methanol has been researched along with alcohol oxidase in 114 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 13 (11.40) | 18.7374 |
| 1990's | 30 (26.32) | 18.2507 |
| 2000's | 36 (31.58) | 29.6817 |
| 2010's | 29 (25.44) | 24.3611 |
| 2020's | 6 (5.26) | 2.80 |
| Authors | Studies |
|---|---|
| Ballantine, SP; Clare, JJ; Rayment, FB; Romanos, MA; Sreekrishna, K | 1 |
| Sakai, Y; Tani, Y | 1 |
| Harder, W; Keizer, I; Roggenkamp, R; Veenhuis, M | 1 |
| Cregg, JM; Liu, H; McCollum, D; Tan, X; Veenhuis, M | 1 |
| Hofmann, KH; Polnisch, E | 1 |
| Bystrykh, LV; Dijkhuizen, L; Harder, W | 1 |
| Harder, W; van der Klei, IJ; Veenhuis, M | 2 |
| Faridnia, MH; Gyilbault, GG; Lubrano, GJ; Palleschi, G | 1 |
| Dorizzi, R; Ghielmi, S; Marigo, M; Tagliaro, F | 1 |
| Dorizzi, R; Marigo, M; Schiavon, G; Tagliaro, F | 1 |
| Wegner, GH | 1 |
| van der Klei, IJ; Veenhuis, M | 1 |
| Vinet, B | 2 |
| Distel, B; Tabak, HF; Van der Leÿ, I; Veenhuis, M | 1 |
| Brust, PF; Cregg, JM; Gingeras, TR; Stillman, CA; Tschopp, JF | 1 |
| Geissler, J; Ghisla, S; Kroneck, PM | 1 |
| Cartledge, TG; Hill, DJ; Jenkins, RO; Lloyd, D | 1 |
| Brust, PF; Ellis, SB; Gingeras, TR; Harpold, MM; Koutz, PJ; Waters, AF | 1 |
| Herzberg, GR; Rogerson, M | 1 |
| Hann, AC; Hill, DJ; Lloyd, D | 1 |
| Atherton, JP; Donahue, PN; Goodman, JM; Scott, CW | 1 |
| Clark, LC; Grooms, TA; Moore, PE; Noyes, LK | 1 |
| Derelanko, P; Hou, CT; Laskin, AI; Patel, RN | 1 |
| Harder, W; Mayer, F; van Dijken, JP; Veenhuis, M | 1 |
| Hughes, JA; Jay, M | 1 |
| Dunn, WA; Tuttle, DL | 1 |
| Bardeletti, G; Carillon, C | 1 |
| Fazi, A; Magnani, M; Mancini, U; Mangani, F; Rossi, L | 1 |
| Dunn, WA; Lewin, AS; Tuttle, DL | 1 |
| Cregg, JM; Eyers, M; Haima, P; Harder, W; Sulter, GJ; Tan, X; Titorenko, VI; van der Klei, IJ; Veenhuis, M; Waterham, HR | 1 |
| Akiyama, M; Kato, N; Kondoh, H; Sakai, Y; Shibano, Y | 1 |
| Cremata, J; Delgado, JM; Grillo, JM; Herrera, LS; Montesinos, R; Muzio, V; Narciandi, RE; Roca, H; Rodriguez, E; Rodriguez, L | 1 |
| Cregg, JM; Hendricks, M; Neiditch, BR; Raschke, WC | 1 |
| Cregg, JM; Digan, ME; Koutz, PJ; Lair, SV; Waterham, HR | 1 |
| Bukowski, T; Ching, AF; Holderman, SD; Raymond, CK; Stamm, MR; Vanaja, E | 1 |
| Glick, BS; O'Connor, J; Rossanese, OW; Sears, IB | 1 |
| Kato, N; Matsuo, H; Sakai, Y; Yurimoto, H | 1 |
| Alamäe, T; Liiv, L | 1 |
| Gorton, L; Lidén, H; Marko-Varga, G; Vijayakumar, AR | 1 |
| Cereghino, JL; Cregg, JM; Fayura, LR; Johnson, MA; Ksheminska, GP; Kulachkovsky, AR; Sibirny, AA; Stasyk, OV; Veenhuis, M; Waterham, HR | 1 |
| Kato, N; Mukaiyama, H; Nakagawa, T; Sakai, Y; Yurimoto, H | 1 |
| Faber, KN; Kiel, JA; Salomons, FA; van der Klei, IJ; Veenhuis, M | 1 |
| Domínguez, E; Katakis, I; Narváez, A; Popescu, IC; Suárez, G | 1 |
| Kato, N; Komeda, T; Kondo, K; Lim, CR; Nakagawa, T; Sakai, Y; Yurimoto, H | 1 |
| Kato, N; Miyaji, T; Nakagawa, T; Sakai, Y; Tomizuka, N; Yurimoto, H | 1 |
| Cao, ZA; Gu, XY; Li, Q | 1 |
| Lahtchev, KL; Semenova, VD; Tolstorukov, II; van der Klei, I; Veenhuis, M | 1 |
| Coşkun, H; Gülce, A; Gülce, H; Kavanoz, M; Yildiz, A | 1 |
| Kato, N; Miyaji, T; Mizumura, T; Mukaiyama, H; Nakagawa, T; Sakai, Y; Tomizuka, N; Yurimoto, H | 1 |
| Kiel, JA; Komduur, JA; Leão, AN; Monastyrska, I; Veenhuis, M | 1 |
| Ehn, M; Gharizadeh, B; Hober, S; Nourizad, N; Nyrén, P | 1 |
| Kram, AM; Lahchev, KL; van der Klei, IJ; van Dijk, R; Veenhuis, M | 1 |
| Mergler, M; Wolf, K; Zimmermann, M | 1 |
| Hong, B; Hu, J; Li, Y | 1 |
| Cregg, JM; Komduur, J; Sibirny, AA; Stasyk, OG; Stasyk, OV; Veenhuis, M | 1 |
| Fukusaki, E; Hasunuma, T; Kobayashi, A; Kuwabata, S | 1 |
| Kiel, JA; Lahtchev, K; Ozimek, P; van der Klei, IJ; Veenhuis, M | 1 |
| Anthon, GE; Barrett, DM | 1 |
| Grsic-Rausch, S; Rausch, T | 1 |
| Fujimura, S; Ito, T; Miyaji, T; Nakagawa, T; Tomizuka, N; Yamada, K | 1 |
| Dorgai, L; Kucsera, J; Rubiera, R; Szamecz, B; Urbán, G | 1 |
