metallothionein has been researched along with 2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid* in 2 studies
2 other study(ies) available for metallothionein and 2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid
| Article | Year |
|---|---|
Vanillin mitigates potassium bromate-induced molecular, biochemical and histopathological changes in the kidney of adult mice.
The present study aimed to explore the ability of vanillin to ameliorate the adverse effects induced by potassium bromate (KBrO3) in the renal tissue. Our results showed a significant increase in hydrogen peroxide, superoxide anion, malondialdehyde, advanced oxidation protein product and protein carbonyl levels in the kidney of KBrO3 treated mice, compared with the control group. Nephrotoxicity was evidenced by a decrease in plasma uric acid and kidney glutathione levels, Na(+)-K(+)-ATPase, lactate dehydrogenase and catalase activities. Additionally, creatinine and urea levels significantly increased in the plasma and declined in the urine. Also, Kidney glutathione peroxidase, superoxide dismutase, metallothionein (MT1 and MT2) mRNA expression remarkably increased. These modifications in biochemical and molecular values were substantiated by histopathological data. Co-treatment with vanillin restored these parameters to near control values. Interestingly, vanillin proved to possess, in vitro, a stronger scavenging radical activity than vitamin C and Trolox. Thus, vanillin inhibited KBrO3-induced damage via its antioxidant and antiradical activities as well as its capacity to protect genes expression and histopathological changes. Topics: Adenosine Triphosphatases; Animals; Antioxidants; Benzaldehydes; Benzothiazoles; Biphenyl Compounds; Bromates; Environmental Pollutants; Hair Preparations; Kidney; Lipid Peroxidation; Metallothionein; Mice; Oxidative Stress; Picrates; Sulfonic Acids; Up-Regulation | 2016 |
In vitro free radical scavenging activity of hepatic metallothionein induced in an Indian freshwater fish, Channa punctata Bloch.
Mammalian metallothioneins (MT) have been reported to scavenge free radicals. There is no experimental evidence to show that fish MT has a similar property. In the present study cadmium-induced MT (Cd-MT) from the liver of an Indian freshwater fish Channa punctata Bloch was investigated for its free radical scavenging activity using three different in vitro assays. Exposure to cadmium chloride (0.2 mg/kg body weight; three doses on alternate days) resulted in a marked induction of Cd-MT in liver. Only a single isoform of Cd-MT was found to be induced. Molecular weight of Cd-MT was found to be 14 kDa as deduced by SDS-PAGE analysis. The purified Cd-MT effectively scavenged the following free radicals: superoxide radical (O2*-), 2,2'-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS*+) and 1,1-diphenyl-picrylhydrazyl radical (DPPH*). The radical scavenging effect was found to be concentration-dependent. Also, the purified MT exhibited an inhibitory effect on ferric nitrilotriacetate (Fe-NTA) induced oxidative DNA damage in vitro. The cysteine residues of MT are proposed to be the main candidate for its radical scavenging activity. Findings of the present study strongly suggest a free radical scavenging role for fish MT. Present study adds to the little existing knowledge about fish MT and its possible biological functions. Topics: Animals; Benzothiazoles; Biphenyl Compounds; Cadmium; DNA; DNA Damage; Ferric Compounds; Free Radical Scavengers; Free Radicals; Hydrazines; Kidney; Liver; Metallothionein; Molecular Weight; Muscle, Skeletal; Nitrilotriacetic Acid; Nitrites; Perciformes; Picrates; Sulfonic Acids; Superoxides | 2006 |