metallothionein and methyl-jasmonate

This study investigated the effects of methyl jasmonate (MeJA) on chlorophyll concentration, lipid peroxidation, Cd uptake, antioxidative capacity, and type-2 metallothionein gene (KoMT2) expression in the leaves of Kandelia obovata seedlings exposed to Cd stress. Deleterious effects, including decreased chlorophyll content and increased malondialdehyde concentration, were observed in leaves of K. obovata after 9d of 200μmol L(-1) Cd treatment. Application of MeJA (0.1 to 1μmol L(-1)) increased the concentration of ascorbic acid and the activities of catalase and ascorbate peroxidase in the leaves of K. obovata, which helped alleviate the oxidative damage induced by Cd stress. The concentration of endogenous jasmonic acid in the leaves of K. obovata was decreased by Cd but was positively stimulated by exogenous MeJA. The expression of KoMT2 in the leaves was enhanced after 9d of 200μmol L(-1) Cd treatment, while the exogenous application of MeJA significantly restored the expression of KoMT2. Exogenous MeJA also inhibited the uptake of Cd to the aboveground part (leaves) of the seedlings, which helped reduce direct damages of Cd to the photosynthetic organ of the plant. The reduced uptake of Cd might be a result of stomatal closure and decreased transpiration by exogenous MeJA.

Topics: Acetates; Cadmium; Cyclopentanes; Enzyme Activation; Enzymes; Gene Expression Regulation, Plant; Lipid Peroxidation; Malondialdehyde; Metallothionein; Oxylipins; Plant Growth Regulators; Plant Leaves; Plant Roots; Rhizophoraceae; Seedlings; Soil Pollutants; Stress, Physiological

The expression profiles of three Brassica rapa metallothionein genes (BrMT 1-3) were determined in 7-day-old seedlings exposed to various exogenous factors including plant hormones, heavy metals and abiotic stresses. BrMT1, BrMT2, and BrMT3 were representatives of MT gene type 1, type 2, and type 3, respectively, according to their cysteine alignment. BrMT2 showed a relatively higher basal expression level compared to BrMT1 and BrMT3 under normal conditions. The BrMT1 transcript was markedly increased by various factors including ethephon, polyethylene glycol and hydrogen peroxide, with no down-regulation evident. On the contrary, BrMT2 expression was down-regulated by abscisic acid, salicylic acid, and methyl jasmonate. Heavy metals did not increase BrMT2 expression. BrMT3 expression was only marginally and non-significantly up- and down-regulated by the stress conditions tested. Promoter regions of BrMT1 and BrMT2 display different cis-acting elements supporting the different responses of both genes against various stresses. The results demonstrate the differential regulation of BrMT1-3 by various plant exogenous factors, and indicate the utility of the BrMT1 promoter as a multiple stress inducible promoter.

Topics: Abscisic Acid; Acetates; Brassica rapa; Cyclopentanes; DNA Primers; Gene Expression Profiling; Gene Expression Regulation, Plant; Hydrogen Peroxide; Metallothionein; Metals, Heavy; Organophosphorus Compounds; Oxylipins; Plant Growth Regulators; Polyethylene Glycols; Reverse Transcriptase Polymerase Chain Reaction; Salicylic Acid; Seedlings