| Fujimura, S; Inagaki, A; Ito, T; Kato, N; Miyaji, T; Nakagawa, T; Sakai, Y; Tomizuka, N; Yurimoto, H | 1 |
| Cregg, JM; de la Cruz, BJ; Godfrey, L; Johnson, S; Khuongsathiene, S; Lin-Cereghino, GP; Lin-Cereghino, J; Subramani, S; Tolstorukov, I; Veenhuis, M; Yan, M | 1 |
| Gayda, GZ; Gonchar, MV; Morozova, OV; Nikitina, OV; Pavlishko, HM; Shleev, SV; Shumakovich, GP | 1 |
| Chen, CS; Choi, MM; Wu, XJ; Wu, XM | 1 |
| Bardiya, N | 1 |
| Sakai, Y; van der Klei, IJ; Veenhuis, M; Yurimoto, H | 1 |
| Jungo, C; Marison, I; von Stockar, U | 2 |
| Klompmaker, SH; Ozimek, PZ; van der Klei, IJ; Veenhuis, M; Visser, N | 1 |
| Fujimura, S; Ito, T; Matsufuji, Y; Miyaji, T; Nakagawa, T; Tomizuka, N | 1 |
| Fujimura, S; Ito, T; Matsufuji, Y; Miyaji, T; Nakagawa, T; Takano, K; Tanaka, N; Tomizuka, N; Uchino, M | 1 |
| Enfors, SO; Jahic, M; Montesinos, JL; Stahn, R; Surribas, A; Valero, F | 1 |
| Ah Kang, H; Sibirny, AA; Stasyk, OG; Stasyk, OV; van Zutphen, T; Veenhuis, M | 1 |
| Brewer, KL; Early, J; Hack, JB | 1 |
| Chu, J; Zou, M | 1 |
| Kraut, JA; Sachs, G; Shin, JM | 1 |
| Glieder, A; Peter Luef, K; Pscheidt, B; Schroer, K; Stefan Hartner, F | 1 |
| Chen, J; Du, G; Hua, Z; Li, J; Wang, Y; Wang, Z; Zhang, D | 1 |
| Shen, CH; Yeh, KW | 1 |
| Avital-Shmilovici, M; Shabat, D | 1 |
| Kuriyama, M; Sakai, Y; Sasano, Y; Yurimoto, H | 1 |
| Dai, K; Jin, H; Li, Z; Liu, G; Shi, Z; Wang, H | 1 |
| Sakai, Y; Yurimoto, H; Zhai, Z | 1 |
| Chiba, Y; Eurwilaichitr, L; Promdonkoy, P; Roongsawang, N; Tanapongpipat, S; Tirasophon, W; Watanabe, T | 1 |
| Kumar, NV; Rangarajan, PN | 1 |
| Padkina, MV; Rumiantsev, AM; Sambuk, EV | 1 |
| Chu, J; Hu, X; Zhang, S; Zhuang, Y | 1 |
| Eurwilaichitr, L; Promdonkoy, P; Roongsawang, N; Tanapongpipat, S; Tirasophon, W | 1 |
| Barbora, L; Das, M; Das, P; Goswami, P | 1 |
| Ata, Ö; Boy, E; Çalık, P; Güneş, H | 1 |
| Fujiki, Y; Kishimoto, M; Kumada, Y | 1 |
| Capone, S; Herwig, C; Horvat, J; Spadiut, O | 1 |
| Kenzom, T; Mishra, S; Srivastava, P | 1 |
| Bai, P; Cai, M; Shen, W; Shi, L; Wang, J; Wang, Q; Wang, X; Zhang, Y; Zhou, M; Zhou, X | 1 |
| Bruhn, S; Flaschel, E; Friehs, K; Müller, JM; Risse, JM | 1 |
| Hayakawa, T; Mizumura, T; Nakagawa, T; Ozawa, S; Takeuchi, A; Tomizuka, N; Wakayama, K; Yamaguchi, S | 1 |
| Cai, M; Huang, M; Kong, C; Liu, Y; Shen, W; Wang, X; Xue, Y; Zhang, Y; Zhou, M; Zhou, X | 1 |
| Albiol, J; Cámara, E; Ferrer, P; Gasser, B; Landes, N; Mattanovich, D | 1 |
| Dragosits, M; Gerner, SM; Graf, AB; Mattanovich, D; Moser, JW; Prielhofer, R; Wilson, IB | 1 |
| Barroso, J; Briz, N; Díez-Buitrago, B; Möller, M; Pavlov, V; Saa, L | 1 |
| Cai, M; Shi, L; Wang, J; Wang, X; Zhang, Y; Zhou, X | 1 |
| Cai, M; Qi, F; Shi, L; Wang, J; Wang, X; Zhang, P; Zhang, Y; Zhou, X | 1 |
| Champreda, V; Kanokratana, P; Laobuthee, A; Laosiripojana, N; Mangkorn, N; Roongsawang, N | 1 |
| Spadiut, O; Wurm, DJ | 1 |
| Erden-Karaoğlan, F; İnan, M; Karaoğlan, M; Yılmaz, S | 1 |
| Garcia-Ortega, X; Garrigós-Martínez, J; Gasset-Franch, A; Montesinos-Seguí, JL; Nieto-Taype, MA; Valero, F | 1 |
| Bulbotka, N; Dmytruk, K; Dmytruk, O; Semkiv, M; Sibirny, A; Zazulya, A | 1 |
| Becker, T; Brunner, V; Geier, D; Siegl, M | 1 |
| Altmann, F; Gasser, B; Hann, S; Maresch, D; Mattanovich, D; Troyer, C; Zavec, D | 1 |
| Gayda, GZ; Gonchar, MV; Klepach, HM; Smutok, OV; Zakalska, OM; Zakalskiy, AE | 1 |
| Chiba, Y; Gao, XD; Komatsuzaki, A; Umemura, M; Yoko-O, T; Yoshihara, E; Zhao, S | 1 |
4 review(s) available for methanol and alcohol oxidase
| Article | Year |
|---|---|
Biosynthesis and assembly of alcohol oxidase, a peroxisomal matrix protein in methylotrophic yeasts: a review.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Crystallization; Flavin-Adenine Dinucleotide; Gene Expression Regulation, Fungal; Genes, Fungal; Methanol; Microbodies; Molecular Sequence Data; Yeasts | 1991 |
Emerging applications of the methylotrophic yeasts.
