merocyanine-dye and lysophosphatidic-acid

merocyanine-dye has been researched along with lysophosphatidic-acid* in 1 studies

Other Studies

1 other study(ies) available for merocyanine-dye and lysophosphatidic-acid

ArticleYear
A biosensor of S100A4 metastasis factor activation: inhibitor screening and cellular activation dynamics.
    Biochemistry, 2008, Jan-22, Volume: 47, Issue:3

    S100A4, a member of the S100 family of Ca2+-binding proteins, displays elevated expression in malignant human tumors compared with benign tumors, and increased expression correlates strongly with poor patient survival. S100A4 has a direct role in metastatic progression, likely due to the modulation of actomyosin cytoskeletal dynamics, which results in increased cellular motility. We developed a fluorescent biosensor (Mero-S100A4) that reports on the Ca2+-bound, activated form of S100A4. Direct attachment of a novel solvatochromatic reporter dye to S100A4 results in a sensor that, upon activation, undergoes a 3-fold enhancement in fluorescence, thus providing a sensitive assay for use in vitro and in vivo. In cells, localized activation of S100A4 at the cell periphery is observed during random migration and following stimulation with lysophosphatidic acid, a known activator of cell motility and proliferation. Additionally, a screen against a library of FDA-approved drugs with the biosensor identified an array of phenothiazines as inhibitors of myosin-II associated S100A4 function. These data demonstrate the utility of the new biosensor both for drug discovery and for probing the cellular dynamics controlled by the S100A4 metastasis factor.

    Topics: Animals; Biosensing Techniques; Calcium; Cell Movement; Cell-Free System; Circular Dichroism; Cysteine; Egtazic Acid; Fibroblasts; Fluorescence Polarization; Humans; Iodoacetamide; Lysophospholipids; Mice; Models, Molecular; Molecular Structure; Nonmuscle Myosin Type IIA; Phenothiazines; Protein Binding; Protein Conformation; Pyrimidinones; Recombinant Proteins; S100 Calcium-Binding Protein A4; S100 Proteins; Spectrometry, Fluorescence; Trifluoperazine

2008