mercaptopurine and 4-aminothiophenol

In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.

Topics: Aniline Compounds; Antibodies, Immobilized; Biomarkers; ErbB Receptors; Humans; Immunoassay; Mercaptopurine; Microscopy; Nanostructures; Silver; Spectrum Analysis, Raman; Sulfhydryl Compounds; Surface Properties; Tumor Suppressor Protein p53