metaphosphoric-acid and 1-10-phenanthroline

metaphosphoric-acid has been researched along with 1-10-phenanthroline* in 1 studies

Other Studies

1 other study(ies) available for metaphosphoric-acid and 1-10-phenanthroline

Article	Year
Determination of reduced and oxidized glutathione in erythrocytes by high-performance liquid chromatography with ultraviolet absorbance detection. Journal of chromatography. B, Biomedical applications, 1996, Apr-12, Volume: 678, Issue:2 A method is described for simultaneous quantitation of reduced (GSH) and oxidized (GSSG) glutathione in erythrocytes by HPLC. They were determined by standard addition method. Blood samples were collected in tubes containing 1,10-phenanthroline. The separated erythrocytes were hemolyzed with water containing standard. After deproteinization, GSH and GSSG were converted to N-(2,4-dinitrophenyl) derivatives and analyzed by HPLC with UV detection. The coefficients of variation of GSH and GSSG on replicate assays were 6% and 8%, respectively. The stabilities of GSH and GSSG and of the derivatives were also examined. The present method appears to be satisfactory for determination of these physiological concentrations in erythrocytes. Topics: Blood Proteins; Chromatography, High Pressure Liquid; Drug Stability; Erythrocytes; Ferric Compounds; Glutathione; Humans; Oxidation-Reduction; Phenanthrolines; Phosphorous Acids; Sensitivity and Specificity	1996

Article

Year

Determination of reduced and oxidized glutathione in erythrocytes by high-performance liquid chromatography with ultraviolet absorbance detection.

Journal of chromatography. B, Biomedical applications, 1996, Apr-12, Volume: 678, Issue:2

A method is described for simultaneous quantitation of reduced (GSH) and oxidized (GSSG) glutathione in erythrocytes by HPLC. They were determined by standard addition method. Blood samples were collected in tubes containing 1,10-phenanthroline. The separated erythrocytes were hemolyzed with water containing standard. After deproteinization, GSH and GSSG were converted to N-(2,4-dinitrophenyl) derivatives and analyzed by HPLC with UV detection. The coefficients of variation of GSH and GSSG on replicate assays were 6% and 8%, respectively. The stabilities of GSH and GSSG and of the derivatives were also examined. The present method appears to be satisfactory for determination of these physiological concentrations in erythrocytes.

Topics: Blood Proteins; Chromatography, High Pressure Liquid; Drug Stability; Erythrocytes; Ferric Compounds; Glutathione; Humans; Oxidation-Reduction; Phenanthrolines; Phosphorous Acids; Sensitivity and Specificity

1996