metallothionein and indoleacetic-acid

Although many bacteria are tolerant to heavy metals and play important roles in the immobilization of heavy metals, they cannot always be dependably reproduced under field conditions. In this work, a cadmium (Cd)-resistant bacterium was isolated from a Cd-contaminated oil field and identified as Pseudomonas aeruginosa (Pse-w). We then determined various plant growth promoting features such as the solubilization of phosphate, and the production of indole-3-acetic acid and siderophores. Lastly, we engineered the strain Pse-w-MT by targeting metallothioneins to the cell surface of Pse-w to immobilize Cd

Topics: Agricultural Inoculants; Biodegradation, Environmental; Biomass; Cadmium; Genetic Engineering; Indoleacetic Acids; Metallothionein; Plant Development; Plant Leaves; Plant Roots; Plants; Pseudomonas aeruginosa; Rhizobium; Siderophores; Soil Microbiology; Soil Pollutants

Physcomitrella patens is a model plant for studying gene function using a knockout strategy. To establish a proteome database for P. patens, we resolved over 1,500 soluble proteins from gametophore and protonema tissues by two-dimensional electrophoresis (2-DE) and obtained peptide mass fingerprints (PMFs) by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Using expressed sequence tags (ESTs), we were able to predict the identities of 90 protein spots. Most of these were related to energy or primary metabolism. Comparative proteome analysis was used to identify proteins specific for each of the tissue types. One of these was a metallothionein type-2 (PpMT2) protein that was highly upregulated in gametophore tissue. PpMT2 was induced in both the gametophore and protonema following culture on solid media and in response to various abiotic stresses such as copper, cadmium, cold, indole-3-acetic acid, and ethylene. We suggest that PpMT2 is not only involved in metal binding and detoxification, but also in many biological aspects as a metal messenger or a protein with additional functions.

Topics: Amino Acid Sequence; Bryopsida; Cadmium; Cold Temperature; Copper; Electrophoresis, Gel, Two-Dimensional; Ethylenes; Expressed Sequence Tags; Gene Expression Regulation, Plant; Indoleacetic Acids; Metallothionein; Molecular Sequence Data; Plant Proteins; Proteome; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization