteins; Tetrahydrofolate Dehydrogenase; Water | 1995 |
Contributions of tryptophan 24 and glutamate 30 to binding long-lived water molecules in the ternary complex of human dihydrofolate reductase with methotrexate and NADPH studied by site-directed mutagenesis and nuclear magnetic resonance spectroscopy.
Topics: Amino Acid Sequence; Glutamic Acid; Humans; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Methotrexate; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Recombinant Proteins; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Tryptophan; Water | 1995 |
Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.
Topics: Amino Acid Sequence; Binding Sites; Crystallography, X-Ray; Drug Resistance; Enzyme Stability; Genetic Variation; Humans; Hydrogen-Ion Concentration; Kinetics; Leucine; Methotrexate; Models, Molecular; Mutagenesis, Site-Directed; NADP; Point Mutation; Protein Conformation; Recombinant Proteins; Tetrahydrofolate Dehydrogenase | 1995 |
Nonadditivity of mutational effects at the folate binding site of Escherichia coli dihydrofolate reductase.
Topics: Base Sequence; Binding Sites; Binding, Competitive; Escherichia coli; Folic Acid; Folic Acid Antagonists; Kinetics; Ligands; Methotrexate; Models, Chemical; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Thermodynamics | 1994 |
Effects of substitution of Thr63 by alanine on the structure and function of Lactobacillus casei dihydrofolate reductase.
Topics: Alanine; Kinetics; Lacticaseibacillus casei; Magnetic Resonance Spectroscopy; Methotrexate; Models, Molecular; Mutagenesis, Site-Directed; NADP; Solutions; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase; Thermodynamics; Threonine | 1994 |
31P-NMR studies of NADPH, NADP+ and the complex of NADPH and methotrexate with Lactobacillus casei dihydrofolate reductase in the solid state.
Topics: Lacticaseibacillus casei; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Phosphorus Isotopes; Tetrahydrofolate Dehydrogenase | 1994 |
Thermodynamic study of dihydrofolate reductase inhibitor selectivity.
Topics: Animals; Escherichia coli; Folic Acid Antagonists; Methotrexate; Mice; NADP; Protein Folding; Temperature; Thermodynamics; Trimethoprim; Trimetrexate | 1994 |
Critical role of phenylalanine 34 of human dihydrofolate reductase in substrate and inhibitor binding and in catalysis.
Topics: Ammonium Sulfate; Catalysis; Chemical Precipitation; Enzyme Stability; Folic Acid; Humans; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Mutagenesis, Site-Directed; NADP; Phenylalanine; Structure-Activity Relationship; Tetrahydrofolate Dehydrogenase | 1994 |
Methotrexate-resistant variants of human dihydrofolate reductase. Effects of Phe31 substitutions.
Topics: Amino Acid Sequence; Crystallography, X-Ray; Drug Resistance; Enzyme Stability; Genetic Therapy; Genetic Variation; Humans; Kinetics; Methotrexate; Models, Structural; Models, Theoretical; Mutagenesis, Site-Directed; NADP; Phenylalanine; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Tetrahydrofolate Dehydrogenase | 1994 |
19F NMR spectroscopy of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase: equilibrium folding and ligand binding studies.
Topics: Base Sequence; Binding Sites; DNA Primers; Escherichia coli; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Protein Conformation; Protein Folding; Tetrahydrofolate Dehydrogenase; Tryptophan; Urea | 1994 |
Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis.
Topics: Circular Dichroism; Cysteine; Escherichia coli; Flow Cytometry; Folic Acid Antagonists; Kinetics; Magnetic Resonance Spectroscopy; Methotrexate; Mutagenesis, Site-Directed; NADP; Protein Conformation; Protein Folding; Tetrahydrofolate Dehydrogenase; Thermodynamics; Urea | 1993 |
Methotrexate resistance in an in vivo mouse tumor due to a non-active-site dihydrofolate reductase mutation.
Topics: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Chromatography, Affinity; DNA Primers; DNA, Neoplasm; Drug Resistance; Enzyme Stability; Escherichia coli; Humans; Kinetics; Leukemia L1210; Methotrexate; Mice; Models, Molecular; Molecular Sequence Data; NADP; Point Mutation; Polymerase Chain Reaction; Protein Conformation; Tetrahydrofolate Dehydrogenase; Time Factors | 1993 |
Dynamics of a flexible loop in dihydrofolate reductase from Escherichia coli and its implication for catalysis.