Topics: Alcohol Oxidoreductases; Dietary Proteins; Fermentation; Fungal Proteins; Gene Expression; Methanol; Recombinant Proteins; Yeasts | 1990 |
The significance of peroxisomes in methanol metabolism in methylotrophic yeast.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Catalase; Formaldehyde; Gene Expression Regulation, Fungal; Glutathione Peroxidase; Methanol; Peroxisomes; Pichia; Thiolester Hydrolases | 2006 |
Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application.
Topics: Alcohol Oxidoreductases; Batch Cell Culture Techniques; Biosensing Techniques; Chromatography, Ion Exchange; Cloning, Molecular; Formaldehyde; Fungal Proteins; Methanol; Saccharomycetales | 2021 |
110 other study(ies) available for methanol and alcohol oxidase
| Article | Year |
|---|---|
High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene.
Topics: Alcohol Oxidoreductases; Animals; Base Sequence; Cloning, Molecular; DNA; Gene Amplification; Genetic Vectors; Methanol; Mice; Molecular Sequence Data; Peptide Fragments; Pichia; Tetanus; Tetanus Toxin; Tetanus Toxoid; Transformation, Genetic; Vaccines, Synthetic | 1991 |
Cloning and sequencing of the alcohol oxidase-encoding gene (AOD1) from the formaldehyde-producing asporogeneous methylotrophic yeast, Candida boidinii S2.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Base Sequence; Blotting, Northern; Candida; Cloning, Molecular; Gene Expression Regulation, Fungal; Methanol; Microbodies; Molecular Sequence Data; Pichia; Regulatory Sequences, Nucleic Acid | 1992 |
Location of catalase in crystalline peroxisomes of methanol-grown Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Catalase; Crystallization; Culture Media; Methanol; Microbodies; Microscopy, Electron; Pichia | 1992 |
An efficient screen for peroxisome-deficient mutants of Pichia pastoris.
Topics: Acyl-CoA Oxidase; Alcohol Oxidoreductases; Methanol; Microbodies; Mutation; Oleic Acid; Oleic Acids; Oxidoreductases; Pichia | 1992 |
Catabolite inactivation, cyclic AMP and protein phosphorylation in the methylotrophic yeast Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Cyclic AMP; Electrophoresis, Polyacrylamide Gel; Fructose-Bisphosphatase; Fungal Proteins; Malate Dehydrogenase; Methanol; NADP; Phosphoenolpyruvate Carboxykinase (GTP); Phosphorylation; Pichia; Protein Kinases; Staining and Labeling | 1991 |
Modification of flavin adenine dinucleotide in alcohol oxidase of the yeast Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Candida; Flavin-Adenine Dinucleotide; Formaldehyde; Fungal Proteins; Hydroxylamine; Hydroxylamines; Kinetics; Methanol; Pichia | 1991 |
Methanol metabolism in a peroxisome-deficient mutant of Hansenula polymorpha: a physiological study.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Aldehyde-Ketone Transferases; Catalase; Formaldehyde; Formate Dehydrogenases; Formates; Glutathione Reductase; Immunohistochemistry; Methanol; Microbodies; Microscopy, Electron; Mutation; Oxidation-Reduction; Oxygen Consumption; Pichia; Transferases | 1991 |
Amperometric alcohol electrode with extended linearity and reduced interferences.
Topics: Alcohol Oxidoreductases; Cross-Linking Reagents; Electrochemistry; Electrodes; Enzymes, Immobilized; Ethanol; Glutaral; Hydrogen Peroxide; Membranes, Artificial; Methanol; Polycarboxylate Cement | 1991 |
Direct injection high-performance liquid chromatographic method with electrochemical detection for the determination of ethanol and methanol in plasma using an alcohol oxidase reactor.
Topics: Alcohol Oxidoreductases; Candida; Chromatography, High Pressure Liquid; Electrochemistry; Electrodes; Ethanol; Hydrogen Peroxide; Methanol; Platinum; Reproducibility of Results | 1991 |
Development of post-column enzymic reactors with immobilized alcohol oxidase for use in the high-performance liquid chromatographic assay of alcohols with electrochemical detection.
Topics: Alcohol Oxidoreductases; Alcohols; Chromatography, High Pressure Liquid; Electrochemistry; Enzymes, Immobilized; Ethanol; Indicators and Reagents; Methanol; Microchemistry; Saccharomyces cerevisiae | 1991 |
Cytochemical staining methods for localization of key enzymes of methanol metabolism in Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Catalase; Histocytochemistry; Immunohistochemistry; Methanol; Pichia; Protoplasts | 1990 |
Enzymic methanol determination: toxic concentrations of ethanol may give positive values.
Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Ethanol; False Positive Reactions; Humans; Methanol | 1988 |
Alcohol oxidase expressed under nonmethylotrophic conditions is imported, assembled, and enzymatically active in peroxisomes of Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Biological Transport; Blotting, Southern; Culture Media; DNA Probes; DNA, Fungal; Enzyme Activation; Gene Expression Regulation; Glucose; Histocytochemistry; Methanol; Microbodies; Pichia; Plasmids; Recombinant Fusion Proteins; Restriction Mapping; Saccharomycetales; Transfection | 1988 |
Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Bacterial Proteins; beta-Galactosidase; Escherichia coli; Fungal Proteins; Galactosidases; Gene Expression Regulation; Genes, Fungal; Methanol; Pichia; Promoter Regions, Genetic; Recombinant Fusion Proteins; Recombinant Proteins; Saccharomyces cerevisiae; Saccharomycetales; Transferases | 1987 |
An enzymic assay for the specific determination of methanol in serum.
Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Formaldehyde; Humans; Hydrogen-Ion Concentration; Methanol | 1987 |
Flavin-dependent alcohol oxidase from yeast. Studies on the catalytic mechanism and inactivation during turnover.
Topics: Aerobiosis; Alcohol Oxidoreductases; Anaerobiosis; Candida; Catalase; Catalysis; Deuterium; Flavins; Hydrogen Peroxide; Kinetics; Methanol; Models, Chemical; Substrate Specificity | 1986 |
Changes in proteinase activities and subcellular distribution during inactivation of alcohol oxidase in Candida boidinii.
