metallothionein and epostane

An increase in metallothionein 1 (MT-1) mRNA was detected in the ovaries of immature Wistar rats that were primed with s.c. injection of 10 IU eCG followed 48 h later by 10 IU hCG s.c. to initiate the ovulatory process. Ovarian RNA was extracted at 0, 2, 4, 8, 12, 24, 72, 144, and 288 h after the primed animals were injected with hCG. These extracts were used for reverse transcription polymerase chain reaction (RT-PCR) differential display and Northern analyses that yielded complementary gene fragments for MT-1. Expression of MT-1 mRNA increased significantly by 24 h after hCG treatment and reached a peak at 144 h after hCG. In contrast, a disintegrin and metalloproteinase with thrombospondin motifs and a tissue inhibitor of metalloproteinase 1, which were also detected by the RT-PCR differential display procedure, reached a peak at 12 h after hCG and returned to control levels in the ovaries by 72 h after hCG. In situ hybridization indicated that most of the MT-1 mRNA was expressed in the vicinity of the theca interna of preovulatory follicles and in the lutein granulosa of postovulatory follicles. Thus, MT-1 mRNA expression is primarily in the vicinity of steroid-secreting areas of the ovary. The substantial increase in MT-1 mRNA expression might be important in protecting the ovarian tissues from oxidative stress generated by ovarian inflammatory events during the ovulatory process and luteinization.

Topics: Abortifacient Agents; ADAM Proteins; ADAMTS1 Protein; Androstenols; Animals; Blotting, Northern; Corpus Luteum; Cyclooxygenase Inhibitors; Dinoprostone; Disintegrins; DNA, Complementary; Female; In Situ Hybridization; Indomethacin; Metalloendopeptidases; Metallothionein; Ovarian Follicle; Ovary; Ovulation; Progesterone; Rats; Rats, Wistar; RNA, Messenger; Steroids; Tissue Inhibitor of Metalloproteinase-1; Transcription, Genetic