Topics: Catalysis; Crystallization; Crystallography, X-Ray; Escherichia coli; Folic Acid; Magnetic Resonance Spectroscopy; Methotrexate; Molecular Structure; NADP; Protein Conformation; Tetrahydrofolate Dehydrogenase | 1994 |
Thermodynamic study of folate analogue binding to dihydrofolate reductases from different species.
Topics: Animals; Calorimetry; Cattle; Escherichia coli; Folic Acid Antagonists; Liver; Methotrexate; NADP; Oxidation-Reduction; Species Specificity; Tetrahydrofolate Dehydrogenase; Thermodynamics; Trimethoprim; Trimetrexate | 1993 |
Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro.
Topics: Base Sequence; Folic Acid; Folic Acid Antagonists; Gene Expression Regulation; Humans; Methotrexate; Molecular Sequence Data; NADP; Protein Biosynthesis; Recombinant Proteins; RNA-Binding Proteins; RNA, Messenger; Substrate Specificity; Tetrahydrofolate Dehydrogenase | 1993 |
A new approach to the automatic identification of candidates for ligand receptor sites in proteins: (I). Search for pocket regions.
Topics: Algorithms; Binding Sites; Computer Graphics; Ligands; Methotrexate; Models, Chemical; Models, Molecular; Molecular Structure; NADP; Proteins; Receptors, Drug; Tetrahydrofolate Dehydrogenase | 1993 |
Reduction of quinonoid dihydrobiopterin to tetrahydrobiopterin by nitric oxide synthase.
Topics: Animals; Arginine; Biopterins; Cell Line; Cerebellum; Enzyme Inhibitors; Humans; Indazoles; Kidney; Kinetics; Methotrexate; NADP; Nitric Oxide Synthase; Nitroarginine; Onium Compounds; Oxidation-Reduction; Rats; Recombinant Proteins; Transfection | 1996 |
In search of dihydrofolate reductase.
Topics: Folic Acid; History, 20th Century; Leucovorin; Methotrexate; NADP; Tetrahydrofolate Dehydrogenase | 1996 |
Correlated bond rotations in interactions of arginine residues with ligand carboxylate groups in protein ligand complexes.
Topics: Arginine; Carboxylic Acids; Lacticaseibacillus casei; Ligands; Methotrexate; Molecular Structure; NADP; Optical Rotation; Tetrahydrofolate Dehydrogenase | 1997 |
Domain motions in dihydrofolate reductase: a molecular dynamics study.
Topics: Binding Sites; Chemical Phenomena; Chemistry, Physical; Computer Simulation; Hydrogen Bonding; Lacticaseibacillus casei; Ligands; Methotrexate; Models, Molecular; Molecular Structure; NADP; Protein Binding; Protein Folding; Protein Structure, Secondary; Software; Tetrahydrofolate Dehydrogenase; Water | 1997 |
NMR solution structure of the antitumor compound PT523 and NADPH in the ternary complex with human dihydrofolate reductase.
Topics: Antineoplastic Agents; Binding Sites; Crystallography, X-Ray; Folic Acid Antagonists; Humans; Kinetics; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; NADP; Ornithine; Protein Conformation; Pterins; Tetrahydrofolate Dehydrogenase | 1997 |
The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major.
Topics: Animals; Biopterins; Blotting, Western; Chromatography, Gel; Folic Acid; Folic Acid Antagonists; Humans; Hydrogen-Ion Concentration; Kinetics; Leishmania major; Methotrexate; Models, Chemical; Multienzyme Complexes; NADP; Oxidoreductases; Pteridines; Tetrahydrofolate Dehydrogenase; Thymidylate Synthase | 1997 |
Comparison of ternary crystal complexes of F31 variants of human dihydrofolate reductase with NADPH and a classical antitumor furopyrimidine.
Topics: Antimetabolites, Antineoplastic; Binding Sites; Crystallography, X-Ray; Folic Acid; Folic Acid Antagonists; Humans; Methotrexate; Mutation; NADP; Tetrahydrofolate Dehydrogenase | 1998 |
Light activates reduction of methotrexate by NADPH in the ternary complex with Escherichia coli dihydrofolate reductase.
