meropenem and ammonium-bicarbonate

meropenem has been researched along with ammonium-bicarbonate* in 1 studies

Other Studies

1 other study(ies) available for meropenem and ammonium-bicarbonate

Article	Year
Matrix-assisted laser desorption ionization-time of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity. Journal of clinical microbiology, 2015, Volume: 53, Issue:5 A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers. Topics: Automation, Laboratory; Bacterial Proteins; beta-Lactamases; Bicarbonates; Buffers; Electronic Data Processing; Humans; Hydrolysis; Meropenem; Sensitivity and Specificity; Software; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thienamycins	2015

Article

Year

Matrix-assisted laser desorption ionization-time of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity.

Journal of clinical microbiology, 2015, Volume: 53, Issue:5

A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.

Topics: Automation, Laboratory; Bacterial Proteins; beta-Lactamases; Bicarbonates; Buffers; Electronic Data Processing; Humans; Hydrolysis; Meropenem; Sensitivity and Specificity; Software; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thienamycins

2015