Topics: Alcohol Oxidoreductases; Candida; Centrifugation, Density Gradient; Chloroquine; Endopeptidases; Ethanol; Methanol; Pepstatins; Phenylmethylsulfonyl Fluoride; Subcellular Fractions | 1986 |
Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Ascomycota; Base Sequence; DNA; DNA, Fungal; Gene Expression Regulation; Genes, Fungal; Genes, Regulator; Methanol; Pichia; Promoter Regions, Genetic; Transcription, Genetic | 1985 |
Use of alcohol oxidase to measure the methanol produced during the hydrolysis of D- and L-methyl-3-hydroxybutyric acid.
Topics: Alcohol Oxidoreductases; Hydrolysis; Hydroxybutyrates; Methanol; Peroxidases; Spectrophotometry | 1985 |
Degradative inactivation of the peroxisomal enzyme, alcohol oxidase, during adaptation of methanol-grown Candida boidinii to ethanol.
Topics: Adaptation, Physiological; Alcohol Oxidoreductases; Bacterial Proteins; Candida; Cytochromes; Ethanol; Flavins; Methanol; Microbodies; Microscopy, Electron; Spectrophotometry | 1985 |
Alcohol oxidase assembles post-translationally into the peroxisome of Candida boidinii.
Topics: Alcohol Oxidoreductases; Candida; Cell Fractionation; Kinetics; Macromolecular Substances; Methanol; Microbodies; Microscopy, Electron; Protein Biosynthesis; Protein Processing, Post-Translational; Spheroplasts | 1984 |
Direct rapid electroenzymatic sensor for measuring alcohol in whole blood and fermentation products.
Topics: Alcohol Oxidoreductases; Animals; Electrodes; Enzymes, Immobilized; Ethanol; Methanol; Mice; Polarography | 1984 |
Microbial oxidation of methanol: properties of crystallized alcohol oxidase from a yeast, Pichia sp.
Topics: Alcohol Oxidoreductases; Ascomycota; Crystallization; Flavins; Kinetics; Methanol; Pichia; Spectrophotometry; Substrate Specificity | 1981 |
Substructure of crystalline peroxisomes in methanol-grown Hansenula polymorpha: evidence for an in vivo crystal of alcohol oxidase.
Topics: Alcohol Oxidoreductases; Ascomycota; Catalase; Histocytochemistry; Macromolecular Substances; Methanol; Microbodies; Organoids; Pichia; Spheroplasts | 1981 |
Preparation of [11C]formaldehyde using a hollow fiber membrane bioreactor.
Topics: Alcohol Oxidoreductases; Carbon Radioisotopes; Catalase; Enzymes, Immobilized; Formaldehyde; Methanol | 1995 |
Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Autophagy; Cytosol; Ethanol; Formate Dehydrogenases; Fungal Proteins; Genes, Fungal; Glucose; Kinetics; Membrane Fusion; Methanol; Microbodies; Microscopy, Electron; Mutagenesis; Pichia; Vacuoles | 1995 |
Protease determination using an optimized alcohol enzyme electrode.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Calibration; Candida; Endopeptidases; Enzymes, Immobilized; Ethanol; Hydrogen Peroxide; Hydrogen-Ion Concentration; Methanol; Temperature; Trypsin | 1993 |
Methanol detoxification by enzyme-loaded erythrocytes.
Topics: Alcohol Oxidoreductases; Animals; Erythrocyte Aging; Erythrocytes; Ethanol; Humans; Methanol; Methemoglobin; Mice; Pichia | 1993 |
Selective autophagy of peroxisomes in methylotrophic yeasts.
Topics: Alcohol Oxidoreductases; Autophagy; Glucose; Methanol; Microbodies; Pichia; Vacuoles | 1993 |
Characterization of peroxisome-deficient mutants of Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Alleles; Catalase; Crosses, Genetic; Cytosol; Fungal Proteins; Genes, Dominant; Genes, Fungal; Genes, Recessive; Methanol; Microbodies; Pichia; Transferases | 1995 |
High-level secretion of fungal glucoamylase using the Candida boidinii gene expression system.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Base Sequence; Candida; Culture Media; Enzyme Stability; Fungal Proteins; Genetic Vectors; Glucan 1,4-alpha-Glucosidase; Glycosylation; Methanol; Molecular Sequence Data; Protein Sorting Signals; Recombinant Fusion Proteins; Rhizopus; Saccharomyces cerevisiae; Transformation, Genetic | 1996 |
Invertase secretion in Hansenula polymorpha under the AOX1 promoter from Pichia pastoris.
Topics: Alcohol Oxidoreductases; beta-Fructofuranosidase; Cell Compartmentation; Culture Media; Gene Expression Regulation, Fungal; Genes, Fungal; Genetic Vectors; Glycerol; Glycoside Hydrolases; Methanol; Pichia; Promoter Regions, Genetic; Recombinant Fusion Proteins; Saccharomyces cerevisiae | 1996 |
Inducible expression of a heterologous protein in Hansenula polymorpha using the alcohol oxidase 1 promoter of Pichia pastoris.
Topics: Alcohol Oxidoreductases; Aprotinin; Base Sequence; Cloning, Molecular; DNA, Fungal; Gene Expression Regulation, Fungal; Humans; Methanol; Molecular Sequence Data; Pichia; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Species Specificity; Transcription, Genetic | 1996 |
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Bacterial Proteins; Base Sequence; beta-Lactamases; Biological Transport; Cloning, Molecular; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Reporter; Genetic Vectors; Glucose; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerol; Methanol; Microbodies; Molecular Sequence Data; Oleic Acid; Pichia; Promoter Regions, Genetic; Recombinant Proteins; Sequence Analysis, DNA; Transcription, Genetic | 1997 |
Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Aspartic Acid Endopeptidases; Base Sequence; Cloning, Molecular; Endopeptidases; Fermentation; Gene Expression; Genes, Fungal; Glutamate Decarboxylase; Humans; Methanol; Molecular Sequence Data; Mutagenesis; Pichia; Plasmids; Recombinant Proteins; Sequence Analysis, DNA; Transformation, Genetic; Vacuoles | 1998 |
A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Aminohydrolases; Enzyme Induction; Fungal Proteins; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Reporter; Genetic Vectors; Glucose; Glucuronidase; Glyceraldehyde-3-Phosphate Dehydrogenases; GTP Phosphohydrolases; GTP-Binding Proteins; Mannitol; Methanol; Molecular Sequence Data; Pichia; Promoter Regions, Genetic; Pyrophosphatases; rab GTP-Binding Proteins; Saccharomyces cerevisiae Proteins; Transcription Factors | 1998 |
Regulation of peroxisomal proteins and organelle proliferation by multiple carbon sources in the methylotrophic yeast, Candida boidinii.