Topics: Escherichia coli; Folic Acid Antagonists; Humans; In Vitro Techniques; Light; Methotrexate; NADP; Oxidation-Reduction; Photochemistry; Tetrahydrofolate Dehydrogenase | 1999 |
Mutant PTR1 proteins from Leishmania tarentolae: comparative kinetic properties and active-site labeling.
Topics: Animals; Base Sequence; Catalytic Domain; DNA Primers; Drug Resistance; Escherichia coli; Folic Acid Antagonists; Genes, Protozoan; Kinetics; Leishmania; Methotrexate; NADP; Oxidoreductases; Point Mutation; Recombinant Proteins; Substrate Specificity | 1999 |
The crystal structure of dihydrofolate reductase from Thermotoga maritima: molecular features of thermostability.
Topics: Amino Acid Sequence; Binding Sites; Computer Simulation; Crystallography, X-Ray; Dimerization; Enzyme Stability; Folic Acid Antagonists; Ligands; Methotrexate; Models, Molecular; Molecular Sequence Data; NADP; Proline; Protein Structure, Quaternary; Protein Structure, Secondary; Sequence Alignment; Solubility; Static Electricity; Tetrahydrofolate Dehydrogenase; Thermodynamics; Thermotoga maritima | 2000 |
NMR studies of ligand carboxylate group interactions with arginine residues in complexes of Lactobacillus casei dihydrofolate reductase with substrates and substrate analogues.
Topics: 4-Aminopyridine; Amino Acid Sequence; Arginine; Carboxylic Acids; Conserved Sequence; Folic Acid; Glutamates; Hydrogen Bonding; Lacticaseibacillus casei; Ligands; Methotrexate; NADP; Nuclear Magnetic Resonance, Biomolecular; Static Electricity; Tetrahydrofolate Dehydrogenase | 2000 |
Interaction energy analyses of folate analog binding to human dihydrofolate reductase: contribution of the antifolate substructural regions to complex stability.
Topics: Aminopterin; Binding Sites; Energy Metabolism; Folic Acid Antagonists; Humans; Methotrexate; Molecular Structure; NADP; Protein Binding; Software; Tetrahydrofolate Dehydrogenase | 2000 |
Pteridine reductase mechanism correlates pterin metabolism with drug resistance in trypanosomatid parasites.
Topics: Amino Acid Sequence; Animals; Binding Sites; Crystallography, X-Ray; Dihydropteridine Reductase; Drug Resistance; Folic Acid Antagonists; Hydrogen Bonding; Leishmania major; Methotrexate; Models, Molecular; Molecular Sequence Data; NADP; Oxidation-Reduction; Protein Structure, Secondary; Pterins; Selenomethionine; Substrate Specificity; Tetrahydrofolate Dehydrogenase | 2001 |
Highly divergent dihydrofolate reductases conserve complex folding mechanisms.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Apoproteins; Binding Sites; Conserved Sequence; Cyclophilins; Enzyme Stability; Escherichia coli; Evolution, Molecular; Fluorescence; Humans; Kinetics; Lacticaseibacillus casei; Ligands; Methotrexate; Models, Molecular; Molecular Sequence Data; NADP; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Tetrahydrofolate Dehydrogenase; Thermodynamics; Urea | 2002 |
The coordination of the isomerization of a conserved non-prolyl cis peptide bond with the rate-limiting steps in the folding of dihydrofolate reductase.
Topics: Circular Dichroism; Escherichia coli; Fluorescence; Fluorescence Resonance Energy Transfer; Folic Acid Antagonists; Kinetics; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; Models, Chemical; Mutagenesis, Site-Directed; NADP; Protein Binding; Protein Conformation; Protein Folding; Tetrahydrofolate Dehydrogenase; Thermodynamics; Urea | 2003 |
Pharmacophore-based molecular docking to account for ligand flexibility.
Topics: Algorithms; Binding Sites; Binding, Competitive; Databases, Protein; Drug Design; HIV Protease; Ligands; Methotrexate; Methylurea Compounds; Models, Molecular; NADP; Protein Conformation; Proteins; Pyridines; Tetrahydrofolate Dehydrogenase | 2003 |
Automated generation of MCSS-derived pharmacophoric DOCK site points for searching multiconformation databases.
