methoprene and 4-(2-(5-6-7-8-tetrahydro-5-5-8-8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic-acid

methoprene has been researched along with 4-(2-(5-6-7-8-tetrahydro-5-5-8-8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic-acid* in 1 studies

Other Studies

1 other study(ies) available for methoprene and 4-(2-(5-6-7-8-tetrahydro-5-5-8-8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic-acid

Article	Year
Retinoic acid improves a hybridoma culture in a fructose-based medium by up-regulation of fructose incorporation via retinoid nuclear receptors. Bioscience, biotechnology, and biochemistry, 2006, Volume: 70, Issue:9 Fructose was focused on as an alternative sugar source to glucose in a hybridoma culture medium because it decreases lactate production during cultivation, leading to cell and product stability. But, not all human hybridoma cell lines grew well in a fructose-based serum-free medium. We found that the addition of all-trans-retinoic acid to the fructose-based medium improved the growth and monoclonal antibody production of hybridoma cell lines by up-regulation of fructose incorporation that represented increased expression of the fructose transporter, GLUT5. Selective activation of retinoid nuclear receptor by synthetic ligands showed that both retinoic acid receptors and retinoid X receptors might be related to the improvement of the fructose-based hybridoma culture. This study might be applicable to cell cultures susceptible to lactate and pH changes as well as hybridoma cultures. Topics: Antibodies, Monoclonal; Antibody Formation; Benzoates; Cell Growth Processes; Cell Line; Culture Media; Fructose; Glucose Transporter Type 5; Humans; Hybridomas; Lactic Acid; Methoprene; Receptors, Retinoic Acid; Retinoid X Receptors; Retinoids; Reverse Transcriptase Polymerase Chain Reaction; RNA; Tetrahydronaphthalenes; Tretinoin; Up-Regulation	2006

Article

Year

Retinoic acid improves a hybridoma culture in a fructose-based medium by up-regulation of fructose incorporation via retinoid nuclear receptors.

Bioscience, biotechnology, and biochemistry, 2006, Volume: 70, Issue:9

Fructose was focused on as an alternative sugar source to glucose in a hybridoma culture medium because it decreases lactate production during cultivation, leading to cell and product stability. But, not all human hybridoma cell lines grew well in a fructose-based serum-free medium. We found that the addition of all-trans-retinoic acid to the fructose-based medium improved the growth and monoclonal antibody production of hybridoma cell lines by up-regulation of fructose incorporation that represented increased expression of the fructose transporter, GLUT5. Selective activation of retinoid nuclear receptor by synthetic ligands showed that both retinoic acid receptors and retinoid X receptors might be related to the improvement of the fructose-based hybridoma culture. This study might be applicable to cell cultures susceptible to lactate and pH changes as well as hybridoma cultures.

Topics: Antibodies, Monoclonal; Antibody Formation; Benzoates; Cell Growth Processes; Cell Line; Culture Media; Fructose; Glucose Transporter Type 5; Humans; Hybridomas; Lactic Acid; Methoprene; Receptors, Retinoic Acid; Retinoid X Receptors; Retinoids; Reverse Transcriptase Polymerase Chain Reaction; RNA; Tetrahydronaphthalenes; Tretinoin; Up-Regulation

2006