Topics: Acyl-CoA Oxidase; Alanine; Alcohol Oxidoreductases; Blotting, Northern; Blotting, Western; Candida; Catalase; D-Amino-Acid Oxidase; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Fungal; Glucose; Green Fluorescent Proteins; Indicators and Reagents; Luminescent Proteins; Methanol; Microbodies; Microscopy, Fluorescence; Oleic Acid; Organelles; Oxidoreductases; Polymerase Chain Reaction; RNA, Fungal | 1998 |
Glucose repression of maltase and methanol-oxidizing enzymes in the methylotrophic yeast Hansenula polymorpha: isolation and study of regulatory mutants.
Topics: Alcohol Oxidoreductases; alpha-Glucosidases; Bacterial Proteins; Culture Media; Enzyme Repression; Gene Expression Regulation, Fungal; Glucose; Methanol; Oxidation-Reduction; Pichia; Repressor Proteins; Substrate Specificity | 1998 |
Rapid alcohol determination in plasma and urine by column liquid chromatography with biosensor detection.
Topics: Acids; Alcohol Oxidoreductases; Aldehydes; Biosensing Techniques; Calibration; Chromatography, Liquid; Drug Stability; Ethanol; Female; Fungi; Horseradish Peroxidase; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Methanol; Pregnancy; Sensitivity and Specificity | 1998 |
Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Alleles; Base Sequence; DNA Primers; Genes, Fungal; Genetic Complementation Test; Methanol; Microbodies; Microscopy, Electron; Mutation; Pichia; Propanols; Selection, Genetic | 1999 |
Alcohol oxidase hybrid oligomers formed in vivo and in vitro.
Topics: Alcohol Oxidoreductases; Candida; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme Induction; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal; Genetic Complementation Test; Isoenzymes; Methanol; Molecular Sequence Data; Molecular Weight; Mutagenesis, Insertional; Pichia; Protein Binding | 1999 |
Overproduction of Pex5p stimulates import of alcohol oxidase and dihydroxyacetone synthase in a Hansenula polymorpha Pex14 null mutant.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Blotting, Western; Carrier Proteins; Endopeptidases; Fungal Proteins; Glycerol; Immunohistochemistry; Membrane Proteins; Membrane Transport Proteins; Methanol; Microscopy, Electron; Models, Biological; Mutagenesis; Peroxins; Peroxisome-Targeting Signal 1 Receptor; Pichia; Receptors, Cytoplasmic and Nuclear; Repressor Proteins; Saccharomyces cerevisiae Proteins; Sucrose | 2000 |
Reagentless biosensors based on self-deposited redox polyelectrolyte-oxidoreductases architectures.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Electrochemistry; Fructose; Horseradish Peroxidase; Hydrogen Peroxide; Indicators and Reagents; Methanol; Oxidation-Reduction | 2000 |
Regulation and evaluation of five methanol-inducible promoters in the methylotrophic yeast Candida boidinii.
Topics: Acid Phosphatase; Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Candida; Consensus Sequence; Enzyme Induction; Formate Dehydrogenases; Fungal Proteins; Gene Expression Regulation, Enzymologic; Genes, Reporter; Glucose; Membrane Proteins; Methanol; Peroxidases; Peroxiredoxins; Polymerase Chain Reaction; Promoter Regions, Genetic; Saccharomyces cerevisiae; Sequence Alignment | 2000 |
A methylotrophic pathway participates in pectin utilization by Candida boidinii.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Candida; Carboxylic Ester Hydrolases; Genes, Fungal; Kinetics; Methanol; Mutagenesis; Pectins; Peroxisomes; Polygalacturonase; Polysaccharide-Lyases; Substrate Specificity | 2000 |
[The measurement of intra-cellular AOX in recombinant Pichia pastoris].
Topics: Alcohol Oxidoreductases; Hydrogen-Ion Concentration; Methanol; Oxygen Consumption; Pichia; Recombination, Genetic | 2001 |
Isolation and properties of genetically defined strains of the methylotrophic yeast Hansenula polymorpha CBS4732.
Topics: Acebutolol; Alcohol Oxidoreductases; Catalase; Chromosome Segregation; Clone Cells; Meiosis; Methanol; Pichia | 2002 |
A new amperometric enzyme electrode for alcohol determination.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Electrochemistry; Electromagnetic Fields; Enzyme Stability; Enzymes, Immobilized; Equipment Design; Ferrous Compounds; Hydrogen-Ion Concentration; Methanol; Models, Chemical; Platinum; Polyvinyls; Sensitivity and Specificity; Temperature | 2002 |
Physiological role of the second alcohol oxidase gene MOD2 in the methylotrophic growth of Pichia methanolica.
Topics: Alcohol Oxidoreductases; Culture Media; Fungal Proteins; Gene Expression Regulation, Fungal; Isoenzymes; Methanol; Pichia | 2002 |
Old yellow enzyme confers resistance of Hansenula polymorpha towards allyl alcohol.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Drug Resistance, Microbial; Methanol; Molecular Sequence Data; Peroxisomes; Pichia; Propanols; Selection, Genetic; Sequence Homology, Amino Acid | 2002 |
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Topics: Alcohol Oxidoreductases; Apyrase; Base Sequence; Blotting, Western; Cations; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Genetic Vectors; Glycosylation; Methanol; Models, Chemical; Models, Genetic; Molecular Sequence Data; Mutagenesis; Oligonucleotides; Phosphates; Pichia; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Analysis, DNA; Solanum | 2003 |
Isolation of mutants of Hansenula polymorpha defective in the assembly of octameric alcohol oxidase.
Topics: Alcohol Oxidoreductases; Genetic Complementation Test; Methanol; Microscopy, Electron; Mutation; Peroxisomes; Pichia | 2002 |
Development of a bisphenol A-adsorbing yeast by surface display of the Kluyveromyces yellow enzyme on Pichia pastoris.
Topics: Adsorption; Alcohol Oxidoreductases; Artificial Gene Fusion; Benzhydryl Compounds; Biodegradation, Environmental; Culture Media; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Fungal; Hydrogen-Ion Concentration; Kluyveromyces; Mating Factor; Membrane Proteins; Methanol; NADPH Dehydrogenase; Peptides; Phenols; Pichia; Promoter Regions, Genetic; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins | 2004 |
[Expression of the human extracellular domain of high density lipoprotein receptor in methylotropic yeast].