Topics: Binding Sites; Binding, Competitive; Coenzyme A; Databases, Protein; Drug Design; HIV Protease; Ligands; Macrophage Migration-Inhibitory Factors; Methotrexate; Methylurea Compounds; Models, Molecular; NADP; Phenylpyruvic Acids; Proteins; Pyridines; Software; Tetrahydrofolate Dehydrogenase; Transferases (Other Substituted Phosphate Groups) | 2003 |
Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?
Topics: Amino Acid Sequence; Catalysis; Cloning, Molecular; Cold Temperature; Enzyme Activation; Enzyme Stability; Folic Acid Antagonists; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Kinetics; Methotrexate; Molecular Sequence Data; Molecular Weight; Moritella; NADP; Protein Conformation; Protein Denaturation; Sequence Homology, Amino Acid; Temperature; Tetrahydrofolate Dehydrogenase; Trimethoprim | 2003 |
A DIRECT ASSAY FOR DIHYDROFOLATE REDUCTASE IN HUMAN LEUKOCYTES.
Topics: Animals; Chemistry Techniques, Analytical; Folic Acid; Folic Acid Antagonists; Humans; Leukemia; Leukemia L1210; Leukemia, Myeloid; Leukocytes; Methotrexate; Mice; Microchemistry; NADP; Neoplasms; Oxidoreductases; Research; Spectrophotometry; Tetrahydrofolate Dehydrogenase | 1964 |
STUDIES ON THE INHIBITION OF DIHYDROFOLATE REDUCTASE BY THE FOLATE ANTAGONISTS.
Topics: Animals; Carcinoma, Ehrlich Tumor; Enzyme Inhibitors; Folic Acid; Folic Acid Antagonists; Methotrexate; Mice; NADP; Neoplasms, Experimental; Oxidoreductases; Pharmacology; Research; Spectrophotometry; Tetrahydrofolate Dehydrogenase | 1964 |
THE RELATIONSHIP BETWEEN 3 ALPHA-HYDROXYSTEROID NICOTINAMIDE NUCLEOTIDE COENZYME TRANSHYDROGENATION AND BETA-GLUCURONIDASE IN REGENERATING RAT LIVER.
Topics: Androsterone; Coenzymes; Dialysis; Glucuronates; Glucuronidase; Heparin; Hepatectomy; Hydroxysteroids; Lactones; Liver; Liver Extracts; Liver Regeneration; Mercaptopurine; Methotrexate; NAD; NADP; Niacin; Niacinamide; Nucleotides; Oxidoreductases; Perfusion; Pharmacology; Rats; Renal Dialysis; Research; Spectrophotometry | 1964 |
PURIFICATION AND PROPERTIES OF DIHYDROFOLATE REDUCTASE OF SHEEP LIVER.
Topics: Animals; Chromatography; Folic Acid; Liver; Methotrexate; NADP; Oxidoreductases; Pterins; Research; Sheep; Spectrophotometry; Tetrahydrofolate Dehydrogenase | 1964 |
DIHYDROFOLATE REDUCTASE IN GENOTYPICALLY DISTINGUISHABLE AMETHOPTERIN-RESISTANT DIPLOCOCCUS PNEUMONIAE.
Topics: Folic Acid; Genotype; Hydrogen-Ion Concentration; Methotrexate; NADP; Oxidoreductases; Research; Streptococcus pneumoniae; Tetrahydrofolate Dehydrogenase | 1964 |
A NEW PTERIDINE-REQUIRING ENZYME SYSTEM FOR THE OXIDATION OF GLYCERYL ETHERS.
Topics: Alcohols; Aldehydes; Chromatography; Ethers; Fatty Acids; Folic Acid; Glycerol; Glyceryl Ethers; Liver; Methotrexate; NAD; NADP; Oxidation-Reduction; Oxidoreductases; Pteridines; Pterins; Rats; Research; Stearic Acids | 1964 |
ON THE SIMILARITY OF DIHYDROFOLATE REDUCTASES FROM AMETHOPTERIN-SENSITIVE AND AMETHOPTERIN-RESISTANT MOUSE LEUKEMIA (L1210) CELLS.
Topics: Animals; Benzoates; Enzyme Inhibitors; Folic Acid; Leukemia; Leukemia, Experimental; Methotrexate; Mice; NADP; Oxidoreductases; Pharmacology; Research; Sodium Chloride; Tetrahydrofolate Dehydrogenase | 1964 |
THE INHIBITION OF TWO STEROID-ACTIVATED NICOTINAMIDE-ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASES BY FOLIC ACID AND FOLIC ACID ANTIMETABOLITES.
Topics: Aminopterin; Androsterone; Antimetabolites; Enzyme Inhibitors; Estradiol; Folic Acid; Hydrogen-Ion Concentration; Methotrexate; NAD; NADP; Oxidoreductases; Pharmacology; Phosphates; Progesterone; Research; Spectrophotometry; Testosterone | 1965 |
Direct determination of vibrational density of states change on ligand binding to a protein.
Topics: Deuterium Oxide; Escherichia coli; Kinetics; Ligands; Methotrexate; Models, Statistical; NADP; Neutrons; Protein Binding; Proteins; Scattering, Radiation; Tetrahydrofolate Dehydrogenase; Thermodynamics | 2004 |
Inhibition of Leishmania major pteridine reductase by 2,4,6-triaminoquinazoline: structure of the NADPH ternary complex.
Topics: Animals; Binding Sites; Crystallography, X-Ray; Electrons; Enzyme Inhibitors; Hydrogen Bonding; Leishmania major; Methotrexate; Models, Chemical; Models, Molecular; NADP; Oxidoreductases; Protein Conformation; Quinazolines | 2004 |
Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH.
Topics: Amino Acid Sequence; Animals; Arginine; Crystallography, X-Ray; Drug Resistance; Humans; Leucine; Methotrexate; Mice; Models, Molecular; Molecular Sequence Data; NADP; Protein Conformation; Sequence Homology, Amino Acid; Tetrahydrofolate Dehydrogenase | 2005 |
Identification of amino acids required for the functional up-regulation of human dihydrofolate reductase protein in response to antifolate Treatment.
Topics: Amino Acids; Animals; Binding Sites; Blotting, Western; Catalysis; Cell Survival; CHO Cells; Cloning, Molecular; Cricetinae; Cysteine; DNA Primers; Folic Acid Antagonists; Gene Expression Regulation, Enzymologic; Glutamic Acid; Glutathione Transferase; Green Fluorescent Proteins; Humans; Inhibitory Concentration 50; Kinetics; Leucine; Methotrexate; Microscopy, Fluorescence; Models, Biological; Mutagenesis, Site-Directed; Mutation; NADP; Protein Biosynthesis; Recombinant Fusion Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Serine; Spectrophotometry; Tetrahydrofolate Dehydrogenase; Time Factors; Transcription, Genetic; Transfection; Up-Regulation | 2005 |
Ligand binding modulates the mechanical stability of dihydrofolate reductase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Biophysical Phenomena; Biophysics; CHO Cells; Chromatography, Affinity; Cricetinae; Folic Acid; Folic Acid Antagonists; Humans; Ligands; Methotrexate; Microscopy, Atomic Force; NAD; NADP; Niacinamide; Proteasome Endopeptidase Complex; Protein Binding; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Protein Transport; Spectrophotometry; Stress, Mechanical; Tensile Strength; Tetrahydrofolate Dehydrogenase; Thermodynamics | 2005 |
Crystal structure of Trypanosoma cruzi pteridine reductase 2 in complex with a substrate and an inhibitor.
Topics: Amino Acid Sequence; Animals; Binding Sites; Crystallography, X-Ray; Enzyme Inhibitors; Folic Acid; Isoenzymes; Macromolecular Substances; Methotrexate; Models, Molecular; Molecular Sequence Data; Molecular Structure; NADP; Oxidoreductases; Protein Structure, Quaternary; Protozoan Proteins; Sequence Alignment; Trypanosoma cruzi | 2005 |
Fingerprinting DHFR in single-molecule AFM studies.
Topics: Animals; Biophysical Phenomena; Biophysics; CHO Cells; Chromatography, Affinity; Cricetinae; Cricetulus; Folic Acid; Folic Acid Antagonists; Humans; Ligands; Methotrexate; Microscopy, Atomic Force; NAD; NADP; Niacinamide; Peptide Mapping; Proteasome Endopeptidase Complex; Protein Binding; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Protein Transport; Spectrophotometry; Stress, Mechanical; Tensile Strength; Tetrahydrofolate Dehydrogenase; Thermodynamics | 2006 |
X-ray structure of the ternary MTX.NADPH complex of the anthrax dihydrofolate reductase: a pharmacophore for dual-site inhibitor design.