Topics: Alcohol Oxidoreductases; Binding Sites; Binding, Competitive; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation; Humans; Lipoproteins, LDL; Methanol; Pichia; Plasmids; Promoter Regions, Genetic; Receptors, LDL; Reverse Transcriptase Polymerase Chain Reaction | 2003 |
A hexose transporter homologue controls glucose repression in the methylotrophic yeast Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Base Sequence; Biological Transport; Cloning, Molecular; Ethanol; Glucose; Maltose; Mannose; Methanol; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis; Mutation, Missense; Peroxisomes; Pichia; Point Mutation; Saccharomyces cerevisiae; Sequence Alignment; Sucrose; Trehalose | 2004 |
Real-time quantification of methanol in plants using a hybrid alcohol oxidase-peroxidase biosensor.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Calibration; Carboxylic Ester Hydrolases; Electrodes; Enzymes, Immobilized; Gas Chromatography-Mass Spectrometry; Horseradish Peroxidase; Hydrogen-Ion Concentration; Methanol; Molecular Structure; Nicotiana; Plant Extracts; Substrate Specificity; Temperature; Time Factors | 2004 |
Hansenula polymorpha Swi1p and Snf2p are essential for methanol utilisation.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Cloning, Molecular; DNA-Binding Proteins; DNA, Fungal; Fungal Proteins; Genes, Fungal; Genetic Complementation Test; Methanol; Microscopy, Electron; Molecular Sequence Data; Nuclear Proteins; Peroxisomes; Pichia; Promoter Regions, Genetic; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Transcription Factors | 2004 |
Comparison of three colorimetric reagents in the determination of methanol with alcohol oxidase. Application to the assay of pectin methylesterase.
Topics: Alcohol Oxidoreductases; Ammonia; Benzothiazoles; Carboxylic Ester Hydrolases; Colorimetry; Formaldehyde; Hydrazones; Indicators and Reagents; Methanol; Pentanones; Sensitivity and Specificity; Sulfhydryl Compounds; Thiazoles | 2004 |
A coupled spectrophotometric enzyme assay for the determination of pectin methylesterase activity and its inhibition by proteinaceous inhibitors.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Bacteria; Carboxylic Ester Hydrolases; Cell Wall; Citrus sinensis; Enzyme Inhibitors; Formaldehyde; Fruit; Fungi; Hydrogen-Ion Concentration; Methanol; Pectins; Spectrophotometry | 2004 |
Pectin utilization by the methylotrophic yeast Pichia methanolica.
Topics: Alcohol Oxidoreductases; Carboxylic Ester Hydrolases; Electrophoresis, Polyacrylamide Gel; Gene Deletion; Gene Expression; Methanol; Pectins; Peroxisomes; Pichia; RNA, Fungal; RNA, Messenger; Transcription, Genetic | 2005 |
Identification of four alcohol oxidases from methylotrophic yeasts.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Candida; Fungal Proteins; Methanol; Molecular Sequence Data; Phylogeny; Pichia; Sequence Analysis, DNA | 2005 |
Regulation of two distinct alcohol oxidase promoters in the methylotrophic yeast Pichia methanolica.
Topics: Acid Phosphatase; Alcohol Oxidoreductases; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Histocytochemistry; Methanol; Mutagenesis, Insertional; Oxygen; Pichia; Promoter Regions, Genetic | 2006 |
Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Cell Nucleus; Cloning, Molecular; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Fungal; Methanol; Molecular Sequence Data; Oleic Acid; Peroxisomes; Pichia; Promoter Regions, Genetic; Trans-Activators | 2006 |
Purification and characterization of alcohol oxidase from a genetically constructed over-producing strain of the methylotrophic yeast Hansenula polymorpha.
Topics: Alcohol Oxidoreductases; Ethanol; Formaldehyde; Fungal Proteins; Methanol; Molecular Weight; Pichia; Protein Structure, Secondary; Substrate Specificity | 2006 |
On-line monitoring of methanol in n-hexane by an organic-phase alcohol biosensor.
Topics: Alcohol Oxidoreductases; Biosensing Techniques; Gasoline; Hexanes; Methanol; Online Systems | 2007 |
Expression in and purification of Hepatitis B surface antigen (S-protein) from methylotrophic yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Animals; Biotechnology; Erythrocytes; Hemagglutination; Hepatitis B Surface Antigens; Humans; Methanol; Pichia; Promoter Regions, Genetic; Recombinant Proteins | 2006 |
Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures.
Topics: Alcohol Oxidoreductases; Avidin; Bioreactors; Glycerol; Industrial Microbiology; Methanol; Mycology; Pichia; Recombinant Proteins | 2007 |
The transcarboxylase domain of pyruvate carboxylase is essential for assembly of the peroxisomal flavoenzyme alcohol oxidase.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Aspartic Acid; DNA Transposable Elements; Fungal Proteins; Gene Deletion; Genetic Complementation Test; Methanol; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Pichia; Protein Binding; Protein Structure, Tertiary; Pyruvate Carboxylase | 2007 |
Peroxisomal metabolism is regulated by an oxygen-recognition system through organelle crosstalk between the mitochondria and peroxisomes.
Topics: Alcohol Oxidoreductases; Culture Media; Fungal Proteins; Gene Expression Regulation, Fungal; Isoenzymes; Methanol; Mitochondria; Oxygen; Peroxisomes; Pichia | 2007 |
Distribution, diversity and regulation of alcohol oxidase isozymes, and phylogenetic relationships of methylotrophic yeasts.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Candida; Fungal Proteins; Gene Expression Regulation, Fungal; Genetic Variation; Isoenzymes; Methanol; Molecular Sequence Data; Phylogeny; Pichia | 2007 |
Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies.
Topics: Alcohol Oxidoreductases; Biomass; Bioreactors; Cell Culture Techniques; Fermentation; Lipase; Lipolysis; Methanol; Microbiological Techniques; Pichia; Rhizopus; Salts; Temperature; Time Factors | 2007 |
Regulation of alcohol oxidase of a recombinant Pichia pastoris Mut+ strain in transient continuous cultures.
Topics: Alcohol Oxidoreductases; Food; Glycerol; Methanol; Organisms, Genetically Modified; Pichia; Time Factors | 2007 |
The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Carbohydrate Metabolism; DNA-Binding Proteins; DNA, Fungal; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Methanol; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Models, Biological; Molecular Sequence Data; Nuclear Proteins; Peroxisomes; Pichia; Repressor Proteins | 2007 |
An alcohol oxidase dipstick rapidly detects methanol in the serum of mice.