Topics: Bacillus anthracis; Crystallography, X-Ray; Drug Design; Methotrexate; Models, Molecular; NADP; Protein Structure, Secondary; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase | 2009 |
Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase.
Topics: Catalytic Domain; Colorimetry; Crystallography, X-Ray; Cytochromes c; Kinetics; Methotrexate; NADP; Oxidation-Reduction; Oxidoreductases; Tetrahydrofolate Dehydrogenase; Trypanosoma brucei brucei | 2010 |
Nuclear magnetic resonance study of the role of M42 in the solution dynamics of Escherichia coli dihydrofolate reductase.
Topics: Escherichia coli Proteins; Methionine; Methotrexate; NADP; Nuclear Magnetic Resonance, Biomolecular; Protein Binding; Protein Structure, Secondary; Tetrahydrofolate Dehydrogenase; Thermodynamics; Tryptophan | 2010 |
A second target of benzamide riboside: dihydrofolate reductase.
Topics: Adenine Nucleotides; Benzamides; Cell Line, Tumor; Down-Regulation; Drug Resistance, Neoplasm; Humans; IMP Dehydrogenase; Methotrexate; Molecular Targeted Therapy; NADP; Nucleosides; Phosphotransferases (Alcohol Group Acceptor); Precursor Cell Lymphoblastic Leukemia-Lymphoma; Tetrahydrofolate Dehydrogenase | 2012 |
Enhanced degradation of dihydrofolate reductase through inhibition of NAD kinase by nicotinamide analogs.
Topics: Biological Transport; Cell Line, Tumor; Half-Life; Humans; Methotrexate; NADP; Niacinamide; Phosphotransferases (Alcohol Group Acceptor); Proteolysis; Tetrahydrofolate Dehydrogenase; Transcription, Genetic; Up-Regulation | 2013 |
Contribution of serine, folate and glycine metabolism to the ATP, NADPH and purine requirements of cancer cells.
Topics: Adenosine Triphosphate; Amino Acid Oxidoreductases; Animals; Carrier Proteins; Cell Line, Tumor; Embryonic Stem Cells; Energy Metabolism; Fatty Acids; Female; Folic Acid; Gene Expression Regulation, Neoplastic; Glycine; Humans; Male; Metabolic Flux Analysis; Metabolic Networks and Pathways; Methotrexate; Mice; Multienzyme Complexes; NADP; Neoplasms; Protein Biosynthesis; Purines; Serine; Transferases | 2013 |
Structural Characterization of Dihydrofolate Reductase Complexes by Top-Down Ultraviolet Photodissociation Mass Spectrometry.
Topics: Binding Sites; Escherichia coli; Folic Acid Antagonists; Ligands; Mass Spectrometry; Methotrexate; Models, Molecular; NADP; Photochemical Processes; Tetrahydrofolate Dehydrogenase; Ultraviolet Rays | 2015 |
Oxidative stress inhibits distant metastasis by human melanoma cells.
Topics: Animals; Antioxidants; Female; Folic Acid; Gene Knockdown Techniques; Humans; Male; Melanoma; Methotrexate; Methylenetetrahydrofolate Dehydrogenase (NADP); Mice; Mice, Inbred NOD; Mice, SCID; Minor Histocompatibility Antigens; NADP; Neoplasm Metastasis; Neoplasm Transplantation; Oxidative Stress; Oxidoreductases Acting on CH-NH Group Donors | 2015 |
Screening of protein-ligand interactions under crude conditions by native mass spectrometry.
Topics: Binding Sites; Drug Evaluation, Preclinical; Escherichia coli; Escherichia coli Proteins; Folic Acid Antagonists; Methotrexate; NADP; Protein Binding; Spectrometry, Mass, Electrospray Ionization; Tetrahydrofolate Dehydrogenase | 2020 |
The SecA motor generates mechanical force during protein translocation.
Topics: Cell Membrane; Escherichia coli; Escherichia coli Proteins; Lipid Bilayers; Membrane Transport Proteins; Methotrexate; NADP; Protein Transport; Protein Unfolding; SEC Translocation Channels; SecA Proteins; Stress, Mechanical; Tetrahydrofolate Dehydrogenase | 2020 |
NADPH metabolism determines the leukemogenic capacity and drug resistance of AML cells.
Topics: Animals; Bone Marrow; Drug Resistance, Neoplasm; Humans; Leukemia, Myeloid, Acute; Methotrexate; Mice; NADP | 2022 |