Topics: Alcohol Oxidoreductases; Animals; Color; Colorimetry; Cross Reactions; Disease Models, Animal; Ethanol; Ethylene Glycol; Methanol; Mice; Mice, Inbred C57BL; Point-of-Care Systems; Saliva; Time Factors | 2007 |
[Enzyme activity during reteplase production phase by recombinant Pichia pastoris].
Topics: Alcohol Oxidoreductases; Fermentation; Genetic Vectors; Glucosephosphate Dehydrogenase; Methanol; Pichia; Recombinant Proteins; Tissue Plasminogen Activator | 2008 |
Simple diagnostic tests to detect toxic alcohol intoxications.
Topics: 2-Propanol; Alcohol Dehydrogenase; Alcohol Oxidoreductases; Alcoholic Intoxication; Diagnosis, Differential; Ethanol; Ethylene Glycol; Ethylene Glycols; Humans; Methanol; Periodic Acid; Phosphoric Acids; Potassium Permanganate; Saliva | 2008 |
Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation.
Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Formate Dehydrogenases; Fungal Proteins; Genetic Engineering; Kinetics; Methanol; NAD; Oxidation-Reduction; Pichia | 2010 |
Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation.
Topics: Alcohol Oxidoreductases; Alkalies; Biomass; Fermentation; Intracellular Space; Methanol; Microbial Viability; Peptide Hydrolases; Pichia; Polysaccharide-Lyases; Protein Processing, Post-Translational; Sorbitol; Temperature; Time Factors | 2010 |
Hydrogen peroxide mediates the expression of ascorbate-related genes in response to methanol stimulation in Oncidium.
Topics: Alcohol Oxidoreductases; Ascorbic Acid; Gene Expression Regulation, Plant; Genes, Plant; Hexuronic Acids; Hydrogen Peroxide; Hydroxylamine; Methanol; NADPH Oxidases; Onium Compounds; Orchidaceae; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction | 2010 |
Dendritic chain reaction: responsive release of hydrogen peroxide upon generation and enzymatic oxidation of methanol.
Topics: Alcohol Oxidoreductases; Diagnostic Techniques and Procedures; Hydrogen Peroxide; Methanol; Molecular Probes; Oxidation-Reduction | 2010 |
Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii.
Topics: Alcohol Oxidoreductases; Aldehyde-Ketone Transferases; Candida; Chromatin Immunoprecipitation; DNA, Fungal; Ethanol; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Glucose; Methanol; Oleic Acid; Pichia; Promoter Regions, Genetic; Protein Binding; Saccharomyces cerevisiae; Sequence Homology, Amino Acid; Transcription Factors; Zinc Fingers | 2010 |
Improvement of porcine interferon-α production by recombinant Pichia pastoris via induction at low methanol concentration and low temperature.
Topics: Alcohol Oxidoreductases; Aldehyde Oxidoreductases; Animals; Bioreactors; Culture Media; Fermentation; Formate Dehydrogenases; Industrial Microbiology; Interferon-alpha; Methanol; Pichia; Recombinant Proteins; Swine; Temperature; Transcriptional Activation | 2011 |
Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol-inducible gene expression.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Candida; Cell Cycle; Cloning, Molecular; Fungal Proteins; Gene Expression Regulation, Fungal; Glucose; Methanol; Molecular Sequence Data; Repressor Proteins; Saccharomyces cerevisiae Proteins; Sequence Alignment | 2012 |
Heterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein.
Topics: 6-Phytase; Alcohol Oxidoreductases; Gene Expression; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycosylation; Hot Temperature; Methanol; Pichia; Polysaccharides; Promoter Regions, Genetic; Recombinant Proteins; Xylosidases | 2012 |
The zinc finger proteins Mxr1p and repressor of phosphoenolpyruvate carboxykinase (ROP) have the same DNA binding specificity but regulate methanol metabolism antagonistically in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Fungal Proteins; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Methanol; Pichia; Repressor Proteins | 2012 |
[Effect of nitrogen source on gene expression of first steps of methanol utilization pathway in Pichia pastoris].
Topics: Acid Phosphatase; Alcohol Oxidoreductases; Ammonium Sulfate; Catalase; Fungal Proteins; Gene Expression Regulation, Fungal; Glutamine; Methanol; Nitrogen; Pichia; Promoter Regions, Genetic; Saccharomyces cerevisiae Proteins; Urea | 2013 |
Comparative performance of S-adenosyl-L-methionine biosynthesis and degradation in Pichia pastoris using different promoters and novel consumption inhibitors.
Topics: Adenosine; Adenosine Triphosphate; Alcohol Oxidoreductases; Ethanol; Fermentation; Fungal Proteins; Genes, Synthetic; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenases; Hydrogen Peroxide; Methanol; Methionine Adenosyltransferase; Pichia; Promoter Regions, Genetic; Recombinant Fusion Proteins; S-Adenosylmethionine; Spermidine; Transcription, Genetic | 2014 |
Methanol-inducible promoter of thermotolerant methylotrophic yeast Ogataea thermomethanolica BCC16875 potential for production of heterologous protein at high temperatures.
Topics: Alcohol Oxidoreductases; DNA, Fungal; Gene Expression Regulation, Fungal; Hot Temperature; Methanol; Molecular Sequence Data; Promoter Regions, Genetic; Recombinant Proteins; Saccharomycetales; Sequence Analysis, DNA | 2014 |
Biofuel cell for generating power from methanol substrate using alcohol oxidase bioanode and air-breathed laccase biocathode.
Topics: Alcohol Oxidoreductases; Bioelectric Energy Sources; Electrodes; Electron Transport; Enzymes, Immobilized; Equipment Design; Laccase; Methanol; Models, Molecular; Nanotubes, Carbon; Osmium Tetroxide; Pichia; Polyvinyls; Trametes | 2014 |
Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance.
Topics: Alcohol Oxidoreductases; Aldose-Ketose Isomerases; Base Sequence; Bioreactors; Carbon; Codon; Fermentation; Hydrogen-Ion Concentration; Industrial Microbiology; Mannitol; Methanol; Molecular Sequence Data; Pichia; Recombinant Proteins; Sorbitol; Spectrophotometry, Ultraviolet; Temperature; Thermus thermophilus | 2015 |
Phase analysis in single-chain variable fragment production by recombinant Pichia pastoris based on proteomics combined with multivariate statistics.
Topics: Alcohol Oxidoreductases; Aspartic Acid Endopeptidases; Batch Cell Culture Techniques; C-Reactive Protein; Electrophoresis, Gel, Two-Dimensional; Methanol; Multivariate Analysis; Peptide Mapping; Pichia; Proteomics; Recombinant Proteins; Single-Chain Antibodies | 2015 |
Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter.
Topics: Alcohol Oxidoreductases; Culture Media; Fungal Proteins; Gene Expression Regulation, Fungal; Glucose; Glycerol; Industrial Microbiology; Methanol; Pichia; Promoter Regions, Genetic; Sorbitol | 2015 |
Simplified high-throughput screening of AOX1-expressed laccase enzyme in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Benzothiazoles; Bleomycin; Clone Cells; Colorimetry; Coloring Agents; Enzyme Induction; Fungal Proteins; Gene Library; Kinetics; Laccase; Methanol; Mitochondrial Proteins; Mutagenesis; Mutant Proteins; Oxidoreductases; Pichia; Plant Proteins; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Engineering; Recombinant Proteins; Sulfonic Acids | 2015 |
Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Amino Acid Sequence; Binding Sites; Enzyme Repression; Fungal Proteins; Gene Expression Regulation, Fungal; Methanol; Molecular Sequence Data; Peroxisomes; Pichia; Promoter Regions, Genetic; Protein Transport; Sequence Homology, Amino Acid; Signal Transduction; Transcription Factors | 2016 |
GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris.
Topics: Alcohol Oxidoreductases; Bioreactors; Fermentation; Glyceraldehyde 3-Phosphate; Methanol; Pichia; Promoter Regions, Genetic; Streptavidin | 2016 |
Regulation of intracellular formaldehyde toxicity during methanol metabolism of the methylotrophic yeast Pichia methanolica.
Topics: Alcohol Oxidoreductases; Cytoplasm; Cytoprotection; Formaldehyde; Gene Expression Regulation, Fungal; Metabolic Networks and Pathways; Methanol; Pichia; Saccharomyces cerevisiae | 2016 |
A novel methanol-free Pichia pastoris system for recombinant protein expression.
Topics: Alcohol Oxidoreductases; Dihydroxyacetone; Gene Knockout Techniques; Glycerol; Glycerol Kinase; Methanol; Pichia; Recombinant Proteins | 2016 |
Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris.
Topics: Alcohol Oxidoreductases; Fungal Proteins; Gene Dosage; Gene Expression Regulation, Fungal; Lipase; Methanol; Peroxisomes; Pichia; Principal Component Analysis; Promoter Regions, Genetic; Rhizopus; Transcription, Genetic; Transcriptome | 2017 |
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Batch Cell Culture Techniques; Biotechnology; Cloning, Molecular; Culture Media; Directed Molecular Evolution; Methanol; Mutation; Pichia; Pilot Projects; Promoter Regions, Genetic; Recombinant Proteins | 2017 |
Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages.
Topics: Alcohol Oxidoreductases; Alcoholic Beverages; Biosensing Techniques; Cadmium Compounds; Cysteine; Electrochemical Techniques; Enzymes, Immobilized; Hydrogen Peroxide; Limit of Detection; Methanol; Oxidation-Reduction; Pichia; Quantum Dots; Sulfides | 2018 |
Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris.
Topics: Alcohol Oxidoreductases; Cell Engineering; Fermentation; Insulin; Methanol; Oxygen; Pichia; Promoter Regions, Genetic; Protein Precursors; Recombinant Proteins | 2018 |
Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris.
Topics: Alcohol Oxidoreductases; Autophagy; Catabolite Repression; Gene Expression Profiling; Gene Expression Regulation, Fungal; Metabolic Networks and Pathways; Methanol; Peroxisomes; Pichia; Promoter Regions, Genetic; Repressor Proteins; Saccharomyces cerevisiae Proteins; Transcription Factors | 2018 |
Synthesis and characterization of Ogataea thermomethanolica alcohol oxidase immobilized on barium ferrite magnetic microparticles.
Topics: Alcohol Oxidoreductases; Barium Compounds; Biocatalysis; Chemistry Techniques, Synthetic; Enzyme Stability; Enzymes, Immobilized; Ferric Compounds; Hydrogen-Ion Concentration; Magnets; Methanol; Microspheres; Saccharomycetales; Temperature | 2019 |
Efficient Development of a Mixed Feed Process for Pichia pastoris.
Topics: Alcohol Oxidoreductases; Biomass; Methanol; Pichia; Recombinant Proteins | 2019 |
Identification of major ADH genes in ethanol metabolism of Pichia pastoris.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Culture Media; Escherichia coli; Ethanol; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Fungal; Glucose; Glycerol; Methanol; Pichia; Promoter Regions, Genetic; Recombinant Proteins; Sequence Analysis, Protein; Sequence Homology, Amino Acid | 2020 |
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P
Topics: Alcohol Oxidoreductases; Fungal Proteins; Gene Dosage; Gene Expression; Gene Expression Regulation, Fungal; Methanol; Pichia; Promoter Regions, Genetic; Recombinant Proteins | 2019 |
Fructose-1,6-bisphosphatase degradation in the methylotrophic yeast Komagataella phaffii occurs in autophagy pathway.
Topics: Alcohol Oxidoreductases; Autophagy; beta-Galactosidase; Cytosol; Fructose-Bisphosphatase; Methanol; Plasmids; Proteolysis; Saccharomycetales | 2021 |
Biomass soft sensor for a Pichia pastoris fed-batch process based on phase detection and hybrid modeling.
Topics: Alcohol Oxidoreductases; Algorithms; Batch Cell Culture Techniques; Biomass; Bioreactors; Carbon Dioxide; Glycerol; Methanol; Models, Biological; Recombinant Proteins; Saccharomycetales | 2020 |
Beyond alcohol oxidase: the methylotrophic yeast Komagataella phaffii utilizes methanol also with its native alcohol dehydrogenase Adh2.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Fungal Proteins; Gene Expression Regulation, Fungal; Methanol; Promoter Regions, Genetic; Recombinant Proteins; Saccharomycetales | 2021 |
Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataea minuta.
Topics: Acid Phosphatase; Alcohol Oxidoreductases; Deoxyribonucleases; Gene Expression; Gene Expression Regulation, Fungal; Methanol; Pichia; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Saccharomycetales; tRNA Methyltransferases | 2021 |